Editorial: About the Foodborne Pathogen Campylobacter

Tresse, Odile; Álvarez‐Ordóñez, Avelino; Connerton, Ian F.

doi:10.3389/fmicb.2017.01908

Cited by 38 publications

(33 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Campylobacter are widespread in the environment and they are a part of the natural microflora of birds and other animals. The main transmission route of these microorganisms to humans is by consumption and handling of contaminated food, especially of poultry origin [2][3][4]. Chicken carcasses are being frequently contaminated in the abattoirs, especially by direct contact with intestinal content of slaughtered chickens which may contain up to 10 8 of Campylobacter cells per gram [2,5].…”

Section: Introductionmentioning

confidence: 99%

MLST-based genetic relatedness of Campylobacter jejuni isolated from chickens and humans in Poland

2020

View full text Add to dashboard Cite

Campylobacter jejuni infection is one of the most frequently reported foodborne bacterial diseases worldwide. The main transmission route of these microorganisms to humans is consumption of contaminated food, especially of chicken origin. The aim of this study was to analyze the genetic relatedness of C. jejuni from chicken sources (feces, carcasses, and meat) and from humans with diarrhea as well as to subtype the isolates to gain better insight into their population structure present in Poland. C. jejuni were genotyped using multilocus sequence typing (MLST) and sequence types (STs) were assigned in the MLST database. Among 602 isolates tested, a total of 121 different STs, including 70 (57.9%) unique to the isolates' origin, and 32 STs that were not present in the MLST database were identified. The most prevalent STs were ST464 and ST257, with 58 (9.6%) and 52 (8.6%) C. jejuni isolates, respectively. Isolates with some STs (464, 6411, 257, 50) were shown to be common in chickens, whereas others (e.g. ST21 and ST572) were more often identified among human C. jejuni. It was shown that of 47 human sequence types, 26 STs (106 isolates), 23 STs (102 isolates), and 29 STs (100 isolates) were also identified in chicken feces, meat, and carcasses, respectively. These results, together with the high and similar proportional similarity indexes (PSI) calculated for C. jejuni isolated from patients and chickens, may suggest that human campylobacteriosis was associated with contaminated chicken meat or meat products or other kinds of food cross-contaminated with campylobacters of chicken origin. The frequency of various sequence types identified in the present study generally reflects of the prevalence of STs in other countries which may suggest that C. jejuni with some STs have a global distribution, while other genotypes may be more restricted to certain countries.

show abstract

Section: Introductionmentioning

confidence: 99%

MLST-based genetic relatedness of Campylobacter jejuni isolated from chickens and humans in Poland

2020

View full text Add to dashboard Cite

show abstract

“…Campylobacter , mainly Campylobacter jejuni , is one of the most common causes of foodborne bacterial infections worldwide (Allos, 2001 ; Bolton, 2015 ; Kaakoush et al, 2015 ; Tresse et al, 2017 ). Campylobacteriosis is also the most commonly reported zoonosis in the European Union (EU) with 246,307 confirmed cases and the notification rate 66.3 per 100,000 population in 2016 [EFSA (European Food Safety Authority) and ECDC (European Centre for Disease Prevention and Control), 2017 ].…”

Section: Introductionmentioning

confidence: 99%

“…The majority of the infections (83.6%) was caused by C. jejuni [EFSA (European Food Safety Authority) and ECDC (European Centre for Disease Prevention and Control), 2017 ]. The main transmission route of Campylobacter to humans is handling, preparation and consumption of contaminated food, especially of poultry origin (Allos, 2001 ; Park, 2002 ; Humphrey et al, 2007 ; Tresse et al, 2017 ). C. jejuni does not cause clinical diseases in poultry, but poultry carcasses have been frequently contaminated in the slaughterhouse due to a high prevalence of these bacteria in the intestinal tract of chickens; therefore, poultry carcasses can serve as the source of these microorganisms to humans (Humphrey et al, 2007 ; van Gerwe et al, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Resistance and Virulence-Associated Traits of Campylobacter jejuni Isolated From Poultry Food Chain and Humans With Diarrhea

Wieczorek¹,

Wołkowicz²,

Osek³

2018

Front. Microbiol.

View full text Add to dashboard Cite

The objective of this study was to test the prevalence of virulence-associated markers and antimicrobial resistance in 624 C. jejuni isolated from poultry food chain, i. e., chicken feces (n = 160), poultry carcasses (n = 157), poultry meat (n = 152) and from humans (n = 155). All human strains were positive for 9 out of 13 putative virulence genes responsible for expression of pathogenic factors involved in different stages of the infection. The presence of all markers was also high in strains from chicken feces, carcasses and meat although not all of them were identified in 100% of the isolates. On the other hand, the virB11, wlaN, and iam putative pathogenic genes were detected in only 1.9, 15.2, and 20.5% of strains, respectively. C. jejuni isolates, irrespective of the origin, were highly resistant to ciprofloxacin (92.5% isolates), followed by nalidixic acid (88.9%) and tetracycline (68.4%). In case of ciprofloxacin, significantly more isolates from poultry feces, carcasses and meat were resistant than those obtained from humans and the same relationship was observed for tetracycline where the isolates from chicken feces were more often resistant than C. jejuni of carcasses and meat origin. A low number of strains was resistant to streptomycin (18.4% isolates) and only 5 strains (0.8%) displayed resistance to erythromycin. A relationship between resistance to fluoroquinolones and presence of selected pathogenic markers was observed, e.g., from 83.3% strains with the virB11 to 93.4% with the docA genes were resistant to ciprofloxacin. The isolates that did not possess any of the pathogenic traits were also mainly resistant to this antimicrobial, although the number of such strains was usually low, except virB11 (612 isolates), wlaN (529 strains), and iam (496 isolates). Furthermore, resistance to tetracycline was somehow associated with the presence of the virulence associated genes wlaN and virB11 (56.8 and 75.0% isolates, respectively). The present study shows a high antimicrobial resistance to quinolones and tetracycline of C. jejuni isolated along poultry food chain and from patients with diarrhea, which was closely correlated with the presence of several virulence genes playing a role in the pathogenesis of Campylobacter infection.

show abstract

“…This infection has surpassed Salmonella several years ago and caused a significant economic burden ( EFSA and ECDC, 2016 ). Although new species of Campylobacter have recently been discovered, human campylobacteriosis are dominated by two main species, Campylobacter jejuni and Campylobacter coli ( Tresse et al, 2017 ). Campylobacter infection causes watery diarrhea, abortion, human acute enteritis, and several complications, such as Guillain-Barré syndrome and Reiter’s syndrome, in severe cases.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Characteristics of Campylobacter Throughout the Slaughter Process of Different Broiler Batches

et al. 2018

View full text Add to dashboard Cite

Handling and consumption of chicken meat are risk factors for human campylobacteriosis. This study was performed to describe the Campylobacter population in broiler carcasses and environmental samples throughout the slaughter process. Moreover, the genetic diversity and antimicrobial resistance of the Campylobacter strains were evaluated. Cloacal swabs, samples from carcasses at different stages, and environmental samples were collected thrice from the different flocks at the same abattoir located in Central Jiangsu, China. Campylobacter isolated from the three batches (n = 348) were identified as Campylobacter jejuni (n = 117) and Campylobacter coli (n = 151) by multiplex PCR. Characterization by multilocus sequence typing revealed a specific genotype of Campylobacter for each batch. Antimicrobial sensitivity to 18 antibiotics were analyzed for all selected strains according to the agar diffusion method recommended by the Clinical and Laboratory Standards Institute. Antibiotic susceptibility tests indicated that the majority of the tested isolates were resistant to quinolones (>89.7%). Less resistance to macrolide (59.8%), gentamicin (42.7%), amikacin (36.8%) was observed. Results showed that 94.0% of the tested strains demonstrated multidrug resistance.

show abstract

Editorial: About the Foodborne Pathogen Campylobacter

Cited by 38 publications

References 16 publications

MLST-based genetic relatedness of Campylobacter jejuni isolated from chickens and humans in Poland

MLST-based genetic relatedness of Campylobacter jejuni isolated from chickens and humans in Poland

Antimicrobial Resistance and Virulence-Associated Traits of Campylobacter jejuni Isolated From Poultry Food Chain and Humans With Diarrhea

Prevalence and Characteristics of Campylobacter Throughout the Slaughter Process of Different Broiler Batches

Contact Info

Product

Resources

About