Identification and utilization of a new Erysiphe necator isolate NAFU1 to quickly evaluate powdery mildew resistance in wild Chinese grapevine species using detached leaves

Gao, Yurong; Han, Yujun; Zhao, Fengli; Li, Yajuan; Cheng, Yuan; Ding, Qin; Wang, Yuejin; Wen, Ying-Qiang

doi:10.1016/j.plaphy.2015.11.003

Cited by 34 publications

(31 citation statements)

References 29 publications

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“…It is worth noticing that Erysisphe declined sharply in B, from 93% (Y11) in July to 1% (Y13) in October. This finding was consistent with an earlier report, which indicated that grapevine powdery mildew is one of the most damaging fungal diseases and it often occurs during July [ 42 ], suggesting that grapevine powdery mildew in B is quite serious and needs proper preventive measures [ 43 – 44 ]. Meanwhile, Erysisphe was found in Z. Interestingly, the Penicillium increased from 2% (Y11) to 44% (Y13), had become a dominant genus in B of Y samples in October.…”

Section: Resultssupporting

confidence: 93%

High-throughput sequencing of microbial community diversity in soil, grapes, leaves, grape juice and wine of grapevine from China

Yan

et al. 2018

PLoS ONE

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In this study Illumina MiSeq was performed to investigate microbial diversity in soil, leaves, grape, grape juice and wine. A total of 1,043,102 fungal Internal Transcribed Spacer (ITS) reads and 2,422,188 high quality bacterial 16S rDNA sequences were used for taxonomic classification, revealed five fungal and eight bacterial phyla. At the genus level, the dominant fungi were Ascomycota, Sordariales, Tetracladium and Geomyces in soil, Aureobasidium and Pleosporaceae in grapes leaves, Aureobasidium in grape and grape juice. The dominant bacteria were Kaistobacter, Arthrobacter, Skermanella and Sphingomonas in soil, Pseudomonas, Acinetobacter and Kaistobacter in grape and grapes leaves, and Oenococcus in grape juice and wine. Principal coordinate analysis showed structural separation between the composition of fungi and bacteria in all samples. This is the first study to understand microbiome population in soil, grape, grapes leaves, grape juice and wine in Xinjiang through High-throughput Sequencing and identify microorganisms like Saccharomyces cerevisiae and Oenococcus spp. that may contribute to the quality and flavor of wine.

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Section: Resultssupporting

confidence: 93%

High-throughput sequencing of microbial community diversity in soil, grapes, leaves, grape juice and wine of grapevine from China

Yan

et al. 2018

PLoS ONE

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“…5b), and detached leaves of these plants were inoculated with the powdery mildew isolate En. NAFU1 33 15 days after transplantation. Leaves inoculated with En.…”

Section: Powdery Mildew-triggered Mesophyll Cell Death Cell Wall Appmentioning

confidence: 99%

“…Four MLO-edited lines, CM3G1-51 and -91 and CM3G2-30 and -40, were assessed for resistance to powdery mildew. The transgenic mutant and nontransgenic control lines were inoculated with the powdery mildew isolate En NAFU1 as described previously 33 . For detached leaf inoculation, CM3G2-30, CM3G1-51, and wild-type (WT) plants were used at 15 days after transplantation from the subculture medium.…”

Section: Pathogen Inoculation and Evaluation Of Resistance To Powderymentioning

confidence: 99%

CRISPR/Cas9-mediated mutagenesis of VvMLO3 results in enhanced resistance to powdery mildew in grapevine (Vitis vinifera)

et al. 2020

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Grapevine (Vitis vinifera), one of the most economically important fruit crops in the world, suffers significant yield losses from powdery mildew, a major fungal disease caused by Erysiphe necator. In addition to suppressing host immunity, phytopathogens modulate host proteins termed susceptibility (S) factors to promote their proliferation in plants. In this study, CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated 9) technology was used to enable the targeted mutagenesis of MLO (mildew resistance Locus O) family genes that are thought to serve as S factors for powdery mildew fungi. Small deletions or insertions were induced in one or both alleles of two grapevine MLO genes, VvMLO3 and VvMLO4, in the transgenic plantlets of the powdery mildew-susceptible cultivar Thompson Seedless. The editing efficiency achieved with different CRISPR/Cas9 constructs varied from 0 to 38.5%. Among the 20 VvMLO3/4-edited lines obtained, one was homozygous for a single mutation, three harbored biallelic mutations, seven were heterozygous for the mutations, and nine were chimeric, as indicated by the presence of more than two mutated alleles in each line. Six of the 20 VvMLO3/4-edited grapevine lines showed normal growth, while the remaining lines exhibited senescence-like chlorosis and necrosis. Importantly, four VvMLO3-edited lines showed enhanced resistance to powdery mildew, which was associated with host cell death, cell wall apposition (CWA) and H 2 O 2 accumulation. Taken together, our results demonstrate that CRISPR/Cas9 genome-editing technology can be successfully used to induce targeted mutations in genes of interest to improve traits of economic importance, such as disease resistance in grapevines.

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“…Light microscopy can distinguish hyphae, appressoria, conidia and conidiophores, while haustoria cannot be monitored (Cadle-Davidson et al, 2010). Also confocal scanning electron microscopy (SEM) and low-temperature scanning electron microscopy (LTSEM) have been used to study E. necator (Carver et al, 1994; Cadle-Davidson et al, 2010; Gadoury et al, 2012; Ramming et al, 2012; Gao et al, 2016). Stainings successfully employed are: i) Coomassie blue, to monitor conidium germination and infiltration into host cells (Ramming et al, 2011; Ramming et al, 2012), ii) Trypan blue, to label the plant dead cells, following conidium germination, hyphae growth, and conidiophore emergence (Gao et al, 2016); iii) Aniline blue, to follow the infection using bright microscopy (Fekete et al, 2009; Gao et al, 2012a) as well as to localise the spores using fluorescence (Vanacker et al, 2000; Pessina et al, 2016).…”

Section: Disease Descriptionmentioning

confidence: 99%

Emergent Ascomycetes in Viticulture: An Interdisciplinary Overview

et al. 2019

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The reduction of pesticide usage is a current imperative and the implementation of sustainable viticulture is an urgent necessity. A potential solution, which is being increasingly adopted, is offered by the use of grapevine cultivars resistant to its main pathogenic threats. This, however, has contributed to changes in defense strategies resulting in the occurrence of secondary diseases, which were previously controlled. Concomitantly, the ongoing climate crisis is contributing to destabilizing the increasingly dynamic viticultural context. In this review, we explore the available knowledge on three Ascomycetes which are considered emergent and causal agents of powdery mildew, black rot and anthracnose. We also aim to provide a survey on methods for phenotyping disease symptoms in fields, greenhouse and lab conditions, and for disease control underlying the insurgence of pathogen resistance to fungicide. Thus, we discuss fungal genetic variability, highlighting the usage and development of molecular markers and barcoding, coupled with genome sequencing. Moreover, we extensively report on the current knowledge available on grapevine-ascomycete interactions, as well as the mechanisms developed by the host to counteract the attack. Indeed, to better understand these resistance mechanisms, it is relevant to identify pathogen effectors which are involved in the infection process and how grapevine resistance genes function and impact the downstream cascade. Dealing with such a wealth of information on both pathogens and the host, the horizon is now represented by multidisciplinary approaches, combining traditional and innovative methods of cultivation. This will support the translation from theory to practice, in an attempt to understand biology very deeply and manage the spread of these Ascomycetes.

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Identification and utilization of a new Erysiphe necator isolate NAFU1 to quickly evaluate powdery mildew resistance in wild Chinese grapevine species using detached leaves

Cited by 34 publications

References 29 publications

High-throughput sequencing of microbial community diversity in soil, grapes, leaves, grape juice and wine of grapevine from China

High-throughput sequencing of microbial community diversity in soil, grapes, leaves, grape juice and wine of grapevine from China

CRISPR/Cas9-mediated mutagenesis of VvMLO3 results in enhanced resistance to powdery mildew in grapevine (Vitis vinifera)

Emergent Ascomycetes in Viticulture: An Interdisciplinary Overview

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