Effects of Oral Administration of Chitin Nanofiber on Plasma Metabolites and Gut Microorganisms

Abstract

The aim of this study was to examine the effects of oral administration of chitin nanofibers (CNFs) and surface-deacetylated (SDA) CNFs on plasma metabolites using metabolome analysis. Furthermore, we determined the changes in gut microbiota and fecal organic acid concentrations following oral administrations of CNFs and SDACNFs. Healthy female mice (six-week-old) were fed a normal diet and administered tap water with 0.1% (v/v) CNFs or SDACNFs for 28 days. Oral administration of CNFs increased plasma levels of adenosine triphosphate (ATP), adenosine diphosphate (ADP), and serotonin (5-hydroxytryptamine, 5-HT). Oral administration of SDACNFs affected the metabolisms of acyl-carnitines and fatty acids. The fecal organic level analysis indicated that oral administration of CNFs stimulated and activated the functions of microbiota. These results indicate that oral administration of CNFs increases plasma levels of ATP and 5-HT via activation of gut microbiota.

Protein/CaCO3/Chitin Nanofiber Complex Prepared from Crab Shells by Simple Mechanical Treatment and Its Effect on Plant Growth

“…In a previous study, we isolated chitin nanofibers from crab shells and prepared them using a simple mechanical treatment [2]. Chitin nanofibers have a characteristic morphology [3], high surface-to-volume ratio [4], high mechanical strength [5,6], and efficient biological properties [7,8,9,10]. …”

CE–MS-based metabolomics reveals the metabolic profile of maitake mushroom (Grifola frondosa) strains with different cultivation characteristics

“…30,31) In addition, it has been reported that the oral administration of chitin nanofibers activated the functions of gut microorganisms and increased the plasma level of ATP. 32) These functionalities are thus expected to be higher in Gf433, which contains more chitin.…”

Isoxanthohumol, a hop-derived flavonoid, alters the metabolomics profile of mouse feces

“…Such a metabolomics approach can be used to elucidate the mechanism of functional foods. Azuma et al used LC-TOFMS to show that oral administration of chitin nanofiber affects the metabolism of acyl-carnitines and fatty acids, confirming the effectiveness of the technology [33].…”

“…A metabolomics approach using capillary electrophoresis mass spectrometry with time-of-flight (CE-TOFMS) makes it possible to quantify charged low-molecular-weight compounds produced by intestinal microbiota with high resolution and high throughput, providing a detailed explanation for the host-intestinal bacterial interactions at the molecular level [32]. Combined with liquid chromatography mass spectrometry with time-of-flight (LC-TOFMS), which is adequate for relatively lipophilic compounds such as fatty acids and bile acids, various biological compounds can be determined and quantified [33]. Such a metabolomics approach can be used to elucidate the mechanism of functional foods.…”