Antihyperglycemic Activity ofEucalyptus tereticornisin Insulin-Resistant Cells and a Nutritional Model of Diabetic Mice

Abstract

Eucalyptus tereticornis is a plant used in traditional medicine to control diabetes, but this effect has not been proved scientifically. Here, we demonstrated through in vitro assays that E. tereticornis extracts increase glucose uptake and inhibit their production in insulin-resistant C2C12 and HepG2 cells, respectively. Furthermore, in a nutritional model using diabetic mice, the administration of ethyl acetate extract of E. tereticornis reduced fasting glycaemia, improved tolerance to glucose, and reduced resistance to insulin. Likewise, this extract had anti-inflammatory effects in adipose tissue when compared to control diabetic mice. Via bioguided assays and sequential purification of the crude extract, a triterpenoid-rich fraction from ethyl acetate extracts was shown to be responsible for the biological activity. Similarly, we identified the main compound responsible for the antihyperglycemic activity in this extract. This study shows that triterpenes found in E. tereticornis extracts act as hypoglycemic/antidiabetic compounds and contribute to the understanding of their use in traditional medicine.

C2C12 cell model: its role in understanding of insulin resistance at the molecular level and pharmaceutical development at the preclinical stage

“…Eucalyptus tereticornis crude extracts enhanced glucose uptake and inhibited gluconeogenesis in insulin‐resistant C2C12 skeletal muscle and HepG2 liver cells, respectively. [ 190 ] In this study, palmitate treatment caused a 35.7% reduction of glucose uptake in the established insulin‐resistant C2C12 cell model. Under insulin‐resistant condition, crude extract (100 μg/ml) increased glucose uptake by 17.1% as compared to negative control (insulin‐resistant cells), although its antihyperglycaemic effect was lower than metformin treatment (1 m m ).…”

Biological, medicinal and toxicological significance of Eucalyptus leaf essential oil: a review

“…Both α‐amylase and α‐glucosidase were inhibited by a non‐competitive mechanism. Guillén et al . demonstrated through in vitro assays that E. tereticornis extracts increase glucose uptake and inhibit their production in insulin‐resistant C 2 C 12 and HepG 2 cells, respectively.…”

Anti-diabetic activity-guided screening of aqueous-ethanol Moringa oleifera extracts and fractions: Identification of marker compounds

“…We found that the 95 % (v/v) ethanol extract and the butanol fraction thereof were the most active extract and fraction, respectively, exerting very significant acute anti-hyperglycaemic activities in diabetic rats. These effects may be attributable to the expression of glucose transporter-4 (GLUT-4) in the cell membrane, which promotes glucose uptake by skeletal muscle cells and inhibition of glucose synthesis in hepatic cells [18]; skeletal muscle cells constitute 70 % of the total body mass [19]. The results of multiple dosing were in agreement with those of a previous study [20] of the anti-diabetic potential of M. oleifera aqueous ethanol extracts (p < 0.001).…”