Studying antibiotic–membrane interactions via X‐ray diffraction and fluorescence microscopy

Sun, Yiting; Huang, Ping-Yuan; Lin, Cheng-Yu; Lee, Kuan‐Rong; Lee, Ming-Tao

doi:10.1016/j.fob.2015.06.006

Cited by 4 publications

(2 citation statements)

References 55 publications

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“…Because the membrane can be regarded as an incompressible fluid, the area expansion requires a reduction in bilayer thickness to maintain a constant volume. Such membrane thinning induced by surface-aligned amphiphilic peptides has been indeed observed experimentally (Ludtke et al, 1995 ; Staudegger et al, 2000 ; Chen et al, 2003 ; Mecke et al, 2005 ; Jang et al, 2006 ; Kim et al, 2009 ; Sun et al, 2015 ). The opposite response of bilayers to amphiphilic helical peptides, i.e., an increase in bilayer thickness, has also been reported in rare cases.…”

Section: Introductionmentioning

confidence: 59%

Membrane Thinning and Thickening Induced by Membrane-Active Amphipathic Peptides

Grage

Afonin

Kara

et al. 2016

Front. Cell Dev. Biol.

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Membrane thinning has been discussed as a fundamental mechanism by which antimicrobial peptides can perturb cellular membranes. To understand which factors play a role in this process, we compared several amphipathic peptides with different structures, sizes and functions in their influence on the lipid bilayer thickness. PGLa and magainin 2 from X. laevis were studied as typical representatives of antimicrobial cationic amphipathic α-helices. A 1:1 mixture of these peptides, which is known to possess synergistically enhanced activity, allowed us to evaluate whether and how this synergistic interaction correlates with changes in membrane thickness. Other systems investigated here include the α-helical stress-response peptide TisB from E. coli (which forms membrane-spanning dimers), as well as gramicidin S from A. migulanus (a natural antibiotic), and BP100 (designer-made antimicrobial and cell penetrating peptide). The latter two are very short, with a circular β-pleated and a compact α-helical structure, respectively. Solid-state 2H-NMR and grazing incidence small angle X-ray scattering (GISAXS) on oriented phospholipid bilayers were used as complementary techniques to access the hydrophobic thickness as well as the bilayer-bilayer repeat distance including the water layer in between. This way, we found that magainin 2, gramicidin S, and BP100 induced membrane thinning, as expected for amphiphilic peptides residing in the polar/apolar interface of the bilayer. PGLa, on the other hand, decreased the hydrophobic thickness only at very high peptide:lipid ratios, and did not change the bilayer-bilayer repeat distance. TisB even caused an increase in the hydrophobic thickness and repeat distance. When reconstituted as a mixture, PGLa and magainin 2 showed a moderate thinning effect which was less than that of magainin 2 alone, hence their synergistically enhanced activity does not seem to correlate with a modulation of membrane thickness. Overall, the absence of a typical thinning response in the case of PGLa, and the increase in the repeat distance and membrane thickening observed for TisB, demonstrate that the concept of peptide-induced membrane thinning cannot be generalized. Instead, these results suggest that different factors contribute to the resulting changes in membrane thickness, such as the peptide orientation in the bilayer, and/or bilayer adaptation to hydrophobic mismatch.

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Section: Introductionmentioning

confidence: 59%

Membrane Thinning and Thickening Induced by Membrane-Active Amphipathic Peptides

Grage

Afonin

Kara

et al. 2016

Front. Cell Dev. Biol.

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“…Using the time-dependent density function method, it is possible to determine the states of electron transfer at the excited states and to extract the ultraviolet or visible spectrum of a compound. These spectra can also be used to identify compounds and trace them in biological systems [51][52][53]. The excited states and UV-visible spectra for studied complexes at 30 states are computed by cam-B3LYP/6-31G (d, p) level of DFT theory, and results are tabulated in Table 3 and are shown in Fig.…”

Section: Uv-visible Spectrummentioning

confidence: 99%

A quantum assessment of the interaction between Glycine amino acid with pristine and B&N doped silicon carbide nanocage (Si 12 C 12 )

Rezaei–Sameti

Naghibi

2023

Preprint

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Density functional theory is utilized for scrutinizing the interaction of Glycine amino acid (Gly) with the Si12C12, BSi11C12, NSi11C12, BSi12C11, and NSi12C11 nanocages. The adsorption and deformation energy, solvent effect, topological parameters of atom in the molecule (AIM), time-dependent (TD-DFT), reduced density gradient (RDG), natural bonding (NBO), and quantum descriptors for all considered complexes of Gly&SiC nanocages are computed. The adsorption and enthalpy energy of formation all considered Gly&SiC nanocage complexes are exothermic, and interaction of Gly from oxygen site (C = O) with NSi11C12 and BSi12C11 nanocage is more favorable than other complexes. In the presence of water solvent, the formation of Gly&SiC nanocage complexes is non-spontaneous. The NBO charge and electrostatic potential (ESP) results display that the Gly molecule has a donor electron effect on the SiC nanocage surface. The gap energy and hardness property of Gly&SiC nanocage complexes depict that the conductivity and reactivity complex increases. The AIM and RDG parameters confirm that the bonding between Gly and SiC nanocage is partially covalent or electrostatic type. The electrical and optical properties of Gly with BSi11C12, NSi11C12, BSi12C11, and NSi12C11 nanocages change more than Si12C12 nanocages, and these nanocages can be applied as a selective sensor and absorber for Gly.

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Analytical techniques and methods for study of drug-lipid membrane interactions

Zhao

Sun

2017

Reviews in Analytical Chemistry

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A better elucidation of molecular mechanisms underlying drug-membrane interaction is of great importance for drug research and development. To date, different biochemical and biophysical methods have been developed to study biological membranes at molecular level. This review focuses on the recent applications and achievements of modern analytical techniques in the study of drug interactions with lipid membranes, including chromatography, spectrometry, calorimetry, and acoustic sensing. The merits and limitations of these techniques were compared and critically discussed. Moreover, various types of biomimetic model membranes including liposomes, lipid monolayers, and supported lipid monolayers/bilayers were described. General mechanisms underlying drug-membrane interaction process were also briefly introduced.

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Studying antibiotic–membrane interactions via X‐ray diffraction and fluorescence microscopy

Abstract: HighlightsWe present antibiotic-induced membrane thinning of a multi-lamellar thin film sample.Both penicillin and sulbactam are found positioned outside the model membrane in an aqueous solution.We demonstrate a hybrid method to study the antibiotic–membrane interaction.

Cited by 4 publications

References 55 publications

Membrane Thinning and Thickening Induced by Membrane-Active Amphipathic Peptides

Membrane Thinning and Thickening Induced by Membrane-Active Amphipathic Peptides

A quantum assessment of the interaction between Glycine amino acid with pristine and B&N doped silicon carbide nanocage (Si 12 C 12 )

Analytical techniques and methods for study of drug-lipid membrane interactions

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Studying antibiotic–membrane interactions via X‐ray diffraction and fluorescence microscopy

Abstract: HighlightsWe present antibiotic-induced membrane thinning of a multi-lamellar thin film sample.Both penicillin and sulbactam are found positioned outside the model membrane in an aqueous solution.We demonstrate a hybrid method to study the antibiotic–membrane interaction.

Cited by 4 publications

References 55 publications

Membrane Thinning and Thickening Induced by Membrane-Active Amphipathic Peptides

Membrane Thinning and Thickening Induced by Membrane-Active Amphipathic Peptides

A quantum assessment of the interaction between Glycine amino acid with pristine and B&amp;N doped silicon carbide nanocage (Si 12 C 12 )

Analytical techniques and methods for study of drug-lipid membrane interactions

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Product

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A quantum assessment of the interaction between Glycine amino acid with pristine and B&N doped silicon carbide nanocage (Si 12 C 12 )