Analytical techniques and methods for study of drug-lipid membrane interactions

Abstract

A better elucidation of molecular mechanisms underlying drug-membrane interaction is of great importance for drug research and development. To date, different biochemical and biophysical methods have been developed to study biological membranes at molecular level. This review focuses on the recent applications and achievements of modern analytical techniques in the study of drug interactions with lipid membranes, including chromatography, spectrometry, calorimetry, and acoustic sensing. The merits and limitations of these techniques were compared and critically discussed. Moreover, various types of biomimetic model membranes including liposomes, lipid monolayers, and supported lipid monolayers/bilayers were described. General mechanisms underlying drug-membrane interaction process were also briefly introduced.

Interactions of primaquine and chloroquine with PEGylated phosphatidylcholine liposomes

“…The physicochemical characteristics were specifically, evaluated using FTIR spectroscopy, P-XRD, and DTA. These evaluations were performed to analyze the interaction between the drugs and the lipid membrane of the liposomes 32 , 33 .…”

Teicoplanin-Modified HPLC Column as a Source of Experimental Parameters for Prediction of the Anticonvulsant Activity of 1,2,4-Triazole-3-Thiones by the Regression Models

“…Recently, immobilized liposome chromatography (ILC) raises great interest and hopes in simulating membrane-drug interactions. Many researchers emphasize its usefulness for the screening of drug partition in lipid bilayers [17][18][19][20][21]. The potential of this method is associated with the possibility of introducing additional ligands to the liposome surface.…”

Interactions of Cationic Diruthenium Trithiolato Complexes with Phospholipid Membranes Studied by NMR Spectroscopy

“…1D 1 H and 31 P NMR spectroscopy: 1D 1 H NMR spectra were recorded using the zgpr30 (Bruker) pulse sequence for presaturation of the residual water resonance. 1D 31 P NMR spectra were obtained using the zgpg sequence (Bruker) with broadband 1 H decoupling.…”

“…Using NMR spectroscopy, the location of a guest molecule at the vesicle or micelle interface, aggregation, encapsulation and other aspects of drug-membrane interactions can be studied. [23][24][25][26][27][28][29][30][31][32] This study is aiming to provide an insight into the interactions of diruthenium complexes with cell membranes. For this purpose, three representative complexes [(ɳ 6 -p-MeC 6 H 4 Pr i ) 2 Ru 2 (R 1 ) 2 (R 2 )] + with different degrees of lipophilicity and in vitro cytotoxicity 7 (R 1,2 = SC 6 H 4 -oi Pr: 1; R 1 = SC 6 H 4 -p-OMe; R 2 = SC 6 H 4 -p-OH : 2; R 1 = SCH 2 C 6 H 4 -OMe; R 2 = SC 6 H 4 -p-OH : 3) were selected (Figure 1) and their interactions towards the membrane models 2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) vesicles and 1,2dihexanoyl-sn-glycero-3-phosphocholine (DHPC) micelles were studied with NMR spectroscopy as main tool.…”