Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: The Joint Danube Survey 3

Kolarević, Stoimir; Kračun-Kolarević, Margareta; Kostić, Jovana; Slobodnı́k, Jaroslav; Liška, Igor; Gačić, Zoran; Paunović, Momír; Knežević-Vukčević, Jelena; Vuković-Gačić, Branka

doi:10.1016/j.scitotenv.2015.06.061

Cited by 47 publications

(22 citation statements)

References 38 publications

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“…This can be explained by a lot of boat careening (dirt-resistance paint against biofouling) and a strong recreational boating activity. Indeed, several studies have shown that dredging or sediment suspension in harbour areas has affected aquatic fauna and the marine ecosystem by organic and inorganic contamination (Steger and Gardner, 2007;Magni et al, 2008;Jane et al, 2010;Sundstein et al, 2010;Kolarević et al, 2016;Chan et al, 2016). Taken together, results from previous reports and in our study show significant anthropogenic impacts on the coastal environment of La Rochelle.…”

Section: Trace Elementssupporting

confidence: 81%

First data on three bivalve species exposed to an intra-harbour polymetallic contamination (La Rochelle, France)

Breitwieser

Viricel

Churlaud

et al. 2017

Comparative Biochemistry and Physiology Part C: Toxicology &

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Evaluating diffuse sediment contamination in the environment is a major concern with the aim of reaching a good chemical and ecological state of the littoral zone. In this study the risks of chronic chemical contamination and consequences in the bivalves Crassostrea gigas, Mytilus sp. and Mimachlamys varia were evaluated in coastal environments. The objective here was to understand the anthropological phenomena that affect the functioning of the marina of La Rochelle (semi-closed environment). Harbours seeking ecomanagement accreditations (such as the international reference ISO 14001) constitute zones of interest to implement biomonitoring studies. The biological effects of chemical pollution in the Marina of La Rochelle were studied to develop a multi-biomarker biomonitoring approach on specific marine species of this site. Moreover, a genetic (DNA barcoding) approach was applied to validate the species identity of collected bivalves. Of the three species tested the scallop, M. varia, was the most sensitive to metal exposure.

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Section: Trace Elementssupporting

confidence: 81%

First data on three bivalve species exposed to an intra-harbour polymetallic contamination (La Rochelle, France)

Breitwieser

Viricel

Churlaud

et al. 2017

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…The level of genotoxic pollution along the Danube River done by measuring the level of DNA damage in the haemocytes of freshwater mussels of Unio sp. (Unio pictorum/Unio tumidus) and Sinanodonta woodiana by Kolarevic et al, (2016). The research was performed on 34 out of 68 sites analysed within the joint survey undertaken in the phase 3 of activities related to Danube.…”

mentioning

confidence: 99%

Effects of Pollution on Freshwater Organisms

Choudri

Baawain²

2017

Water Environment Research

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This review covers research papers and scientific literature published in the year 2016 on the effects of anthropogenic pollutants on freshwater organisms. In the first, the review begins with two broad sections thate are mainly focused on research reviews and broad field studies and surveys. This is followed by reviews of research categorized in sections to reflect the pollutant class. These sections include wastewater, stormwater and non-point source pollution, nutrients, sediment cap materials and suspended clays, botanical extracts, surfactants, metals, persistent organic pollutants, pharmaceuticals, endocrine disruptors, pesticides, petroleum hydrocarbons and polycyclic aromatic hydrocarbons (PAHs), ionic liquids, and nanomaterials. The final section of the review highlights the research published on describing innovations in the field of freshwater pollution research.

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“…In freshwater environments, the zebra mussel (Dreissena polymorpha) is the most common bivalve for genotoxicity assessments using the comet assay [134][135][136][137][138][139][140][141]. Several other freshwater species are also used, such as the quagga mussel (Dreissena bugensis) [137], painter's mussel (Unio pictorum) [142][143][144][145][146], swollen river mussel (Unio tumidus) [142,143,145,147,148], freshwater mussel (Unio tigridis) [149], golden mussel (Limnoperna fortunei) [150,151], Chinese pond mussel (Sinanodonta woodiana) [143,152], Asian clam A c c e p t e d M a n u s c r i p t 10 (Corbicula fluminea) [153][154][155], Lamellidens marginalis [156] and paper pondshell (Utterbackia imbecillis) [157].…”

Section: Bivalvesmentioning

confidence: 99%

The comet assay in animal models: From bugs to whales – (Part 1 Invertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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The comet assay, also called single cell gel electrophoresis, is a sensitive, rapid and low-cost technique for quantifying and analysing DNA damage and repair at the level of individual cells. The assay itself can be applied on virtually any cell type derived from different organs and tissues of eukaryotic organisms. Although it is mainly used on human cells, the assay has applications also in the evaluation of DNA damage in yeast, plant and animal cells. Therefore, the purpose of this review is to give an extensive overview on the usage of the comet assay in animal models from invertebrates to vertebrates, covering both terrestrial and water biota. The comet assay is used in a variety of invertebrate species since they are regarded as interesting subjects in ecotoxicological research due to their significance in ecosystems. Hence, the first part of the review (Part 1) will discuss the application of the comet assay in invertebrates covering protozoans, platyhelminthes, planarians, cnidarians, molluscs, annelids, arthropods and echinoderms. Besides a large number of animal species, the assay is also performed on a variety of cells, which includes haemolymph, gills, digestive gland, sperm and embryo cells. The mentioned cells have been used for the evaluation of a broad spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of invertebrate models and their role from an ecotoxicological point of view will also be discussed as well as the comparison of the use of the comet assay in invertebrate and human models. Since the comet assay is still developing, its increasing potential in assessing DNA damage in animal models is crucial especially in the field of ecotoxicology and biomonitoring at the level of different species, not only humans.

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Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: The Joint Danube Survey 3

Cited by 47 publications

References 38 publications

First data on three bivalve species exposed to an intra-harbour polymetallic contamination (La Rochelle, France)

First data on three bivalve species exposed to an intra-harbour polymetallic contamination (La Rochelle, France)

Effects of Pollution on Freshwater Organisms

The comet assay in animal models: From bugs to whales – (Part 1 Invertebrates)

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