Etoposide Incorporated into Camel Milk Phospholipids Liposomes Shows Increased Activity against Fibrosarcoma in a Mouse Model

Maswadeh, Hamzah; Aljarbou, Ahmad N.; Alorainy, Mohammed S; Alsharidah, Mansour; Khan, Masood Alam

doi:10.1155/2015/743051

Cited by 18 publications

(20 citation statements)

References 31 publications

(23 reference statements)

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“…28 Lip from DPPC or CML mixture containing Dox or ETP were prepared. The pH-gradient method for Dox encapsulation was used.…”

Section: Methodsmentioning

confidence: 99%

“…27 The characterization of the phospholipids isolated from camel milk predominantly showed the presence of phosphatidylcoline, phosphatidylethanolamine (PE), phosphatidylinositol, and lysophosphatidylcholine. 28 Recently we showed that etoposide (ETP) encapsulated into camel milk phospholipids (CML)-Lip possesses greater activity against fibrosarcoma in a mouse model. 28 Cisplatin showed increased antitumor efficacy in PE-containing Lip.…”

mentioning

confidence: 99%

“…28 Recently we showed that etoposide (ETP) encapsulated into camel milk phospholipids (CML)-Lip possesses greater activity against fibrosarcoma in a mouse model. 28 Cisplatin showed increased antitumor efficacy in PE-containing Lip. 29 ETP encapsulated in immunomodulator tuftsin-bearing Lip showed increased antitumor activity.…”

mentioning

confidence: 99%

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Coadministration of doxorubicin and etoposide loaded in camel milk phospholipids liposomes showed increased antitumor activity in a murine model

Maswadeh

Aljarbou

Alorainy

et al. 2015

IJN

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Small unilamellar vesicles from camel milk phospholipids (CML) mixture or from 1,2 dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) were prepared, and anticancer drugs doxorubicin (Dox) or etoposide (ETP) were loaded. Liposomal formulations were used against fibrosarcoma in a murine model. Results showed a very high percentage of Dox encapsulation (~98%) in liposomes (Lip) prepared from CML-Lip or DPPC-Lip, whereas the percentage of encapsulations of ETP was on the lower side, 22% of CML-Lip and 18% for DPPC-Lip. Differential scanning calorimetry curves show that Dox enhances the lamellar formation in CML-Lip, whereas ETP enhances the nonlamellar formation. Differential scanning calorimetry curves also showed that the presence of Dox and ETP together into DPPC-Lip produced the interdigitation effect. The in vivo anticancer activity of liposomal formulations of Dox or ETP or a combination of both was assessed against benzopyrene (BAP)-induced fibrosarcoma in a murine model. Tumor-bearing mice treated with a combination of Dox and ETP loaded into CML-Lip showed increased survival and reduced tumor growth compared to other groups, including the combination of Dox and ETP in DPPC-Lip. Fibrosarcoma-bearing mice treated with a combination of free (Dox + ETP) showed much higher tumor growth compared to those groups treated with CML-Lip-(Dox + ETP) or DPPC-Lip-(Dox + ETP). Immunohistochemical study was also performed to show the expression of tumor-suppressor PTEN, and it was found that the tumor tissues from the group of mice treated with a combination of free (Dox + ETP) showed greater loss of cytoplasmic PTEN than tumor tissues obtained from the groups of mice treated with CML-Lip-(Dox + ETP) or DPPC-Lip-(Dox + ETP).

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“…28 Lip from DPPC or CML mixture containing Dox or ETP were prepared. The pH-gradient method for Dox encapsulation was used.…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Coadministration of doxorubicin and etoposide loaded in camel milk phospholipids liposomes showed increased antitumor activity in a murine model

Maswadeh

Aljarbou

Alorainy

et al. 2015

IJN

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“…In vitro [110] PMF (Cesium (Cs) and Rubidium (Rb) nanoparticles) [132][133][134]. Liposomes prepared from these phospholipids were used as drug delivery systems for various anticancer drugs, and have shown promising anticancer activity in the murine/mouse model.…”

Section: Development Efforts On Camel Products Based Anticancer Nanopmentioning

confidence: 99%

“…Liposomes prepared from these phospholipids were used as drug delivery systems for various anticancer drugs, and have shown promising anticancer activity in the murine/mouse model. Etoposide entrapped in camel milk phospholipid liposomes has showed greater anticancer activity against fibrosarcoma in a murine model compared to free etoposide or etoposide entrapped in 1,2-Dipalmitoyl-snglycero-3-phosphatidylcholine (DPPC) liposomes [132]. Combination of doxorubicin (Dox) and etoposide (ETP) loaded into camel milk phospholipids liposomes (CML-Lip) is found also to increase survival and to reduce tumor growth in the mice than the combination of Dox and ETP in DPPC-Lip [133].…”

Section: Development Efforts On Camel Products Based Anticancer Nanopmentioning

confidence: 99%

Therapeutic Applications of Camel’s Milk and Urine against Cancer: Current Development Efforts and Future Perspectives

Alebie¹,

Yohannes²,

Worku³

2017

J Cancer Sci Ther

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Efficacy of targeted liposomes and nanocochleates containing imatinib plus dexketoprofen against fibrosarcoma

Çoban

Değim

Yılmaz³

et al. 2019

Drug Development Research

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The main challenges in treating cancer using chemotherapeutics are insufficient dose at the target site and the development of drug resistance, while higher doses can induce side effects by damaging nontarget tissues. Combinatorial drug therapy may overcome these limitations by permitting lower doses and more specific targeting, thereby mitigating drug resistance and nontarget side effects. Recent reports indicate that nonsteroidal anti-inflammatory drugs (NSAIDs) have anticancer potential and can be used together with conventional chemotherapeutics to improve efficacy and safety. In the present study, imatinib mesylate and dexketoprofen trometamol were selected as model drugs to develop targeted surface-modified liposome and nanocochleate formulations for fibrosarcoma treatment. The physicochemical properties and in vitro efficacy of various formulations were evaluated by measurement of particle size distribution, polydispersity index, zeta potential, encapsulation efficiency, diffusion through Caco-2 cells, and toxicity in culture. Selected formulations were then evaluated in fibrosarcoma-bearing model mice by histopathological observations and tyrosine kinase receptor inhibition assays.The most effective formulation on the fibrosarcoma model was a PEGylated nanocochleate formulation. These findings provide a foundation for developing more effective formulations and chemotherapeutic strategies for the treatment of fibrosarcoma and other types of cancer.

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Etoposide Incorporated into Camel Milk Phospholipids Liposomes Shows Increased Activity against Fibrosarcoma in a Mouse Model

Cited by 18 publications

References 31 publications

Coadministration of doxorubicin and etoposide loaded in camel milk phospholipids liposomes showed increased antitumor activity in a murine model

Coadministration of doxorubicin and etoposide loaded in camel milk phospholipids liposomes showed increased antitumor activity in a murine model

Therapeutic Applications of Camel’s Milk and Urine against Cancer: Current Development Efforts and Future Perspectives

Efficacy of targeted liposomes and nanocochleates containing imatinib plus dexketoprofen against fibrosarcoma

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