Complete genome sequence of canine astrovirus with molecular and epidemiological characterisation of UK strains

Caddy, Sarah; Goodfellow, Ian

doi:10.1016/j.vetmic.2015.03.011

Cited by 29 publications

(48 citation statements)

References 24 publications

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“…Five strains were grouped with other canine AstVs identified worldwide, but further separated into multiple capsid (ORF2) sub-clusters. By parallelism with what is observed in human AstVs (MAstV species 1), that are classified antigenically in 8 serotypes, it is possible to speculate that the genetic heterogeneity observed in canine AstVs might account for a significant antigenic diversity (Caddy and Goodfellow, 2015). Weak antigenic cross-reactivity has been observed in immune electron microscopy between strain ITA/2008/Bari and ITA/2010/Zoid virus particles and the sera of convalescent dogs (Martella et al, 2012).…”

Section: Discussionmentioning

confidence: 55%

“…Thus, our prevalence data for AstV must be interpreted with caution, even if the detection rates of AstVs fell in the ranges reported in the literature. For example, canine AstV RNA has been detected in 6-27% of dogs with enteritis and 0-19% of aysmptomatic dogs in studies in Italy ( (Takano et al, 2015), France (Grellet et al, 2012) and UK (Caddy and Goodfellow, 2015). Even if those studies differed in the number, provenience and age of the animals enrolled in the investigations, in most studies the detection rates were found to differ significantly between symptomatic and asymptomatic dogs, suggesting a possible role of AstVs as enteric pathogens of dogs.…”

Section: Discussionmentioning

confidence: 99%

“…Also, a canine AstV, strain ITA/08/Bari, was successfully adapted to replicate in vitro on MDCK cells, and AstV-specific antibodies were detected in convalescent canine sera (Martella et al, 2011a(Martella et al, ,b, 2012. The prevalence of AstV infection seems higher in dogs with enteric disease than in asymptomatic animals (Martella et al, 2011a,b;Zhu et al, 2011;Caddy and Goodfellow, 2015;Takano et al, 2015). Also, monitoring of natural infection by AstV in dogs has revealed that the acute phase of gastroenteritis overlaps with peaks of viral shedding (Martella et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…At present, limited and partial information on canine AstV genomes is available in GenBank. This limited amount of information seems to suggest that canine AstVs are genetically heterogeneous (Martella et al, 2011a,b;Caddy and Goodfellow, 2015), thus posing a challenge for the diagnostic and for the understanding of the genetic and biological properties of these viruses in dogs.…”

Section: Introductionmentioning

confidence: 99%

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Genome analysis of canine astroviruses reveals genetic heterogeneity and suggests possible inter-species transmission

et al. 2017

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Canine astrovirus RNA was detected in the stools of 17/63 (26.9%) samples, using either a broadly reactive consensus RT-PCR for astroviruses or random RT-PCR coupled with massive deep sequencing. The complete or nearly complete genome sequence of five canine astroviruses was reconstructed that allowed mapping the genome organization and to investigate the genetic diversity of these viruses. The genome was about 6.6kb in length and contained three open reading frames (ORFs) flanked by a 5' UTR, and a 3' UTR plus a poly-A tail. ORF1a and ORF1b overlapped by 43 nucleotides while the ORF2 overlapped by 8 nucleotides with the 3' end of ORF1b. Upon genome comparison, four strains (HUN/2012/2, HUN/2012/6, HUN/2012/115, and HUN/2012/135) were more related genetically to each other and to UK canine astroviruses (88-96% nt identity), whilst strain HUN/2012/126 was more divergent (75-76% nt identity). In the ORF1b and ORF2, strains HUN/2012/2, HUN/2012/6, and HUN/2012/135 were related genetically to other canine astroviruses identified formerly in Europe and China, whereas strain HUN/2012/126 was related genetically to a divergent canine astrovirus strain, ITA/2010/Zoid. For one canine astrovirus, HUN/2012/8, only a 3.2kb portion of the genome, at the 3' end, could be determined. Interestingly, this strain possessed unique genetic signatures (including a longer ORF1b/ORF2 overlap and a longer 3'UTR) and it was divergent in both ORF1b and ORF2 from all other canine astroviruses, with the highest nucleotide sequence identity (68% and 63%, respectively) to a mink astrovirus, thus suggesting a possible event of interspecies transmission. The genetic heterogeneity of canine astroviruses may pose a challenge for the diagnostics and for future prophylaxis strategies.

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Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome analysis of canine astroviruses reveals genetic heterogeneity and suggests possible inter-species transmission

et al. 2017

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“…To date, MAstV5 was reported only in samples derived from the gastrointestinal tract of dogs [24,25,34,[26][27][28][29][30][31][32][33], and the recovery of MAstV5 in the crab-eating fox's CNS is an interesting and unexpected finding. However, the detection of other AstV species in the central nervous system (CNS) of mink [35], cattle [36,37], human [38], pigs [39] and sheep [40] has been the focus of differential diagnosis of nonsuppurative encephalitis.…”

Section: Detection Of Common Canine Enteric Virusesmentioning

confidence: 99%

Mamastrovirus 5 detected in a crab-eating fox (Cerdocyon thous): Expanding wildlife host range of astroviruses

Alves

Budaszewski

Cibulski

et al. 2018

Comparative Immunology, Microbiology and Infectious Diseases

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Astroviruses are a common cause of gastroenteritis in children worldwide and can also cause infection in a range of domestic and wild animal species. Canine astrovirus (formally named as Mamastrovirus 5, MAstV5) has been reported worldwide, and its role as an enteric pathogen is still controversial. Herein, we describe the genomic characterization of a MAstV5 (strain crab-eating fox/2016/BRA) identified in a wild canid (Cerdocyon thous) diagnosed with canine distemper virus (CDV) as causa mortis. The nearly complete genome comprised 6579 nt in length and displayed the archetypal organization of astroviruses. The present report is the first evidence of MAstV5 infection in an animal species other than the dog and highlights a possible natural astrovirus spillover between domestic and wild canids. Moreover, these results show the first evidence of extra-intestinal MAstV5, suggesting a virus systemic spread. This work is expected to contribute to a better understanding of the astroviruses biology and their interactions with the wildlife health.

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A comprehensive molecular survey of viral pathogens associated with canine gastroenteritis

et al. 2023

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Viral pathogens are the primary cause of canine gastroenteritis. However, few structured comprehensive studies on the viral etiology of canine gastroenteritis have been conducted. In this study, 475 rectal swabs collected over three years (2018-2021) from clinical canine gastroenteritis cases were screened for the presence of six major enteric viruses – canine parvovirus 2 (CPV-2), canine distemper virus (CDV), canine adenovirus 2 (CAdV-2), canine coronavirus (CCoV), canine astrovirus (CaAstV), and canine rotavirus (CRV) – by real-time PCR. The most frequently detected virus was CPV-2, which was present in 64.8% of the samples (subtype 2a, 21.1%; 2b, 77.4%; 2c, 1.5%), followed by CDV (8%), CaAstV (7.2%), CCoV (5.9%), and CAdV-2 (4.6%). Two to four of these viruses in different combinations were found in 16.8% of the samples, and CRV was not detected. The complete genome sequences of Indian isolates of CDV, CCoV, and CaAstV were determined for the first time, and phylogenetic analysis was performed. This study highlights the need for routine prophylactic vaccination with the appropriate vaccines. Notably, 70.3% of animals vaccinated with DHPPiL were found to be positive for at least one virus. Hence, regular molecular analysis of the prevalent viruses is crucial for addressing vaccination failures. Supplementary Information The online version contains supplementary material available at 10.1007/s00705-022-05674-6.

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Complete genome sequence of canine astrovirus with molecular and epidemiological characterisation of UK strains

Cited by 29 publications

References 24 publications

Genome analysis of canine astroviruses reveals genetic heterogeneity and suggests possible inter-species transmission

Genome analysis of canine astroviruses reveals genetic heterogeneity and suggests possible inter-species transmission

Mamastrovirus 5 detected in a crab-eating fox (Cerdocyon thous): Expanding wildlife host range of astroviruses

A comprehensive molecular survey of viral pathogens associated with canine gastroenteritis

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