Anti‐senescence effect of FatI gene in goat somatic cells

Abstract

The fatty acid dehydrogenase I (FatI) is able to express in mammalian cells and convert n-6 polyunsaturated fatty acids (PUFAs) to n-3 PUFAs. n-3 PUFA is an important component of the cell membrane and plays an important role in the prevention and control of a variety of human diseases. However, n-3 PUFAs cannot be endogenously synthesized by mammals because they lack the dehydrogenase that converts n-6 to n-3 PUFA. For the time being, gradually matured transgenic technology makes it possible to produce transgenic animals that are able to synthesize n-3 PUFAs by themselves. However, the transgenic technology itself may bring negative impacts. In this study, the eukaryotic expression vector pcDNA3.1-FatI was introduced into the genome of Boer goat fetal fibroblasts cultured in vitro, and the influence of biological characteristics of the fetal fibroblast was studied via overexpression of FatI. The results showed that the proliferation and apoptosis of cultured fetal fibroblast were not affected significantly by the overexpression of FatI using BrdU and TUNEL staining methods, respectively. Moreover, the overexpression of FatI significantly inhibited the senescence of somatic cells compared with enhanced green fluorescent protein (EGFP) transgenic cells (P < 0.01). Quantitative PCR revealed that the mRNA expression of P16 and P53 in the FatI transgenic cell group was significantly lower than that in the EGFP transgenic cell group (P < 0.01). In conclusion, the senescence of goat somatic cells was inhibited by the overexpression of the FatI gene.

Polyunsaturated fatty acids ameliorate aging via redox-telomere-antioncogene axis

“…Such balance is also applicable when n-3 PUFA are used to manage antioncogenes. A recent study indicated the anti-aging potential of n-3 PUFA that the overexpression of Fat1 , an endogenously synthesizing n-3 PUFA model, in Boer goat fetal fibroblasts reduced the mRNA expression of p16 and p53 [49]. Nevertheless, fish oil mediates apoptosis of tumor cells by promoting the protein expression of p53 [50].…”

The use of genetic engineering techniques to improve the lipid composition in meat, milk and fish products: a review

“…The results of a subsequent study by these authors indicated that there is no possibility of integration and insertion of the foreign fat-1 gene in transgenic pigs into the genome of wild nontransgenic pigs by suckling or mating . Recently Sun et al (2014) reported that the proliferation and apoptosis of Boer-goat-cultured foetal fibroblasts were not affected, while the senescence of goat somatic cells was inhibited, by overexpression of the fat-1 transgene.…”