Immunohistochemical expression of fibrillin‐1 and fibrillin‐2 during tooth development

Kira-Tatsuoka, Michiko; Oka, Kyoko; Tsuruga, Eichi; Ozaki, Masao; Sawa, Yoshihiko

doi:10.1111/jre.12256

Cited by 7 publications

(4 citation statements)

References 35 publications

(48 reference statements)

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“…Though TGF‐β is ubiquitously present in tissues, its activity is altered by extracellular matrix components, exosomes, and soluble factors that regulate several transcription factors known as EMT/MET transcription factors. During root development, fibrillin fibers regulating TGF‐β signaling are formed in the process of periodontal tissue formation among fragmented HERS 23 ; consequently, TGF‐β activity may also be downregulated. The unique cellular dynamics of HERS is controlled by a fine‐tuned p‐EMT regulatory mechanism.…”

Section: Discussionmentioning

confidence: 99%

Semaphorin‐RhoA signaling regulates HERS maintenance by acting against TGF‐β‐induced EMT

Azumane

Ikezaki

Otsu

et al. 2022

J of Periodontal Research

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Background and Objectives Hertwig's epithelial root sheath (HERS) plays a role in root dentin formation. It produces the epithelial rests of Malassez (ERM) for the induction of periodontal tissue development during root formation. Although ERM is thought to be caused by epithelial–mesenchymal transition (EMT), the mechanism by which HERS is maintained as epithelium is unknown. Here, we aimed to elucidate the molecular mechanisms regulating the relationship between HERS maintenance and ERM development. Methods To understand the relationship between HERS and ERM development during root formation, we observed the developing molar root using cytokeratin14 (CK14) Cre/tdTomato mice via stereomicroscopy. The relationship between semaphorin and transforming growth factor (TGF) signaling in the maintenance of HERS and ERM development was examined using CK14cre/R26‐tdTomato mice and a HERS cell line. Results tdTomato‐positive cells were observed on HERS and the migrating cells from HERS. The migrating cells showed reduced E‐cadherin expression. In contrast, HERS cells expressed semaphorin receptors and active RhoA. Semaphorin signaling was associated with RhoA activation and cell–cell adhesion, while TGF‐β induced decreased E‐cadherin and active RhoA expression, and consequently enhanced cell migration. Conclusion HERS induces root formation by controlling epithelial maintenance and EMT through the opposing effects of semaphorin and TGF‐β signaling.

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Section: Discussionmentioning

confidence: 99%

Semaphorin‐RhoA signaling regulates HERS maintenance by acting against TGF‐β‐induced EMT

Azumane

Ikezaki

Otsu

et al. 2022

J of Periodontal Research

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“…In the future, additional approaches including DE stem cell lineage tracing, stem cell transplantation assays, and the expression of additional EK markers such as p21, BMP, and Wnt will be examined to further validate these findings. Interestingly, bioengineered tooth buds also expressed tooth-specific ECM markers Fib1 and Fib2, which in natural teeth are expressed in later stage DE and early stage DM (Kira-Tatsuoka et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Bioengineered Tooth Buds Exhibit Features of Natural Tooth Buds

et al. 2018

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Tooth loss is a significant health issue currently affecting millions of people worldwide. Artificial dental implants, the current gold standard tooth replacement therapy, do not exhibit many properties of natural teeth and can be associated with complications leading to implant failure. Here we propose bioengineered tooth buds as a superior alternative tooth replacement therapy. We describe improved methods to create highly cellularized bioengineered tooth bud constructs that formed hallmark features that resemble natural tooth buds such as the dental epithelial stem cell niche, enamel knot signaling centers, transient amplifying cells, and mineralized dental tissue formation. These constructs were composed of postnatal dental cells encapsulated within a hydrogel material that were implanted subcutaneously into immunocompromised rats. To our knowledge, this is the first report describing the use of postnatal dental cells to create bioengineered tooth buds that exhibit evidence of these features of natural tooth development. We propose future bioengineered tooth buds as a promising, clinically relevant tooth replacement therapy.

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“…Fibrillin‐1 and fibrillin‐2 have high homology with regard to overall amino acid components (Lin et al, 2002). To further examine the mechanism of oxytalan fiber formation, we investigated the fibrillin 1 and fibrillin 2 expression in vivo during tooth development in mice and humans by immunohistochemistry (Kira‐Tatsuoka, Oka, Tsuruga, Ozaki, & Sawa, 2015). In mice, the expression fibrillin‐1 with a fibrotic shape always starts from the root surface between fragmented epithelial cells derived from Hertwig's epithelial root sheath (HERS) during the root‐forming stage (Figure 5).…”

Section: Development Of Oxytalan Fibers In Pdl Formationmentioning

confidence: 99%

Fibrillin protein, a candidate for creating a suitable scaffold in PDL regeneration while avoiding ankylosis

Oka

2022

Genesis

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Summary The tooth is stabilized by fiber‐rich tissue called the periodontal ligament (PDL). The narrow space of the PDL does not calcify in the physiological state even thought it exists between two calcified tissues, namely, the cementum of the root and alveolar bone. Two situations that require PDL regeneration are periodontitis and dental trauma. Periodontitis induces the loss of PDL and alveolar bone due to inflammation related to infection. Conversely, in PDLs damaged by dental trauma, accelerating bone formation as an overreaction of the healing process is induced, thereby inducing dentoalveolar ankylosis at the tooth root surface. PDL regeneration following dental trauma must therefore be considered separately from periodontitis. Therefore, PDL regeneration in dental trauma must be considered separately from periodontitis. This review focuses on the components involved in avoiding dentoalveolar ankylosis, including oxytalan fibers, aggregated microfibrils, epithelial cell rests of Malassez (ERM), and TGF‐β signaling. During root development, oxytalan fibers produced by PDL cells work in collaboration with the epithelial components in the PDL (e.g., Hertwig's root sheath [HERS] and ERM). We herein describe the functions of oxytalan fibers, ERM, and TGF‐β signals which are involved in the avoidance of bone formation.

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Immunohistochemical expression of fibrillin‐1 and fibrillin‐2 during tooth development

Cited by 7 publications

References 35 publications

Semaphorin‐RhoA signaling regulates HERS maintenance by acting against TGF‐β‐induced EMT

Semaphorin‐RhoA signaling regulates HERS maintenance by acting against TGF‐β‐induced EMT

Bioengineered Tooth Buds Exhibit Features of Natural Tooth Buds

Fibrillin protein, a candidate for creating a suitable scaffold in PDL regeneration while avoiding ankylosis

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