Comparison of urine and cervical samples for detecting human papillomavirus (HPV) with the Cobas 4800 HPV test

Bernal, Samuel; Palomares, J.C.; Artura, Antonio; Parra, Manuel; Cabezas, José Luis; Robles, Antonio Gianluca; Martín-Mazuelos, Estrella

doi:10.1016/j.jcv.2014.10.001

Cited by 37 publications

(58 citation statements)

References 17 publications

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“…In our study, the agreement between any HPV type detection in urine and cervical samples was 84.00% with a Cohen's kappa coefficient of 0.60, similar to that reported by Cuschieri and colleagues. In relation to concordance for LR-HPV and HR-HPV types included in tetravalent vaccine, in our study, concordance was substantial for both, k ¼ 0.70 (95% CI, 0.66-0.74) for HPV-6 and -11 and k ¼ 0.76 (95% CI, 0.78-0.84) for HPV-16 and -18 (31,37,41,(43)(44)(45).…”

Section: Discussionmentioning

confidence: 52%

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Comparison between Urine and Cervical Samples for HPV DNA Detection and Typing in Young Women in Colombia

Cómbita

Gheit

González

et al. 2016

Cancer Prevention Research

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Urine sampling for HPV DNA detection has been proposed as an effective method for monitoring the impact of HPV vaccination programs; however, conflicting results have been reported. The goal of this study was to evaluate the performance of optimized urine HPV DNA testing in women aged 19 to 25 years. Optimization process included the use of first void urine, immediate mixing of urine with DNA preservative, and the concentration of all HPV DNA, including cell-free DNA fragments. Urine and cervical samples were collected from 535 young women attending cervical screening at health centers from two Colombian cities. HPV DNA detection and genotyping was performed using an HPV type-specific multiplex genotyping assay, which combines multiplex polymerase chain reaction with bead-based Luminex technology. Concordance between HPV DNA detection in urine and cervical samples was determined using kappa statistics and McNemar tests. The accuracy of HPV DNA testing in urine samples was evaluated measuring sensitivity and specificity using as reference the results obtained from cervical samples. Statistical analysis was performed using STATA11.2 software. The findings revealed an overall HPV prevalence of 60.00% in cervical samples and 64.72% in urine samples, HPV-16 being the most frequent HPV type detected in both specimens. Moreover, our results indicate that detection of HPV DNA in first void urine provides similar results to those obtained with cervical samples and can be used to monitor HPV vaccination trials and programs as evidenced by the substantial concordance found for the detection of the four vaccine types.Cancer Prev Res; 9(9); 766-71. Ó2016 AACR.

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Section: Discussionmentioning

confidence: 52%

“…31), Bernal and colleagues (53% vs. 50%; ref. 41), and Ducancelle and colleagues (59% vs. 54%; ref. 31), among women between 18 and 25 years old.…”

Section: Discussionmentioning

confidence: 94%

Comparison between Urine and Cervical Samples for HPV DNA Detection and Typing in Young Women in Colombia

Cómbita

Gheit

González

et al. 2016

Cancer Prevention Research

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“…HPV detection in urine samples was usually compared with HPV positivity in cervical samples with or without cytological correlation. However, only a few studies have evaluated the use of clinically validated high-risk HPV tests in urine samples in the detection of histologic HSIL+ [5, 7–9]. …”

Section: Discussionmentioning

confidence: 99%

“…The applications of validated high-risk HPV testing in urine samples from women with abnormal cytology have been reported in 3 previous studies [5, 7, 9]. Sellors et al [5] compared HPV detection using Hybrid Capture 2 between cervical samples, vulvar samples, vaginal swab samples, and urine samples (the latter three sample types being self-collected) from 200 women.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of Human Papillomavirus Detection in Urine and Cervical Samples Using High-Risk HPV DNA Testing in Northern Thailand

Khunamornpong

Settakorn

Sukpan

et al. 2016

Obstetrics and Gynecology International

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Objective. To evaluate the performance of high-risk human papillomavirus (HPV) DNA testing in urine samples compared to that of cervical sample testing in Northern Thailand. Methods. Paired urine and cervical samples were collected during the follow-up of women with a previous positive HPV test. HPV testing was performed using the Cobas 4800 HPV Test. Linear Array assay was used for genotyping in selected cases. Results. Paired urine and cervical samples were obtained from 168 women. Of 123 paired samples with valid results, agreement in the detection of high-risk HPV DNA was present in 106 cases (86.2%), with a kappa statistic of 0.65 (substantial agreement). Using the cervical HPV results as a reference, the sensitivity of urine HPV testing was 68.6% (24/35) and the specificity 93.2% (82/88). For the detection of histologic high-grade squamous intraepithelial lesion or worse (HSIL+), the sensitivity of urine HPV testing was 80.0% (4/5) and the specificity 78.0% (92/118). Conclusion. Although urine HPV testing had a rather low sensitivity for HPV detection, its sensitivity for histologic HSIL+ detection was high. For clinical use of urine HPV testing, standardization of specimen collection and processing techniques or application of a more sensitive test, especially in the detection of HPV52 and HPV58, is necessary.

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Urine test for HPV genotypes as a predictor of precancerous cervical lesions and for cervical cancer screening

Maged

Saad

Salah

et al. 2018

Intl J Gynecology & Obste

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Comparison of urine and cervical samples for detecting human papillomavirus (HPV) with the Cobas 4800 HPV test

Cited by 37 publications

References 17 publications

Comparison between Urine and Cervical Samples for HPV DNA Detection and Typing in Young Women in Colombia

Comparison between Urine and Cervical Samples for HPV DNA Detection and Typing in Young Women in Colombia

Comparison of Human Papillomavirus Detection in Urine and Cervical Samples Using High-Risk HPV DNA Testing in Northern Thailand

Urine test for HPV genotypes as a predictor of precancerous cervical lesions and for cervical cancer screening

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