Urine test for <scp>HPV</scp> genotypes as a predictor of precancerous cervical lesions and for cervical cancer screening

Maged, Ahmed M.; Saad, Hany; Salah, Eman; Meshaal, Hadeer; Abdelbar, Mostafa F.; Omran, Eman; Eldaly, Ashraf

doi:10.1002/ijgo.12453

Cited by 9 publications

(7 citation statements)

References 21 publications

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“…Urine sampling may present an even less invasive and equally effective strategy for primary HPV testing [ [23] , [24] , [25] , [26] , [27] , [28] ], but has not yet been validated with the Xpert HPV assay. Beyond DNA testing, methylation changes in human genes and the HPV virus are strongly correlated with the development of CIN and invasive cervical cancer [ [29] , [30] , [31] ].…”

Section: Study Rationalementioning

confidence: 99%

A novel cervical cancer screen-triage-treat demonstration project with HPV self-testing and thermal ablation for women in Malawi: Protocol for a single-arm prospective trial

Chinula

McGue

Smith

et al. 2022

Contemporary Clinical Trials Communications

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Section: Study Rationalementioning

confidence: 99%

A novel cervical cancer screen-triage-treat demonstration project with HPV self-testing and thermal ablation for women in Malawi: Protocol for a single-arm prospective trial

Chinula

McGue

Smith

et al. 2022

Contemporary Clinical Trials Communications

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“…HPV detection in urine samples may be due to the contamination of HPV-infected cells from the external urethral orifice or distal urethra, T A B L E 2 HPV prevalence and type distribution in urine and bladder washing samples (n = 201) which are the most common anatomic sites of HPV infection among the urinary tract in females and males. 1,6,8,9 Therefore, urine samples were unlikely to be suitable for HPV infection detection in the urinary bladder.…”

Section: Discussionmentioning

confidence: 99%

“…Urine is a most convenient and simple tool for screening HPV infection in the urinary tract; however, identifying the anatomic sites of HPV infection in the urinary tract in cases with HPV‐deoxyribonucleic acid (DNA) detection in spontaneously voided urine is difficult. Indeed, epithelial cells exfoliated from the external genitalia, external orifice, and distal urethra are often contaminated in urine samples in females 8,9 . Contrastingly, HPV detection is the lowest in samples of the urogenital in males, such as the urethra, urine, and semen, and especially urine sample is reported to be inadequate for HPV detection 1,6 …”

Section: Introductionmentioning

confidence: 99%

“…Indeed, epithelial cells exfoliated from the external genitalia, external orifice, and distal urethra are often contaminated in urine samples in females. 8,9 Contrastingly, HPV detection is the lowest in samples of the urogenital in males, such as the urethra, urine, and semen, and especially urine sample is reported to be inadequate for HPV detection. 1,6 However, we have recently reported HPV prevalence in urine samples by applying liquid-based cytology and demonstrated that the urinary tract can be an alternative frequent site of HPV infection in males.…”

mentioning

confidence: 99%

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Are bladder washing samples suitable for investigation of HPV infection in urinary bladder? Comparison in HPV prevalence between urine and washing samples

Nakagawa

Shigehara

Kato

et al. 2022

Journal of Medical Virology

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Although urine and bladder washing samples are commonly used for the cytological evaluation of the bladder mucosa, it has been unknown whether these samples are likely suitable to investigate human papillomavirus (HPV) prevalence in the urinary bladder. The present study aimed to elucidate the appropriateness of spontaneously voided urine or bladder washing in screening HPV infection in the urinary bladder. Urine and bladder washing samples were obtained from 201 patients who underwent transurethral bladder tumor resection. After extracting DNA from both samples, HPV‐DNA was examined using a nested polymerase chain reaction with GP5+/6+ and MY09/11 primers. HPV genotyping was performed in the HPV‐positive samples. In situ hybridization (ISH) was performed to observe the HPV‐DNA localization in urothelial cells among cytological samples and paraffin‐embedded tumor tissues in HPV‐positive washing samples. HPV prevalence in urine and washing samples were 9.5% and 7.0%, respectively. High‐risk HPV prevalence in urine and washing samples was 7.5% and 4.0%, respectively. The most common HPV type was HPV 16, followed by HPV 52 and HPV 18 in both samples. HPV type distribution in both samples was not in agreement (κ = −0.431). The ISH analysis revealed that HPV‐DNA signal was observed in urothelial cells of five (55.7%) of nine detectable HPV‐positive cytological samples. Six (66.7%) of nine HPV‐positive cases had HPV‐DNA signals in tumor tissue. The use of washing samples was likely applicable for investigating HPV prevalence in the urinary bladder. HPV‐DNA detected in washing samples might be frequently derived from the urinary bladder.

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“…20 In the study by Maged et al, to assess the sensitivity of urine test for detection of hrHPV on 1375 women referred to a medical school in Cairo, 87 women had positive urine test for high-risk HPV and 82 had pathologic findings of CIN 2 or CIN 3. 23 In a cross sectional study by Sargent et al, to compare the sensitivity of hrHPV testing for detection of CIN2+ in matched urine and cervical samples, the sensitivity of urine for detection of CIN2+ lesions was 83% for urine and 89% for cervical sample. 24 Ornskov et al conducted a study between paired urine, cervical and vaginal specimen.…”

Section: Sensitivity and Specificity Of Urine Test To Detect Cin 2+mentioning

confidence: 99%

Concordance between urine and self-collected vaginal samples for high-risk HPV detection in cervical cancer screening

et al. 2023

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Background: A system comprising of screening for pre-cancerous lesions succeeded by therapeutic interventions can greatly reduce the incidence of cervical cancer. High risk HPV detection in urine has proven itself as a potential primary screening tool. However, only a few studies have showed it’s concordance with matched self-collected vaginal samples while screening for high risk HPV infection in the community. Objective of the study was to determine the concordance between first void urine sample and self-collected vaginal sample for high risk HPV detection using Cobas 4800. The sensitivity and positive predictive value of screening by urine HPV testing for CIN 2+ detection would also be done along with its acceptability and cost analysis in community screening. Methods: This study will include women between 30-60 years with intact cervix and no precancerous lesions. Self-sampling will be provided by participants in the form of urine and vaginal swab collection by the Collipee device and vaginal swab stick respectively. This will be followed by high risk HPV testing using Cobas 4800 technology. The participants will also be asked to fill up a questionnaire about the acceptability of urine sampling for cancer screening. Conclusions: Screening tools can perhaps be diversified in order to suit the needs of low and middle income countries. Research and development of cervical cancer screening tools could hence bring to light feasible alternatives that could further improve compliance of non-attenders. Trial registration: The trial is registered with clinical trials registry (CTRI/2023/01/049322).

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Urine test for HPV genotypes as a predictor of precancerous cervical lesions and for cervical cancer screening

Abstract: There was good agreement between a positive urine test for high-risk HPV DNA genotypes and pathologic findings of CIN2/3.

Cited by 9 publications

References 21 publications

A novel cervical cancer screen-triage-treat demonstration project with HPV self-testing and thermal ablation for women in Malawi: Protocol for a single-arm prospective trial

A novel cervical cancer screen-triage-treat demonstration project with HPV self-testing and thermal ablation for women in Malawi: Protocol for a single-arm prospective trial

Are bladder washing samples suitable for investigation of HPV infection in urinary bladder? Comparison in HPV prevalence between urine and washing samples

Concordance between urine and self-collected vaginal samples for high-risk HPV detection in cervical cancer screening

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