Lineage variability in surface components expression within Porphyromonas gingivalis

Teixeira, Sílvia Regina Loureiro; D'Epiro, Talyta Thereza Soares; Pinheiro, Éricka Tavares; Simionato, Maria Regina Lorenzetti; Taniwaki, Noemi Nosomi; Kisielius, Jonas José; Mayer, Márcia Pinto Alves

doi:10.1016/j.micpath.2014.11.001

Cited by 4 publications

(7 citation statements)

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Section: Discussionmentioning

confidence: 99%

“…Nevertheless, P. gingivalis presents a high diversity as indicated by their different virulent surface components [32]. P. gingivalis W83 is a capsulated/afimbriated highly virulent strain, whereas ATCC 33277 is a no capsulated/fimbriated less virulent strain [32,33]. These P. gingivalis strains also differ in relation to biofilm formation [34] and chromosomal transposable elements, which may alter their gene transcription [35], and physiology, such as their capacity to survive under oxidative stress [36], which altogether can contribute to explain differences on biofilm composition and transcription of virulence factors induced by probiotics.…”

Section: Discussionmentioning

confidence: 99%

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Probiotics alter biofilm formation and the transcription of Porphyromonas gingivalis virulence-associated genes

Ishikawa

Mita

Kawamoto

et al. 2020

Journal of Oral Microbiology

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Background and Objective: The potential of probiotics on the prevention and control of periodontitis and other chronic inflammatory conditions has been suggested. Lactobacillus and Bifidobacterium species influence P. gingivalis interaction with gingival epithelial cells (GECs) but may not act in a unique way. In order to select the most appropriate probiotic against P. gingivalis, we aimed to evaluate the effect of several strains on Porphyromonas gingivalis biofilm formation and transcription virulence-associated factors (PgVAFs). Methods: Cell-free pH neutralized supernatants (CFS) and living Lactobacillus spp. and Bifidobacterium spp. were tested against P. gingivalis ATCC 33277 and W83, in mono-and multi-species (with Streptococcus oralis and S. gordonii) biofilms. Relative transcription of P. gingivalis genes (fimA, mfa1, kgp, rgp, ftsH and luxS) was determined in biofilms and under GECs co-infection. Results: Probiotics CFS reduced P. gingivalis ATCC 33277 levels in mono-species biofilms and living probiotics reduced P. gingivalis abundance in multi-species biofilms. L. acidophilus LA5 down-regulated transcription of most PgVAFs in biofilms and GECs. Conclusions: Probiotics affect P. gingivalis biofilm formation by down-regulating overall PgVAFs with the most pronounced effect observed for L. acidophilus LA5.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Probiotics alter biofilm formation and the transcription of Porphyromonas gingivalis virulence-associated genes

Ishikawa

Mita

Kawamoto

et al. 2020

Journal of Oral Microbiology

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“…Cell density and iron/heme availability are important signals affecting the physiology of bacterial cells and are important for the pathogenicity of P. gingivalis . However, it has been suggested that some strains may have the capacity to cause dysbiosis and disease, and differences between strains in the ability to cause localized or systemic infections have been observed in animal model studies [41–47]. For example, the ATCC 33277 strain (non-encapsulated) forms higher biofilm structures, is highly fimbriated, but less virulent, whereas the A7436 strain (encapsulated) is less fimbriated, produces smaller biofilm structures, but exhibits higher virulence in animal model studies [48–52].…”

Section: Discussionmentioning

confidence: 99%

“…For example, the ATCC 33277 strain (non-encapsulated) forms higher biofilm structures, is highly fimbriated, but less virulent, whereas the A7436 strain (encapsulated) is less fimbriated, produces smaller biofilm structures, but exhibits higher virulence in animal model studies [48–52]. Several reports have shown that diversity in production of specific virulence factors by P. gingivalis might be caused by several mechanisms, including specific domain rearrangements and allelic exchange [43, 47, 51, 53–56]. One example is the two-component regulatory system composed of the histidine kinase HaeS and the response regulator HaeR.…”

Section: Discussionmentioning

confidence: 99%

PgFur participates differentially in expression of virulence factors in more virulent A7436 and less virulent ATCC 33277 Porphyromonas gingivalis strains

et al. 2019

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Background Porphyromonas gingivalis is considered a keystone pathogen responsible for chronic periodontitis. Although several virulence factors produced by this bacterium are quite well characterized, very little is known about regulatory mechanisms that allow different strains of P. gingivalis to efficiently survive in the hostile environment of the oral cavity, a typical habitat characterized by low iron and heme concentrations. The aim of this study was to characterize P. gingivalis Fur homolog (PgFur) in terms of its role in production of virulence factors in more (A7436) and less (ATCC 33277) virulent strains. Results Expression of a pgfur depends on the growth phase and iron/heme concentration. To better understand the role played by the PgFur protein in P. gingivalis virulence under low- and high-iron/heme conditions, a pgfur -deficient ATCC 33277 strain (TO16) was constructed and its phenotype compared with that of a pgfur A7436-derived mutant strain (TO6). In contrast to the TO6 strain, the TO16 strain did not differ in the growth rate and hemolytic activity compared with the ATCC 33277 strain. However, both mutant strains were more sensitive to oxidative stress and they demonstrated changes in the production of lysine- (Kgp) and arginine-specific (Rgp) gingipains. In contrast to the wild-type strains, TO6 and TO16 mutant strains produced larger amounts of HmuY protein under high iron/heme conditions. We also demonstrated differences in production of glycoconjugates between the A7436 and ATCC 33277 strains and we found evidence that PgFur protein might regulate glycosylation process. Moreover, we revealed that PgFur protein plays a role in interactions with other periodontopathogens and is important for P. gingivalis infection of THP-1-derived macrophages and survival inside the cells. Deletion of the pgfur gene influences expression of many transcription factors, including two not yet characterized transcription factors from the Crp/Fnr family. We also observed lower expression of the CRISPR/Cas genes. Conclusions We show here for the first time that inactivation of the pgfur gene exerts a different influence on the phenotype of the A7436 and ATCC 33277 strains. Our findings further support the hypothesis that PgFur regulates expression of genes encoding surface virulence factors and/or genes involved in their maturation. Electronic supplementary material The online version of this article (10.1186/s12866-019-1511-x) contains supplementary material, which is available to authorized users.

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“…A participação de P. gingivalis no biofilme dental é mediada especialmente pela fímbria FimA, envolvida na formação de biofilme monoespécie e na co-agregação aos colonizadores iniciais do biofilme dental (17,18) como Streptococcus gordonii e Streptococcus oralis (9). No entanto, muitas cepas de P. gingivalis não apresentam a fímbria FimA1 (19), incluindo cepas de virulência reconhecida, como a cepa W83 (20) e a cápsula é a estrutura que medeia a coagregação de P. gingivalis nesta amostra (21). A cápsula de polissacarídeo é também implicada na evasão das defesas do hospedeiro.…”

Section: Microbiota Associada à Periodontiteunclassified

Efeito de probióticos do gênero Lactobacillus sobre a perda óssea alveolar promovida por periodontite induzida em camundongos

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Lineage variability in surface components expression within Porphyromonas gingivalis

Cited by 4 publications

References 48 publications

Probiotics alter biofilm formation and the transcription of Porphyromonas gingivalis virulence-associated genes

Probiotics alter biofilm formation and the transcription of Porphyromonas gingivalis virulence-associated genes

PgFur participates differentially in expression of virulence factors in more virulent A7436 and less virulent ATCC 33277 Porphyromonas gingivalis strains

Efeito de probióticos do gênero Lactobacillus sobre a perda óssea alveolar promovida por periodontite induzida em camundongos

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