Effects and action mechanisms of sodium fluoride (NaF) on the growth and cephalotaxine production of Cephalotaxus mannii suspension cells

Li, Yongcheng; Jiang, Xuan-Xian; Long, Xiao-Juan

doi:10.1016/j.enzmictec.2014.09.006

Cited by 11 publications

(2 citation statements)

References 27 publications

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“…The exogenous application of ASA most likely significantly increases the activity of the antioxidant system, directly impacting the intensity of photosynthesis. Li and his co-workers ( 2014 ) reported that SA significantly enhances Torreya grandis biomass under salinity stress due to the improvement in chlorophyll content and the flow of antioxidant enzyme activity that consequently alleviates oxidative stress and enhances the photosynthetic process. Furthermore, the exogenous application of SA effectively prevents iron deficiency, which is the primary cause of plant chlorosis (Kong et al 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Acetylsalicylic acid enhance tolerance of Phaseolus vulgaris L. to chilling stress, improving photosynthesis, antioxidants and expression of cold stress responsive genes

et al. 2018

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BackgroundHigh and low temperatures constitute the most damaging type of abiotic stress and limit the survival, and productivity of plants. The present study aimed to evaluate the role of exogenous applications of acetylsalicylic acid (ASA) in reducing the deleterious effects of cold stress. Phaseolus vulgaris L. seedlings were treated with foliar-sprayed ASA at concentrations of 0–3 mM and then subjected to chilling stress at 4 °C for 2 or 4 days.ResultsGrowth, photosynthesis, biochemical alterations, oxidative damage and antioxidant enzyme activities as well as the expression of cold-responsive genes (CBF3–COR47), were monitored during the experiment. ASA applications substantially improved several growth and photosynthetic parameters, including shoot biomass, dry weight, and photosynthetic pigments, of P. vulgaris seedlings exposed to different durations of chilling stresses. The ASA foliar spray treatments significantly (p < 0.05) rescued the growth and photosynthetic pigments of P. vulgaris seedlings under different chilling stresses. The total soluble sugars markedly increased during 0–4 days of chilling stress following ASA foliar spraying. The exogenous application of ASA significantly (p < 0.05) increased the accumulation of proline in P. vulgaris seedlings under chilling stress. At the gene expression level, ASA significantly (p < 0.05) upregulated the cold-responsive genes CBF3 and COR47.ConclusionsAs a result, we speculate that, the application of exogenous ASA alleviated the adverse effects of chilling stress on all measured parameters, and 1 and 2 mM ASA exhibited the greatest effects.

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Section: Discussionmentioning

confidence: 99%

Acetylsalicylic acid enhance tolerance of Phaseolus vulgaris L. to chilling stress, improving photosynthesis, antioxidants and expression of cold stress responsive genes

et al. 2018

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“…However, the yields of this highly promising compound from natural sources are very low. This has motivated the realization of various studies aimed to enhance the production of HHT [11] and cephalotaxine [12,13]. Therefore, accurate, sensitive, and rapid analytical methods are needed to screen for superior breeding lines producing high amounts of Cephalotaxus alkaloids.…”

Section: Introductionmentioning

confidence: 99%

A Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay for Determination of Homoharringtonine

et al. 2018

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Homoharringtonine (HHT), also known as omacetaxine, is a natural compound found in the genus and is a promising pharmaceutical drug used for the treatment of chronic or accelerated phase chronic myeloid leukemia. As a tool for the quantitative determination of HHT, a specific monoclonal antibody against HHT (MAb 6A1) was generated by conjugates prepared via sodium periodate-mediated oxidation. The developed indirect competitive enzyme-linked immunosorbent assay (icELISA) using MAb 6A1 was found to be highly specific and sensitive with a limit of detection for HHT of 48.8 ng/mL. Validation assays to evaluate precision and accuracy of the method were conducted by the use of intra- and inter-assay analysis, recovery test, and comparison analysis between the amounts of HHT determined by ELISA and high-performance liquid chromatography. These results revealed that the established icELISA using MAb 6A1 is specific, sensitive, and reliable enough to be applied to the qualitative analysis for HHT. Furthermore, the results of this study support the usefulness of sodium periodate as a reagent for the conjugation between alkaloids and proteins for producing specific antibodies.

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The Alkaloidal Constituents of Cephalotaxus mannii Collected in Lam Dong Province, Vietnam

Loc¹,

Liễu

Thảo³

et al. 2017

Chem Nat Compd

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Effects and action mechanisms of sodium fluoride (NaF) on the growth and cephalotaxine production of Cephalotaxus mannii suspension cells

Cited by 11 publications

References 27 publications

Acetylsalicylic acid enhance tolerance of Phaseolus vulgaris L. to chilling stress, improving photosynthesis, antioxidants and expression of cold stress responsive genes

Acetylsalicylic acid enhance tolerance of Phaseolus vulgaris L. to chilling stress, improving photosynthesis, antioxidants and expression of cold stress responsive genes

A Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay for Determination of Homoharringtonine

The Alkaloidal Constituents of Cephalotaxus mannii Collected in Lam Dong Province, Vietnam

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