Metabolite secretion, Fe3+-reducing activity and wood degradation by the white-rot fungus Trametes versicolor ATCC 20869

Aguiar, André; Gavioli, Daniela; Ferraz, André

doi:10.1016/j.funbio.2014.08.004

Cited by 16 publications

(8 citation statements)

References 44 publications

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“…T. versicolor is primarily known as a wood‐decay and decomposer fungus (Aguiar et al. ). Several studies have demonstrated that Trametes spp., including T. versicolor , can grow endophytically in the sapwood, needles, fruits, and twigs of woody plants (Martin et al.…”

Section: Discussionmentioning

confidence: 99%

Invisible but consequential: root endophytic fungi have variable effects on belowground plant–insect interactions

2017

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Abstract. Endophytic fungi are ubiquitous in nature and can play important roles in regulating plantherbivore interactions. While some aboveground obligate symbionts are considered defensive mutualists of host plants, the importance of root endophytes in plant defense, especially against root-feeding insects, remains unclear. This study aimed to investigate the effects of root fungal endophytes on plant resistance against belowground herbivores and the recovery of host plants from damage. We grew the common grass Festuca arundinacea (tall fescue) semi-aeroponically in the laboratory and inoculated roots with one of five fungal endophytes isolated from field-collected tall fescue or meadow soil. Endophyte-inoculated and uninoculated control plants were subjected to feeding by larvae of the generalist root herbivore Rhizotrogus majalis (European chafer). Herbivory intensity was quantified after eight days, and regrowth of roots and shoots following root herbivory and mechanical shoot damage was measured thereafter for each treatment. Fungal identifications by DNA sequence analysis were conducted after completion of the herbivory experiments and revealed that the five endophytes included the decomposer fungi Trametes versicolor and Mortierella alpina, the entomopathogenic fungi Isaria fumosorosea and Beauveria bassiana, and a potential plant pathogen/entomopathogen Fusarium cf. equiseti. The effects of these root endophytes on plant defense against root-feeding insects were species-specific. While four endophytes had few effects on plant resistance, endophytic B. bassiana significantly reduced herbivore damage to roots. In comparison, plant tolerance to damage was impaired after colonization by all endophyte species except T. versicolor and M. alpina. The contrasting effects of endophytes on plant resistance and plant tolerance suggest that research solely evaluating plant resistance is likely to overestimate the benefits conferred by endophytes without accounting for potential negative effects on plant tolerance. We propose a conceptual framework to include both plant resistance and tolerance as two dimensions of a defensive strategy and show that plant associations with different root endophytes may shift the relative importance of resistance and tolerance for plant defense.

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Section: Discussionmentioning

confidence: 99%

Invisible but consequential: root endophytic fungi have variable effects on belowground plant–insect interactions

2017

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“…6,12 Organosolv pretreatment results in higher yields of pure cellulose with reduced crystallinity and degree of polymerization that are desirable outcomes in pretreatment choices as applied to conversion of biomass in bio-renery operations. 8,9 Our results demonstrated that the organosolv pretreatment resulted in 100% saccharication within 24 h of enzymatic hydrolysis, with or without fungal pretreatment.…”

Section: Discussionmentioning

confidence: 99%

“…3,4 White-rot and brown-rot fungi are commonly used for biodegradation of lignocellulosic biomass; they act by depolymerizing and/or mineralizing lignin and in some cases, hemicellulose. Previously published studies explore the mechanistic actions of white-rot fungi such as Ceriporiopsis subvermispora and Trametes versicolor in reducing the recalcitrance of lignocellulosic biomass and consequently, improving its enzymatic digestibility; 5,6 however, only a few studies present its potential in terms of lignin isolation. 7 Biological pretreatment is oen combined with chemical pretreatments, for example, the organosolv process, which uses organic solvents such as, ethanol, methanol, butanol and acetone, at high temperatures ranging between 150 and 200 C, to fractionate lignocellulosic biomass.…”

Section: Introductionmentioning

confidence: 99%

Beneficial effects of Trametes versicolor pretreatment on saccharification and lignin enrichment of organosolv-pretreated pinewood

et al. 2017

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While previous studies have shown that white-rot fungal pretreatment reduces the severity of chemical pretreatments and improves enzymatic saccharification yields, very few have investigated the synergistic effects of fungal pretreatment combined with organosolv pretreatment on lignin recovery and quality. In this study, loblolly pine chips were incubated with Trametes versicolor for 15, 30 and 45 days, prior to organosolv pretreatment and enzymatic saccharification. Fungal pretreatment for 15 days improved the saccharification yield by 23%, and higher amounts (56%) of lignin-enriched fractions were obtained.Fungal pretreatment for 45 days led to extensive depolymerization, structural modification and enrichment of lignin in the organosolv precipitates (OP). Characterization of the OP fraction showed that samples pretreated for 30 and 45 days contained higher amounts of hydroxyl groups and phydroxyphenyl (H) subunits, and showed increases in depolymerization of carbohydrates as well as decreases in G/H lignin ratio. It became apparent that further investigations are needed to explore the benefits of combining fungal and organosolv pretreatments for improving lignin yields from softwoods.

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“…The best lignin degrading strain was selected out of a total of eight strains reported for pretreatment in literature (Aguiar et al 2014;Chang et al 2012;Knezevic et al 2013;Taniguchi et al 2005;Zhang et al 2007) based on the highest ligninolytic and low cellulolytic abilities (Online Resource 1) and maximum SV (lignin degradation/cellulose loss) during pretreatment of SSB in SSF (Online Resource 2).…”

Section: Screening Of Selective Strainsmentioning

confidence: 99%

“…Fungal strain having high selectivity was screened initially out of different strains reported to be used in the pretreatment (Aguiar et al 2014;Chang et al 2012;Knezevic et al 2013;Taniguchi et al 2005;Zhang et al 2007). The detailed studies on the alteration of the enzyme profiles have explained the effect on lignin degradation, SV and sugar yield after enzymatic hydrolysis.…”

Section: Introductionmentioning

confidence: 99%

Fungal pretreatment of sweet sorghum bagasse with supplements: improvement in lignin degradation, selectivity and enzymatic saccharification

Mishra

Jana

et al. 2017

3 Biotech

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Sweet sorghum bagasse (SSB) from food processing and agricultural industry has attracted the attention for uses in production of biofuel, enzymes and other products. The alteration in lignocellulolytic enzymes by use of supplements in fungal pretreatment of SSB to achieve higher lignin degradation, selectivity value and enzymatic hydrolysis to fermentable sugar was studied. Fungal strain Coriolus versicolor was selected for pretreatment due to high ligninolytic and low cellulolytic enzyme production resulting in high lignin degradation and selectivity value. SSB was pretreated with supplements of veratryl alcohol, syringic acid, catechol, gallic acid, vanillin, guaiacol, CuSO 4 and MnSO 4 . The best results were obtained with CuSO 4 , gallic acid and syringic acid supplements. CuSO 4 increased the activities of laccase (4.9-fold) and polyphenol oxidase (1.9-fold); gallic acid increased laccase (3.5-fold) and manganese peroxidase (2.5-fold); and syringic acid increased laccase (5.6-fold), lignin peroxidase (13-fold) and arylalcohol oxidase (2.8-fold) resulting in enhanced lignin degradations and selectivity values than the control. Reduced cellulolytic enzyme activities resulted in high cellulose recovery. Enzymatic hydrolysis of pretreated SSB yielded higher sugar due to degradation of lignin and reduced the crystallinity of cellulose. The study showed that supplements could be used to improve the pretreatment process. The results were confirmed by scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy and thermogravimetric/differential thermogravimetric analysis of SSB.

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Metabolite secretion, Fe3+-reducing activity and wood degradation by the white-rot fungus Trametes versicolor ATCC 20869

Cited by 16 publications

References 44 publications

Invisible but consequential: root endophytic fungi have variable effects on belowground plant–insect interactions

Invisible but consequential: root endophytic fungi have variable effects on belowground plant–insect interactions

Beneficial effects of Trametes versicolor pretreatment on saccharification and lignin enrichment of organosolv-pretreated pinewood

Fungal pretreatment of sweet sorghum bagasse with supplements: improvement in lignin degradation, selectivity and enzymatic saccharification

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