Expression of key executioner of apoptosis caspase‐3 in periodontal health and disease

Pradeep, A.R.; Suke, Deepak Kumar; Prasad, M. V. R.; Singh, Sonender Pal; Martande, Santosh S.; Nagpal, Kanika; Naik, Savitha B.; Guruprasad, C. N.; Raju, Arjun; Singh, Priyanka; Siddaya, Math

doi:10.1111/jicd.12134

Cited by 34 publications

(28 citation statements)

References 44 publications

(64 reference statements)

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“…However, the current study did find that mitochondrial protein AIF levels were significantly higher in the CP group compared to the group Healthy ( P < 0.01). Because AIF is necessary for T‐cell function, and periodontal pathogens induce apoptosis in T cells; according to current results intrinsic pathway involved caspase‐independent AIF may play a role in CP.…”

Section: Discussionmentioning

confidence: 55%

The role of caspase‐8, caspase‐9, and apoptosis inducing factor in periodontal disease

Aral

Kapila

2018

Journal of Periodontology

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Background Caspases are key mediators of apoptosis. Caspase‐8 mediates extrinsic, and caspase‐9 initiates the intrinsic pathway of apoptosis. Apoptosis Inducing Factor (AIF), a mitochondrial proapoptotic protein, mediates cell death by a caspase‐independent process. Because apoptosis is involved in periodontal disease, this study evaluated caspase‐8, ‐9, and AIF in periodontal disease. Methods Twenty periodontally healthy volunteers (Group Healthy), 20 patients with generalized aggressive periodontitis (Group AgP), and 20 patients with generalized chronic periodontitis (Group CP) were included in this study. Levels of caspase‐8, ‐9, and AIF were evaluated in gingival crevicular fluid (GCF) of all participants via enzyme‐linked immunosorbent assays. Results AIF was significantly higher in the AgP (P = 0.07) and CP groups (P = 0.01) than the Healthy group, and similar to the CP and AgP groups (P > 0.05). Caspase‐8 was significantly higher in the CP and Healthy groups than the AgP group (P = 0.00), and similar between Healthy and CP groups (P > 0.05). Caspase‐9 was significantly higher in the AgP group than the Healthy group (P = 0.01), and similar between Healthy and CP groups (P > 0.05). Conclusions The mitochondrial‐centered intrinsic pathway involving caspase‐9 and AIF, and the extrinsic pathway involving caspase‐8 are significant for aggressive periodontitis. The intrinsic pathway involving caspase‐independent AIF is also significant for chronic periodontitis.

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Section: Discussionmentioning

confidence: 55%

The role of caspase‐8, caspase‐9, and apoptosis inducing factor in periodontal disease

Aral

Kapila

2018

Journal of Periodontology

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“…There were no significant differences between groups regarding caspase‐3 expression. Pradeep et al assessed the expression of caspase‐3 in serum and gingival crevicular fluid samples and found significantly increased values in patients with periodontitis . They found a significant gradual increase of caspase‐3 levels between the three groups (healthy, gingivitis, and periodontitis).…”

Section: Discussionmentioning

confidence: 99%

“…However, studies have also reported significantly higher levels of pro‐apoptotic markers in gingival crevicular fluid associated with patients with periodontitis when compared to controls . Likewise, Listyarifah et al found evidence of a significantly higher number of apoptotic cells in gingival biopsy samples of patients with chronic periodontitis compared to patients with gingivitis .…”

Section: Introductionmentioning

confidence: 99%

Anti‐apoptotic traits in gingival tissue from patients with severe generalized chronic periodontitis

Figueredo

Alves

Beiler

et al. 2019

J of Invest & Clin Dent

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Aim In the present study, we aimed to evaluate the cytosolic and nuclear‐mitochondrial expression of pro‐apoptotic and anti‐apoptotic markers in gingival tissue from patients with severe generalized chronic periodontitis (sGCP). Methods Twenty‐four patients participated in the study: 15 (54.3 + 8.3 years) with sGCP and nine (38.2 + 5.4 years) with gingivitis alone. Gingival tissue was collected using a 1.5‐mm diameter punch and homogenized using a cell disruptor. The supernatants were analyzed for the cytosolic and nuclear‐mitochondrial fractions of caspase‐3, Bax, Bak, Smac, lamin B, Bad, Bim, survivin, Bcl‐xL, Mcl‐1, and of the dimers Bcl‐2/Bax, Bcl‐xL/Bak and Mcl‐1/Bak using a multiplex immunoassay. Results Significantly higher levels of cytosolic Bcl‐xL/Bak, nuclear‐mitochondrial Mcl‐1/Bak, and cytosolic Bcl‐xL were observed in gingival tissues from periodontitis patients compared to controls (P = 0.03, 0.03, and 0.05, respectively). The patients with gingivitis presented significantly increased levels of nuclear‐mitochondrial Bad, cytosolic and nuclear‐mitochondrial Bcl‐2/Bax, and cytosolic Bim compared to the patients with periodontitis (P < 0.001, 0.03, 0.05, and 0.04, respectively). Conclusion Significantly higher levels of anti‐apoptotic markers, such as Bcl‐xL/Bak, Mcl‐1/Bak, and Bcl‐xL, and lower levels of pro‐apoptotic markers Bad and Bim in inflamed tissues indicate an anti‐apoptotic trait in patients with sGCP.

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“…A number of previous enquiries have established an association between caspases and chronic periodontitis but none have reported a link between this condition and CASP gene polymorphism. For example, the concentration of caspase 3 in gingival crevicular fluid and serum has been seen to proportionally increase with the progression of periodontal disease (Abuhussein et al, 2014;Pradeep et al, 2014). Moreover, Aggregatibacter actinomycetemcomitans infection enhances apoptosis of osteoblastic cells, reducing osseous coupling through a caspase 3-dependent mechanism (Kang et al, 2012;Pacios et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…For instance, Aggregatibacter actinomycetemcomitans infection enhances apoptosis through a caspase 3-dependent mechanism (Kang et al, 2012). Furthermore, caspase 3 expression increases with progression of gingival inflammation, probing depth, and clinical attachment level in periodontitis (Pradeep et al, 2014), and tissue destruction by Porphyromonas gingivalis involves both caspase 1-dependent and -independent mechanisms (Taxman et al, 2006). Activation of caspases 3 and 7 is considerably enhanced in gingival tissue from periodontitis patients (Bantel et al, 2005), in whose osteoblasts the activation of caspase 8 has also been observed (Mori et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Assessment of CASP gene polymorphisms in periodontal disease

Sw¹,

Kim²,

Chung³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Caspases (CASP) are intracellular proteases that play roles as mediators of apoptosis. Activation of caspase 3 is enhanced in chronic periodontitis. Thus, we hypothesized that single nucleotide polymorphisms (SNPs) of CASP genes might be associated with this condition in the Korean population. To investigate whether such polymorphisms might be involved in the development of periodontal disease, 51 patients and 33 control subjects were assessed. A total of 201 CASP gene SNPs were analyzed with genotypes being determined using and Axiom™ genomewide human assay. SNPStats and SPSS 18.0 were used for the analysis of genetic data and logistic regression models were utilized to evaluate odds ratios, 95% confidence intervals, and P values. Of the 201 SNPs, only three (rs12108497, rs4647602, and rs113420705, all in the CASP3 gene) were significantly associated with chronic periodontitis (P < 0.05). The minor allele frequencies of these SNPs were higher in the patient group than in the control group. In addition, the TC and GT haplotypes 18070 S.W. Kang et al. ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 18069-18077 (2015) formed by rs4647602 and rs113420705 were found to be associated with chronic this disease (TC haplotype, P = 0.0039; GT haplotype, P = 0.002). These results suggest that CASP3 gene polymorphisms may be associated with susceptibility to periodontal disease in the Korean population.

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Expression of key executioner of apoptosis caspase‐3 in periodontal health and disease

Abstract: GCF and the serum concentration of caspase-3 proportionally increases with the progression of periodontal disease, that is, gingival inflammation, PD, and CAL.

Cited by 34 publications

References 44 publications

The role of caspase‐8, caspase‐9, and apoptosis inducing factor in periodontal disease

The role of caspase‐8, caspase‐9, and apoptosis inducing factor in periodontal disease

Anti‐apoptotic traits in gingival tissue from patients with severe generalized chronic periodontitis

Assessment of CASP gene polymorphisms in periodontal disease

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