Differential expression profiling of microRNAs in para-carcinoma, carcinoma and relapse human pancreatic cancer

Lai, Xiaolong; Huang, Yu; Li, Y.-S.; Li, G.-N.; Wang, L.-P.; Sun, Ryan; Ma, Yu‐Shui; Feng, Shengyu; Chang, Zhengyan; Wang, X.-H.; Fu, Da; Han, Xu; Cong, Xianling; Li, W.-P.

doi:10.1007/s12094-014-1249-8

Cited by 8 publications

(6 citation statements)

References 26 publications

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“…In almost all malignancy studies, microRNA (miRNAs) are found especially critical because they can be the results of chromosome lesions, modulated by classic cell signaling, and they themselves can act as both oncogenes and tumor suppressors ( Bartel, 2004 ; Lan et al, 2015 ). Recently, a close association between miRNAs and pancreatic cancer tumorigenesis has been elucidated ( Lai et al, 2015 ; Pavlakis et al, 2013 ). Various miRNAs, such as miR-200 ( Yu et al, 2010 ), miR-146a ( Li et al, 2010 ), miR-486 ( Mees et al, 2009 ) and the let-7 family ( Li et al, 2009b ) have been confirmed to be tumor suppressors; meanwhile, pancreatic cancer malignancy was found positively associated with miR-196a ( Huang et al, 2014 ), miR-212 ( Ma et al, 2014 ) and miR-31 ( Laurila et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

MiR-142 modulates human pancreatic cancer proliferation and invasion by targeting hypoxia-inducible factor 1 (HIF-1α) in the tumor microenvironments

Wei

et al. 2017

Biology Open

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MicroRNAs regulate most protein-coding genes, including genes important in cancer and other diseases. In this study, we demonstrated that the expression of miR-142 could be significantly suppressed in pancreatic cancer specimens and cell lines compared to their adjacent tissues and normal pancreatic cells. Growth and invasion of PANC-1 and SW1990 cells were attenuated by overexpression of miR-142 in vitro. With the help of bioinformatics analysis, hypoxia-inducible factor 1 (HIF-1α) was identified to be a direct target of miR-142, and a luciferase reporter experiment confirmed this discovery. Overexpression of miR-142 decreases protein expression of HIF-1α. In the hypoxic microenvironment, HIF-1α was up-regulated while miR-142 was down-regulated. The invaded cells significantly increased in the hypoxic microenvironment compared to the normoxic microenvironment. The hypoxia treatment induced cells’ proliferation, and invasion could be inhibited by miR-142 overexpression or HIF-1α inhibition. Moreover, expression of epithelial-mesenchymal transition (EMT) markers, Vimentin, VEGF-C and E-cad, was altered under hypoxia conditions and regulated by miR-142/HIF-1α. Above all, these findings provided insights on the functional mechanism of miR-142, suggesting that the miR-142/HIF-1α axis may interfere with the proliferative and invasive properties of pancreatic cancer cells, and indicated that miR-142 could be a potential therapeutic target for pancreatic cancer.

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Section: Introductionmentioning

confidence: 99%

MiR-142 modulates human pancreatic cancer proliferation and invasion by targeting hypoxia-inducible factor 1 (HIF-1α) in the tumor microenvironments

Wei

et al. 2017

Biology Open

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“…MicroRNAs (miRNAs) are particularly important in almost all tumor developmental processes since they can be targets of genomic lesions, controlled by classic tumor signals, and they themselves present as a class of oncogenes or tumor suppressors (3,4). Recently, a close association between miRNAs and lung cancer tumorigenesis has been suggested (5,6). Multiple miRNAs, such as the let-7 family (7), miR-200 (8), miR-486 (9) and miR-146a (10) have been identified as tumor suppressors.…”

Section: Introductionmentioning

confidence: 99%

lncRNA XIST interacts with miR-140 to modulate lung cancer growth by targeting iASPP

Tang

et al. 2017

Oncology Reports

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X-inactive specific transcript (XIST), one of the first found cancer-associated long non-coding RNAs (lncRNAs), is involved in the development and progression of many types of tumors. Aberrant expression of XIST has been observed in hepatocellular carcinoma, cervical, breast, ovarian and colorectal cancer. However, the exact effects and molecular mechanisms of XIST in lung cancer progression are still unknown to date. In the present study, we investigated the role of XIST in human lung cancer cell lines and clinical tumor samples in order to determine the function of this molecule. In our research, lncRNA-XIST was specifically upregulated in lung cancer cell lines and promoted lung cancer cell growth through targeting miR-140. Knockdown of XIST inhibited the proliferation and promoted cell apoptosis of human lung cancer cells and suppressed metastasis in vitro and in vivo. In addition, miR-140-dependent inhibitor of apoptosis-stimulating protein of p53 (iASPP) regulation was required in XIST-induced lung cancer cell growth. These findings indicated that XIST may regulate the tumor growth and metastasis via miR-140-dependent iASPP regulation. Taken together, our data indicated that XIST may be an oncogenic lncRNA that promotes the proliferation and metastasis of lung cancer through the regulation of miR-140 and could be regarded as a therapeutic target in human lung cancer.

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“…Recently, a close association between miRNAs and PDAC tumorigenesis has been elucidated [5,6]. Various miRNAs, such as miR-200 [7], miR-146a [8], miR-486 [9], and let-7 family [10] have been confirmed to be tumor suppressors.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-140 regulates cell growth and invasion in pancreatic duct adenocarcinoma by targeting iASPP

Liang

Gong

Zhang

et al. 2016

ABBS

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MicroRNAs are ∼22 nucleotide RNAs processed from RNA hairpin structures that play important roles in regulating protein expression level via binding to mRNA, either suppressing its translation or speeding up its degradation. In humans, they regulate most protein-coding genes, including genes important in cancer and other diseases. In this study, the expression of microRNA-140 (miR-140) was demonstrated to be significantly suppressed in pancreatic duct adenocarcinoma specimens and cell lines, compared with their adjacent normal tissues. With the help of bioinformatics analysis, inhibitor of apoptosis-stimulating protein of p53 (iASPP) was identified to be a direct target of miR-140, and luciferase reporter experiment confirmed this discovery. Overexpression of miR-140 decreases the protein expressions of iASPP, ΔNp63, MMP2, and MMP9. Growth and invasion of PANC-1 cells were attenuated by overexpression of miR-140 in vitro. The suppressive effect of miR-140 on PANC-1 cell line could be partly balanced out by manual overexpression of iASPP. Above all, these findings provided insights into the functional mechanism of miR-140, suggested that the miR-140/iASPP axis may interfere with the proliferative and invasive property of pancreatic duct adenocarcinoma cells, and indicated that miR-140 could be a potential therapeutic target for pancreatic duct adenocarcinoma.

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Differential expression profiling of microRNAs in para-carcinoma, carcinoma and relapse human pancreatic cancer

Abstract: The differential expressed miRNAs and their predicted target genes that involved in Jak-STAT signaling pathway, MAPK signaling pathway and PPAR signaling pathway indicating their potential roles in pancreatic carcinogenesis and progress.

Cited by 8 publications

References 26 publications

MiR-142 modulates human pancreatic cancer proliferation and invasion by targeting hypoxia-inducible factor 1 (HIF-1α) in the tumor microenvironments

MiR-142 modulates human pancreatic cancer proliferation and invasion by targeting hypoxia-inducible factor 1 (HIF-1α) in the tumor microenvironments

lncRNA XIST interacts with miR-140 to modulate lung cancer growth by targeting iASPP

MicroRNA-140 regulates cell growth and invasion in pancreatic duct adenocarcinoma by targeting iASPP

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