Sample Preincubation Strategy for Sensitive and Quantitative Detection of Clenbuterol in Swine Urine Using a Fluorescent Microsphere–Based Immunochromatographic Assay

Deng, Sheng; Shan, Shan; Xu, Chao; Liu, Dao F.; Xiong, Yong; Wei, Hua; Lai, Wei H.

doi:10.4315/0362-028x.jfp-14-086

Cited by 22 publications

(13 citation statements)

References 15 publications

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“…Therefore, the concentration of the RP should be optimized. Interestingly, the peak area ratio of test line to control line (P T /P C ) for evaluating miR-21 levels was more precious because it eliminated the effects of immunoreaction dynamics parameters (the efficiencies of antibody-antigen interactions on test line and control line) [ 34 ]. As shown in Fig 2 , background signals decreased as RP probe concentrations decreased from 80 to 50 nM.…”

Section: Resultsmentioning

confidence: 99%

Lateral flow nucleic acid biosensor for sensitive detection of microRNAs based on the dual amplification strategy of duplex-specific nuclease and hybridization chain reaction

Ying

Sun

et al. 2017

PLoS ONE

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MicroRNAs (miRNAs) constitute novel biomarkers for various diseases. Accurate and quantitative analysis of miRNA expression is critical for biomedical research and clinical theranostics. In this study, a method was developed for sensitive and specific detection of miRNAs via dual signal amplification based on duplex specific nuclease (DSN) and hybridization chain reaction (HCR). A reporter probe (RP), comprising recognition sequence (3’ end modified with biotin) for a target miRNA of miR-21 and capture sequence (5’ end modified with Fam) for HCR product, was designed and synthesized. HCR was initiated by partial sequence of initiator probe (IP), the other part of which can hybridize with capture sequence of RP, and was assembled by hairpin probes modified with biotin (H1-bio and H2-bio). A miR-21 triggered cyclical DSN cleavage of RP, which was immobilized to a streptavidin (SA) coated magnetic bead (MB). The released Fam labeled capture sequence then hybridized with the HCR product to generate a detectable dsDNA. This polymer was then dropped on lateral flow strip and positive result was observed. The proposed method allowed quantitative sequence-specific detection of miR-21 (with a detection limit of 2.1 fM, S/N = 3) in a dynamic range from 100 fM to 100 pM, with an excellent ability to discriminate differences in miRNAs. The method showed acceptable testing recoveries for the determination of miRNAs in serum.

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Section: Resultsmentioning

confidence: 99%

Lateral flow nucleic acid biosensor for sensitive detection of microRNAs based on the dual amplification strategy of duplex-specific nuclease and hybridization chain reaction

Ying

Sun

et al. 2017

PLoS ONE

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“…In the proposed format, the detected complexes are formed after the sample has rapidly moved along the tracks on the working membrane between neighboring zones, which are only 2 mm apart. Several immunochromatographic tests that exclude the conjugate pad have been reported [13][14][15]. In these assays, the conjugate is preincubated with the sample beyond the test strip.…”

Section: Discussionmentioning

confidence: 99%

Development of a Lateral Flow Highway: Ultra-Rapid Multitracking Immunosensor for Cardiac Markers

Byzova

Vengerov

Voloshchuk

et al. 2019

Sensors

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The integration of several controlled parameters within a single test system is experiencing increased demand. However, multiplexed test systems typically require complex manufacturing. Here, we describe a multiplexed immunochromatographic assay that incorporates a conventional nitrocellulose membrane, which is used together with microspot printing, to construct adjacent microfluidic "tracks" for multiplexed detection. The 1 mm distance between tracks allows for the detection of up to four different analytes. The following reagents are applied in separate zones: (a) gold nanoparticle conjugates with antibodies against each analyte, (b) other antibodies against each analyte, and (c) antispecies antibodies. The immersion of the test strip in the sample initiates the lateral flow, during which reagents of different specificities move along their tracks without track erosion or reagent mixing. An essential advantage of the proposed assay is its extreme rapidity (1-1.5 min compared with 10 min for common test strips). This assay format was applied to the detection of cardiac and inflammatory markers (myoglobin, D-dimer, and C-reactive protein) in human blood, and was characterized by high reproducibility (8%-15% coefficient of variation) with stored working ranges of conventional tests. The universal character of the proposed approach will facilitate its use for various analytes.

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“…Pre-incubation was successfully used in a competitive format of LFIA for the detection of veterinary drug residues (clenbuterol, sulfadiazine, and tetracycline) [ 14 ], the aptamer-based detection of β-conglutin [ 15 ] and aflatoxin B1 [ 16 ], and the simultaneous detection of five chemicals in drinking water [ 17 ]. Dentg et al showed that the LOD decreased by 3.5 times in clenbuterol detection (i.e., using five minutes pre-incubation) in comparison with the conventional LFIA [ 18 ]. Zvereva et al presented a LOD that decreased by 14 times in ractopamine detection [ 19 ], which confirmed the pre-incubating approach.…”

Section: Introductionmentioning

confidence: 99%

How to Improve Sensitivity of Sandwich Lateral Flow Immunoassay for Corpuscular Antigens on the Example of Potato Virus Y?

Razo

Panferov

Safenkova

et al. 2018

Sensors

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A simple approach was proposed to decrease the detection limit of sandwich lateral flow immunoassay (LFIA) by changing the conditions for binding between a polyvalent antigen and a conjugate of gold nanoparticles (GNPs) with antibodies. In this study, the potato virus Y (PVY) was used as the polyvalent antigen, which affects economically important plants in the Solanaceae family. The obtained polyclonal antibodies that are specific to PVY were characterized using a sandwich enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR). For LFIA, the antibodies were conjugated with GNPs with a diameter of 17.4 ± 1.0 nm. We conducted LFIAs using GNP conjugates in a dried state on the test strip and after pre-incubation with a sample. Pre-incubating the GNP conjugates and sample for 30 s was found to decrease the detection limit by 60-fold from 330 ng∙mL−1 to 5.4 ng∙mL−1 in comparison with conventional LFIA. The developed method was successfully tested for its ability to detect PVY in infected and uninfected potato leaves. The quantitative results of the proposed LFIA with pre-incubation were confirmed by ELISA, and resulted in a correlation coefficient of 0.891. The proposed approach is rapid, simple, and preserves the main advantages of LFIA as a non-laboratory diagnostic method.

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Sample Preincubation Strategy for Sensitive and Quantitative Detection of Clenbuterol in Swine Urine Using a Fluorescent Microsphere–Based Immunochromatographic Assay

Cited by 22 publications

References 15 publications

Lateral flow nucleic acid biosensor for sensitive detection of microRNAs based on the dual amplification strategy of duplex-specific nuclease and hybridization chain reaction

Lateral flow nucleic acid biosensor for sensitive detection of microRNAs based on the dual amplification strategy of duplex-specific nuclease and hybridization chain reaction

Development of a Lateral Flow Highway: Ultra-Rapid Multitracking Immunosensor for Cardiac Markers

How to Improve Sensitivity of Sandwich Lateral Flow Immunoassay for Corpuscular Antigens on the Example of Potato Virus Y?

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