Overcoming Concealment Effects of Targeting Moieties in the PEG Corona: Controlled Permeable Polymersomes Decorated with Folate‐Antennae for Selective Targeting of Tumor Cells

Yassin, Mohamed A.; Appelhans, Dietmar; Wiedemuth, Ralf; Formánek, Petr; Boye, Susanne; Lederer, Albena; Temme, Achim; Voit, Brigitte

doi:10.1002/smll.201402581

Cited by 64 publications

(100 citation statements)

References 55 publications

(52 reference statements)

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“…60 The exposure of the [PAH/PNMB11] 3 /PAH/PSS multilayers deposited on the silica particles under the unfiltered light of a mercury lamp for 30 min gave the desired cross‐linked multilayers on the surface of silica particles. By subsequent etching out the silica template by NH 4 F/HF buffer at pH 7.5, hollow capsules were smoothly obtained.…”

Section: Resultsmentioning

confidence: 99%

Photo‐Cross‐Linked Dual‐Responsive Hollow Capsules Mimicking Cell Membrane for Controllable Cargo Post‐Encapsulation and Release

et al. 2016

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Multifunctional and responsive hollow capsules are ideal candidates to establish highly sophisticated compartments mimicking cell membranes for controllable bio‐inspired functions. For this purpose pH and temperature dual‐responsive and photo‐cross‐linked hollow capsules, based on silica‐templated layer‐by‐layer approach by using poly(N‐isopropyl acrylamide)‐block‐polymethacrylate) and polyallylamine, have been prepared to use them for the subsequent and easily available post‐encapsulation process of protein‐like macromolecules at room temperature and pH 7.4 and their controllable release triggered by stimuli. The uptake and release properties of the hollow capsules for cargos are highly affected by changes in the external stimuli temperature (25, 37, or 45 °C) and internal stimuli pH of the phosphate‐containing buffer solution (5.5 or 7.4), by the degree of photo‐cross‐linking, and the size of cargo. The photo‐cross‐linked and dual stimuli‐responsive hollow capsules with different membrane permeability can be considered as attractive material for mimicking cell functions triggered by controllable uptake and release of different up to 11 nm sized biomolecules.

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Section: Resultsmentioning

confidence: 99%

Photo‐Cross‐Linked Dual‐Responsive Hollow Capsules Mimicking Cell Membrane for Controllable Cargo Post‐Encapsulation and Release

et al. 2016

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“…This is possible because the light scattering intensity is in direct relation with the molar mass. In this regard, the combination of AF4 or SEC with scattering techniques (see Figure 16) is a powerful tool for comprehensive characterisation of a wide range of polymers [194][195][196][197]. .…”

Section: Determination Principle and Requirementsmentioning

confidence: 99%

Size Separation Techniques for the Characterisation of Cross-Linked Casein: A Review of Methods and Their Applications

et al. 2018

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Abstract:Casein is the major protein fraction in milk, and its cross-linking has been a topic of scientific interest for many years. Enzymatic cross-linking has huge potential to modify relevant techno-functional properties of casein, whereas non-enzymatic cross-linking occurs naturally during the storage and processing of milk and dairy products. Two size separation techniques were applied for characterisation of these reactions: gel electrophoresis and size exclusion chromatography. This review summarises their separation principles and discusses the outcome of studies on cross-linked casein from the last~20 years. Both methods, however, show limitations concerning separation range and are applied mainly under denaturing and reducing conditions. In contrast, field flow fractionation has a broad separation range and can be easily applied under native conditions. Although this method has become a powerful tool in polymer and nanoparticle analysis and was used in few studies on casein micelles, it has not yet been applied to investigate cross-linked casein. Finally, the principles and requirements for absolute molar mass determination are reviewed, which will be of increased interest in the future since suitable calibration substances for casein polymers are scarce.

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“…Stimuli‐responsive (light, pH, temperature, and redox) disassembly or (partial) degradation of polymersomes has been established for potential therapeutic delivery of biologically active molecules. Release mechanisms of (macro)molecules from polymersomes without disassembly are also discussed which make use of change in the polymersome membrane permeability induced by light or by mechanical, physical, and chemical triggers . However, for the successful use of polymersomes in synthetic biology and in protocells, the interplay of other key characteristics is required.…”

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confidence: 99%

Dynamic Docking and Undocking Processes Addressing Selectively the Outside and Inside of Polymersomes

İyisan

Siedel

Gumz

et al. 2017

Macromol. Rapid Commun.

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Increasing complexity and diversity of polymersomes and their compartments is a key issue for mimicking cellular functions and protocells. Thus, new challenges arise in terms of achieving tunable membrane permeability and combining it with control over the membrane diffusion process, and thus enabling a localized and dynamic control of functionality and docking possibilities within or on the surface of polymeric compartments. This study reports the concept of polymersomes with pH-tunable membrane permeability for controlling sequential docking and undocking processes of small molecules and nanometer-sized protein mimics selectively on the inside and outside of the polymersome membrane as a further step toward the design of intelligent multifunctional compartments for use in synthetic biology and as protocells. Host-guest interactions between adamantane and β-cyclodextrin as well as noncovalent interactions between poly(ethylene glycol) tails and β-cyclodextrin are used to achieve selective and dynamic functionalization of the inner and outer spheres of the polymersome membrane.

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Overcoming Concealment Effects of Targeting Moieties in the PEG Corona: Controlled Permeable Polymersomes Decorated with Folate‐Antennae for Selective Targeting of Tumor Cells

Cited by 64 publications

References 55 publications

Photo‐Cross‐Linked Dual‐Responsive Hollow Capsules Mimicking Cell Membrane for Controllable Cargo Post‐Encapsulation and Release

Photo‐Cross‐Linked Dual‐Responsive Hollow Capsules Mimicking Cell Membrane for Controllable Cargo Post‐Encapsulation and Release

Size Separation Techniques for the Characterisation of Cross-Linked Casein: A Review of Methods and Their Applications

Dynamic Docking and Undocking Processes Addressing Selectively the Outside and Inside of Polymersomes

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