Phenotypic and genetic features of enteropathogenic <i>Escherichia coli</i> isolates from diarrheal children in the Ribeirão Preto metropolitan area, São Paulo State, Brazil

Pitondo-Silva, André; Nakazato, Gerson; Falcão, Juliana Pfrimer; Irino, Kinue; Martínez, Roberto; Darini, Ana Lúcia da Costa; Hernandes, Rodrigo T.

doi:10.1111/apm.12314

Cited by 14 publications

(18 citation statements)

References 43 publications

(47 reference statements)

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“…This finding is in agreement with several recent reports from Brazil and Iran of the occurrence of non-typeable EPEC serogroups (tEPEC and aEPEC) [49]. Most of the EPEC isolates did not belong to the classical EPEC O-serogroups, similar to what has been recently described in children from Iran and India [50,51].…”

Section: Discussionsupporting

confidence: 93%

“…Typical EPEC exhibited more resistance to the antimicrobials tested compared to the atypical EPEC. This is similar to reports from earlier studies [49,52]. Classification of MDR, based on published criteria, stipulates that a strain of EPEC is considered to be multidrug resistant if it is non-susceptible to at least three families of antibiotics [55].…”

Section: Discussionsupporting

confidence: 83%

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Molecular and phenotypic typing of enteropathogenic Escherichia coli isolated in childhood acute diarrhea in Abuja, Nigeria

Ifeanyi

Ikeneche

Bassey

et al. 2017

J Infect Dev Ctries

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Introduction: Enteropathogenic Escherichia coli (EPEC) causes infectious diarrhea among children in developing countries. However, in Nigeria, due to limited laboratory resources, the genetic diversity of its virulence factors, which include intimin subtypes, remains undefined. Methodology: EPEC isolates from diarrheic children 60 months of age and younger in Abuja, Nigeria, were analyzed. Polymerase chain reaction (PCR) for EPEC virulence gene, Hep-2 cell adherence, and serotyping were performed. EPEC strains were further subtyped by PCR for the identification of intimin subtype genes α (alpha), β (beta), γ1 (gamma-1), and έ (epsilon). Antibiotic resistance and extended-spectrum beta-lactamase (ESBL) production was determined by Clinical and Laboratory Standards Institute guidelines. Results: Overall, 18 (4.5%) out of 400 children with acute diarrhea had EPEC infection. Typical EPEC (tEPEC) strains were detected in 14 (3.5%), whereas 4 (1.1%) were atypical EPEC (aEPEC). A total of 15 (83.3%) of the EPEC isolated belonged to β intimin subtype gene, while the remaining 3 EPEC isolates possessed the intimin έ subtype. No α and γ intimin subtypes were detected. Traditional EPEC serotypes O114:H14 were detected only in tEPEC strains. Marked resistance to β-lactam agents were observed but no ESBL-producing tEPEC or aEPEC was detected. Conclusions: This is the first report of intimin subtype genes in Abuja, Nigeria. EPEC isolates of diverse serotypes resistant to β-lactam antimicrobial agents were observed. These data will be useful in facilitating the characterization of intimin variants of EPEC and some Shiga toxin-producing E. coli (STEC) in humans and other animal species.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 83%

Molecular and phenotypic typing of enteropathogenic Escherichia coli isolated in childhood acute diarrhea in Abuja, Nigeria

Ifeanyi

Ikeneche

Bassey

et al. 2017

J Infect Dev Ctries

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“…Apenas um marsupial foi identificado como portador da aEPEC, porém a sequência isolada apresentou 100% de similaridade com aquelas observadas em roedores. A grande diversidade genômica da E. coli lhe confere uma notável plasticidade ecológica; graças a ela, esses microrganismos adaptam-se rapidamente a diferentes ambientes, podendo, dessa forma, passar de organismo de vida livre a comensal do trato intestinal dos animais de sangue quente e, ainda, a patógenos que infectam humanos e animais 6,9,25,27,28,29,30 .…”

Section: Discussionunclassified

Caracterização molecular de Escherichia coli enteropatogênica atípica em animais silvestres capturados na Região Amazônica

Rocha¹,

Marinho²,

Santos³

et al. 2017

Revista Pan-Amazônica de Saúde

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“…En los estudios llevados a cabo en este campo se ha evidenciado la acentuada frecuencia con que se identifica el alelo β de la intimina en pacientes con cuadros diarreicos y en individuos aparentemente sanos, sin que se observe una correlación entre los serogrupos de E. coli y el tipo de intimina identificado (15,33,35).…”

Section: Discussionunclassified

“…En varios estudios a nivel mundial se ha demostrado que los patotipos de E. coli causante de diarrea usualmente están involucrados en la etiología de la diarrea en niños (3,10), con una elevada frecuencia de ECEP típicas y atípicas como agente patógeno único o asociado a otros agentes microbianos (11)(12)(13)(14)(15)(16). Asimismo, diversos estudios en Venezuela coinciden en señalar el importante papel de estas cepas en el síndrome diarreico agudo infantil, como se demostró en una investigación de 754 niños de Caracas, en la cual se determinó la significación epidemiológica de los aislamientos de E. coli con fenotipo de adherencia localizada en cultivos celulares (17).…”

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Identificación de Escherichia coli enteropatógena en niños con síndrome diarreico agudo del Estado Sucre, Venezuela

et al. 2016

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: concepción y diseño del estudio Adriana Millán, Mirian Michelli, Jesús Luiggi y Numirin Carreño: recolección y procesamiento de muestras Elvia Michelli: redacción del manuscrito Todos los autores participaron en el análisis y la interpretación de los datos. Materiales y métodos. Previo consentimiento informado, se recolectaron muestras fecales y se identificó E. coli mediante coprocultivo estándar y serología con antisueros polivalentes y monovalentes. Se aisló el ADN y se amplificaron los genes eae (intimina) y bfpA (bundlina) mediante dos pruebas de reacción en cadena de la polimerasa (PCR) múltiples. Identificación deResultados. En 39,6 % de los coprocultivos se determinó la presencia de infección bacteriana. La prevalencia de E. coli fue de 54,7 %; 82,9 % de estas cepas fue positivo por serología para los serogrupos y el serotipo evaluados, principalmente en niños entre los 0 y los 2 años (37,9 %). El 48,6 % de las cepas de E. coli amplificaron para el gen eae y, de estas, 58,8 % se clasificó como cepas de E. coli enteropatógena típica (eae+ y bfp+). El ECEP II fue el serogrupo más frecuente (38,7 %), con predominio de bacterias E. coli enteropatógenas típicas (60 %). El alelo β de la intimina fue el más identificado (74,5 %) en las cepas positivas para el gen eae. Solo se identificaron cuatro cepas con el serotipo O157:H7 utilizando antisueros, las cuales no amplificaron mediante PCR para los genes eae y bfpA. Conclusiones. Este estudio demostró la importancia de aplicar pruebas moleculares en la identificación de las cepas de E. coli causantes de diarrea de diversa gravedad. Materials and methods:After obtaining the informed consent, stool samples were collected. Escherichia coli was identified using standard coproculture methods and serology with polyvalent and monovalent antisera. DNA was isolated, and eae (intimin) and bfpA (bundlin) genes were amplified through two multiplex polymerase chain reactions (PCR). Results:The presence of bacterial infection was determined in 39.6% of coprocultures. The prevalence of E. coli was 54.7%; 82.9% of these isolates were positive by serology for the evaluated serogroups and serotypes, which were mostly identified in children between 0 and 2 years (37.9%); 48.6% of E. coli strains amplified the eae gene; of these, 58.8% were classified as typical EPEC (eae+ y bfp+). EPEC II was the most common serogroup (38.7%), with predominance of typical EPEC

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Phenotypic and genetic features of enteropathogenic Escherichia coli isolates from diarrheal children in the Ribeirão Preto metropolitan area, São Paulo State, Brazil

Cited by 14 publications

References 43 publications

Molecular and phenotypic typing of enteropathogenic Escherichia coli isolated in childhood acute diarrhea in Abuja, Nigeria

Molecular and phenotypic typing of enteropathogenic Escherichia coli isolated in childhood acute diarrhea in Abuja, Nigeria

Caracterização molecular de Escherichia coli enteropatogênica atípica em animais silvestres capturados na Região Amazônica

Identificación de Escherichia coli enteropatógena en niños con síndrome diarreico agudo del Estado Sucre, Venezuela

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