The Protective Effects of the Supercritical-Carbon Dioxide Fluid Extract of<i>Chrysanthemum indicum</i>against Lipopolysaccharide-Induced Acute Lung Injury in Mice via Modulating Toll-Like Receptor 4 Signaling Pathway

Wu, Xiaoli; Feng, Xue-Xuan; Li, Chuwen; Zhang, Xiaojun; Chen, Zhiwei; Chen, Jiannan; Lai, Xiaoping; Zhang, Saixia; Li, Yucui; Su, Zi-Ren

doi:10.1155/2014/246407

Cited by 26 publications

(22 citation statements)

References 51 publications

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“…We did not observe any significant decrease in the levels of ALU247; however DNA integrity decreased significantly after surgery which indicates more of apoptotic DNA in circulation. The higher levels of ALU115 observed in patients after surgery might be due to the increase in apoptosis associated with wound healing (Greenhalgh 1998; Wu and Chen 2014). The persistence of longer fragments of DNA after excision of tumor indicates that its presence in circulation might be associated with some other body mechanism.…”

Section: Discussionmentioning

confidence: 99%

Circulating cell-free DNA and its integrity as a prognostic marker for breast cancer

et al. 2015

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The aim of our study was to look for alternative predictive biomarkers for breast cancer management in limited resource setup. A comprehensive analysis of circulating cell-free DNA (CCFD) in serum at baseline was performed to assess its prognostic potential. Quantitative polymerase chain reaction (qPCR) of ALU sequences using ALU115 and ALU247 primers was carried out in patients (N: baseline 148, postoperative 47) and 51 healthy controls. Mean serum DNA integrity, levels of ALU 247 and levels of ALU 115 were significantly higher in patients than in healthy females. No significant differences were observed in the levels ALU 247 and ALU 115 between stage IV and earlier stages of the disease. The DNA integrity was significantly higher in stage IV than earlier stages. A significant decrease in DNA integrity was observed after surgery (pre: 0.55 ± 0.23 vs post: 0.43 ± 0.30; P = 0.002) while no such change could be observed for ALU 247 and ALU 115. Baseline DNA integrity was significantly higher in relapsed patients than in patients who were free of disease (P = 0.005). Higher baseline DNA integrity was also indicated, though statistically not significant, in patients who died (P = 0.14). In contrast, ALU 247 and ALU 115 levels were decreased in died patients as compared to survivors (24.8 ± 34.80 vs 73.5 ± 170.83, P = 0.02 for ALU 247 and 41.0 ± 47.99 vs 159.5 ± 299.54, P = 0.005 for ALU 115). Baseline levels of ALU 115 and ALU 247 were lower in relapsed patients, though statistically not significant. In univariate analysis, the only clinic-pathological parameter associated with disease prognosis was tumor size. The hazards of 5-year overall mortality was 3.60 (95 % CI: 1.03 12.53, P = 0.03) among patients with lower baseline serum levels of CCFD (ALU 247 < 21 and ALU 115 < 41). Similarly the 4 year hazards for recurrence was 2.30 (95 % CI: 0.96 5.52, P = 0.05) among patients with higher DNA integrity. Baseline serum levels of CCFD and its integrity were found to be potential prognostic biomarkers in patients of primary breast cancer at our centre.Electronic supplementary materialThe online version of this article (doi:10.1186/s40064-015-1071-y) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Circulating cell-free DNA and its integrity as a prognostic marker for breast cancer

et al. 2015

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“…20121104) [19] . According to our previous report, the composition analysis was done by combining Gas Chromatography-Mass Spectrometer (GC-MS) and high-performance liquid chromatography with Photodiode Array Detector (HPLC-PAD).…”

Section: Methodsmentioning

confidence: 99%

“…Pongjit et al reported that CI SCFE has a strong ability to protect against the chemotherapy-induced renal cell damage [17]. In addition, our previous study demonstrated that CI SCFE has a protective effect against lipopolysaccharide-induced lung injury and UV-induced skin injury [18,19]. However, the effective antitumor activity of CI SCFE combined with BLM in vivo remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Supercritical-Carbon Dioxide Fluid Extract from Chrysanthemum indicum Enhances Anti-Tumor Effect and Reduces Toxicity of Bleomycin in Tumor-Bearing Mice

Yang

Sun

Liang

et al. 2017

IJMS

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Bleomycin (BLM), a family of anti-tumor drugs, was reported to exhibit severe side effects limiting its usage in clinical treatment. Therefore, finding adjuvants that enhance the anti-tumor effect and reduce the detrimental effect of BLM is a prerequisite. Chrysanthemum indicum, an edible flower, possesses abundant bioactivities; the supercritical-carbon dioxide fluid extract from flowers and buds of C. indicum (CISCFE) have strong anti-inflammatory, anti-oxidant, and lung protective effects. However, the role of CISCFE combined with BLM treatment on tumor-bearing mice remains unclear. The present study aimed to investigate the potential synergistic effect and the underlying mechanism of CISCFE combined with BLM in the treatment of hepatoma 22 (H22) tumor-bearing mice. The results suggested that the oral administration of CISCFE combined with BLM could markedly prolong the life span, attenuate the BLM-induced pulmonary fibrosis, suppress the production of pro-inflammatory cytokines (interleukin-6), tumor necrosis factor-α, activities of myeloperoxidase, and malondiadehyde. Moreover, CISCFE combined with BLM promoted the ascites cell apoptosis, the activities of caspases 3 and 8, and up-regulated the protein expression of p53 and down-regulated the transforming growth factor-β1 by activating the gene expression of miR-29b. Taken together, these results indicated that CISCFE could enhance the anti-cancer activity of BLM and reduce the BLM-induced pulmonary injury in H22 tumor-bearing mice, rendering it as a potential adjuvant drug with chemotherapy after further investigation in the future.

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“…Thus, it can be referred as a potential therapeutic drug for ALI. Its mechanisms were at least partially associated with the modulations of TLR4 signaling pathways 55 . The oral intake of peptide mixture and the aqueous extract of Chrysanthemum Gui et al, 2014 morifolium had synergistic antimelanogenic and antioxidative effects in UV-irradiated mice 56 .…”

Section: Key Findings Referencementioning

confidence: 99%

An Up-to-Date Review of Phytochemicals and Biological Activities in Chrysanthemum spp

Chae¹

2016

Biosci., Biotech. Res. Asia

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The Chrysanthemum is a well-known traditional Chinese medicinal herb which has been used as a drug for thousands of years. From the detailed literature survey it is determined that the dried flowers of Chrysanthemum contain organic groups such as alkanes, flavonoids, terpinoids, unsaturated fatty acids and polysaccharides which belongs to Secondary constituents in Phytochemicals classification. The knowledge and analysis of bioactive compounds present in the Chrysanthemums can be used for next generation drug development process. The developments of new drugs against diabetes and prostate cancer have become global concern. Hence the present study leads to identify the new strategies which are needed to develop multi-functional drugs against various human diseases using Chrysanthemum plant. Owing to antifungal, antibacterial and anti-inflammation activities, Chrysanthemum have much attention in the field of biomolecules research. In general Phytochemicals can be classified as primary and secondary constituents. One can understand the fascinating properties of biological compounds via phytochemical analysis which are present in the plants. Therefore, the present study gives a better understanding of phytochemical properties of Chrysanthemum and new foundations for the production of non-toxic and eco-friendly drugs of today and the future.

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The Protective Effects of the Supercritical-Carbon Dioxide Fluid Extract ofChrysanthemum indicumagainst Lipopolysaccharide-Induced Acute Lung Injury in Mice via Modulating Toll-Like Receptor 4 Signaling Pathway

Cited by 26 publications

References 51 publications

Circulating cell-free DNA and its integrity as a prognostic marker for breast cancer

Circulating cell-free DNA and its integrity as a prognostic marker for breast cancer

Supercritical-Carbon Dioxide Fluid Extract from Chrysanthemum indicum Enhances Anti-Tumor Effect and Reduces Toxicity of Bleomycin in Tumor-Bearing Mice

An Up-to-Date Review of Phytochemicals and Biological Activities in Chrysanthemum spp

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