Doenjang prepared with mixed starter cultures attenuates azoxymethane and dextran sulfate sodium-induced colitis-associated colon carcinogenesis in mice

Abstract

Backgrounds:Doenjang is traditional Korean fermented soybean paste and widely known for its various health benefits including anticancer effect. In this study, we manufactured doenjang with the grain-type meju using probiotic mixed starter cultures of Aspegillus oryzae, Bacillus subtilis-SKm, and Lactococcus lactis-GAm to improve the qualities and beneficial properties of doenjang.Materials and Methods:The inhibitory effects of the doenjang prepared with the grain-type meju using mixed starter cultures were investigated in azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colon carcinogenesis mice model. AOM and DSS colon carcinogenesis was induced in female C57BL/6 mice, and doenjang was orally administered for 4 weeks. Body weight, colon length, and colon weight of mice were determined, and colonic tissues were histologically evaluated. The serum levels of proinflammatory cytokines as well as the expression of inflammation- and apoptosis-related genes in colonic tissue were also analyzed.Results:Administration of the doenjang using probiotic mixed starter cultures ameliorated the symptoms of colon cancer, and reduced the incidence of neoplasia, and reduced the levels of serum proinflammatory cytokines such as interleukin-6, and tumor necrosis factor-α and inducible nitric oxide synthase and cycloooxygenase-2 expression levels in colonic tissue. In addition, it increased Bax and reduced Bcl-2 expression levels and increased p21 and p53 expression in the colonic tissues.Conclusion:These findings indicate that the doenjang attenuated colon carcinogenesis induced by AOM and DSS by ameliorating the symptoms of colon cancer, reducing the occurrence of neoplasia, regulating proinflammatory cytokine levels, and controlling the expressions of inflammation- and apoptosis-related genes in the colonic tissue.

Cancer-protective effect of a synbiotic combination between Lactobacillus gasseri 505 and a Cudrania tricuspidata leaf extract on colitis-associated colorectal cancer

“…This recovery action of damaged colon cells following AOM/DSS treatment has been also reported in other publications. 36,38 Colonic inflammation has been suggested to be an important factor for developing IBD and even CRC. 39 Therefore, its prevention may be a good strategy for suppressing IBD and CRC in the colon.…”

Protective Effect of Gochujang on Inflammation in a DSS-Induced Colitis Rat Model

“…The gene expression of TNF-α, IL-6, and IL-1β was reduced in the GCJ group compared to the DSS group. In other colitis mouse models, TNF-α, IL-6, and IL-1β in serum and colon were reduced after administering doenjang , fermented soybean paste, and isoflavones such as daidzein and genistein, which are bioactive compounds of soybeans [ 9 , 20 , 21 , 38 , 39 ]. Some of the bioactive compounds of Gochujang made from meju (fermented soybean) are daidzein and genistein [ 12 , 16 ].…”

Regulation of Intestinal Inflammation by Soybean and Soy-Derived Compounds

“…Findings from the latter study suggest that riboflavin-producing lactic acid bacteria may serve as an effective anti-inflammatory therapy to promote mucosal integrity [ 263 ]. Fermented soybean pastes synthesized with probiotic species Aspegillus oryzae , Bacillus subtilis-SKm , and Lactococcus lactis-GAm has also been shown to inhibit both DSS-colitis and azoxymethane (AOM)-induced colon carcinogenesis [ 264 , 265 ].…”