Effect of aqueous leaf extract ofDalbergia sissooRoxb. on spermatogenesis and fertility in male mice

Abstract

Dalbergia sissoo treatment caused reversible suppression of spermatogenesis and fertility in P mice, without eliciting detectable toxic effects.

Citrus limonextract: possible inhibitory mechanisms affecting testicular functions and fertility in male mice

“…This may be similar to men that have exhibited symptoms of hypogonadism even in the presence of normal testosterone concentrations [Travison et al 2006]. Further, it is pertinent to mention that there is also a report of a marked decline in serum testosterone without an effect on libido in Parkes mice, e.g., after treatment with aqueous leaf extract of Dalbergia sissoo [Verma and Singh 2014]. The absence of any changes in the weights of the liver, spleen, kidney, adrenal gland, and brain, serum levels of ALT, AST, and creatinine, and in hematological parameters in C. limon-treated mice suggests that the treatment does not induce systemic toxic effect.…”

“…For evaluation of quantitative changes in spermatogenesis caused by Citrus treatment, germ cell number at stage VII of the spermatogenic cycle was determined [Verma and Singh 2014]. The crude count of different germ cells (spermatogonia A, preleptotene spermatocytes, pachytene spermatocytes and step 7 spermatids) was corrected by Abercrombie's formula [Abercrombie 1946;Russell et al 1990] and Sertoli cell nuclei were used as the reference structure.…”

“…To the best of our knowledge, however, there is no detailed report of the effect of C. limon on the male reproductive system. The present study describes the effect of 50% ethanolic leaf extract of C. limon on the reproductive organs and fertility of the Parkes (P) strain male mice, which we have been using for an animal model [Verma and Singh 2014]. We assessed various male reproductive end points such as sperm parameters, testicular histology, testosterone assay and fertility indices, activities of steroidogenic enzymes viz.…”

Chemical constituents and pharmacological effects of Dalbergia sissoo - A review

“…Libido in Dalbergia sissoo -treated males showed no change, but their fertility was markedly suppressed. By 56 days of treatment withdrawal, the alterations induced in fertility parameters were returned to control levels [114].…”

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“…sissoo has been traditionally used for different medicinal purposes to cure various types of diseases 16 and is reported as an folk medicine and is used mainly as aphrodisiac, expectorant, emesis, ulcers, leucoderma, dysentery, stomach troubles, skin diseases 17 , anti-diarrhoeal 18 , stimulation of new bone formation 19 , anti-resorptive 20 , antiinflammatory 21 , anti-fungal 11 , anti-bacterial, antihelminthic, bronchodilator, abortifacient, antipyretic, aphrodisiac, expectorant, analgesic 22 , astringent, bleeding, piles, varicose veins 4 , osteogenic 23 , reversible suppression of spermatogenesis 24 , in heart problems 25,26 , menstrual disorders 27 as gastroprotective 28 , during jaundice 29 and also to treat sore throats, syphilis, gonorrhea and also showed anti-oxidant activity 30 which is almost two times higher than Selenium and Vitamin E. The tribal peoples of Odisha use the extract of D. sissoo for the treatment of scalding of urine 31 . Antibiotics: Ampicillin and Norfloxacin antibiotic discs were used which was purchased from Himedia, India.…”