<i>Citrus limon</i>extract: possible inhibitory mechanisms affecting testicular functions and fertility in male mice

Singh, Nidhi; Singh, Shio Kumar

doi:10.3109/19396368.2015.1078422

Cited by 7 publications

(4 citation statements)

References 26 publications

(22 reference statements)

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“…The affected tubules in testes of mice after FSE treatment exhibited intraepithelial vacuolation, germ cell exfoliation, giant cell formation, degeneration of germ cells, and marginal condensation of chromatin in round spermatids. Such changes have also been shown in mice testes after treatment with Allamanda cathartica (Singh & Singh, 2008), Dalbergia sissoo (Verma & Singh, 2014) and Citrus limon (Singh & Singh, 2016). The results also showed a significant decrease in testis weight after FSE treatment, and a decrease in testicular weight is considered as an indication of morphological abnormalities that may have resulted from the FSE treatment (Grima et al, 2001).…”

Section: Discussionmentioning

confidence: 73%

Effect of Trigonella foenum‐graecum L. seed extract on the reproductive system of male mice and possible mechanism of its action on spermatogenesis

2022

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Fenugreek seed exhibits antidiabetic, antineoplastic, hepatoprotective, antidepressant and immunomodulatory properties. Fenugreek also causes antifertility effects in rodents. However, the impact of fenugreek seed on male reproduction and the possible mode of its action are not properly evaluated. Herein, we examined the effect of aqueous seed extract of fenugreek (FSE) and the possible mechanism of its action on male reproductive health in mice. Parkes mice were orally administered FSE (600 mg/kg body weight/day) or distilled water for 28 and 56 days, respectively. Various sperm parameters, histopathology, serum testosterone level and fertility indices were assessed. Furthermore, steroidogenic enzymes activities, oxidative status and germ cell dynamics in the testis were evaluated. Toxicological endpoints were also assessed. Treatment with FSE caused degenerative changes in the testis histoarchitecture. The treatment also affected various sperm parameters and concentrations of sialic acid and fructose in the epididymis and seminal vesicle, respectively. Fenugreek treatment also had negative impact on oxidative status and germ cell dynamics in the testis; fertility indices were also affected in female mice impregnated by the extract‐treated male mice, though libido of the treated male mice remained unaffected. Results show that treatment with FSE caused adverse effects on the male reproductive health and pregnancy outcome in Parkes mice.

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Section: Discussionmentioning

confidence: 73%

Effect of Trigonella foenum‐graecum L. seed extract on the reproductive system of male mice and possible mechanism of its action on spermatogenesis

2022

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“…This is not surprising because there appears absence of good correlation between serum testosterone levels and the sexual function as symptoms of hypogonadism have been noted in men even in the presence of normal testosterone concentrations (Travison et al ., ). Further, it is relevant to mention that there are also reports of marked decrease in serum level of testosterone with no effect on libido in P mice, for example, after treatment with aqueous leaf extract of Dalbergia sissoo (Verma & Singh, ) and 50% ethanolic leaf extract of Citrus limon (Singh & Singh, ). The absence of any alterations in the weights of the liver, spleen, kidney and adrenal glands, serum levels of ALT, AST and creatinine, and in haematological parameters in Mimusops‐treated mice suggests that the treatment does not produce systemic toxic effects.…”

Section: Discussionmentioning

confidence: 99%

Aqueous fruit extract ofMimusops elengicauses reversible suppression of spermatogenesis and fertility in male mice

Singh

2016

Andrologia

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Antifertility efficacy of oral administration of aqueous fruit extract of Mimusops elengi (200, 400 and 600 mg kg(-1) body weight/day for 35 days) was evaluated in Parkes strain male mice. Various reproductive end points such as histopathology, sperm parameters, testosterone level, haematology, serum biochemistry and fertility indices were assessed; activities of 3β- and 17β-hydroxysteroid dehydrogenases, and immunoblot expressions of StAR and P450scc in the testis were also assessed. Histologically, testes in Mimusops-treated mice showed nonuniform and diverse degenerative changes in the seminiferous tubules; both affected and normal tubules were observed in the same sections of testis. The treatment had adverse effects on testicular hydroxysteroid dehydrogenases and StAR and P450scc, serum level of testosterone and on motility, viability and number of spermatozoa in cauda epididymis. However, serum levels of alanine aminotransferase, aspartate aminotransferase and creatinine, and haematological parameters were not affected by the treatment. Also, libido was not affected in treated males, but their fertility was markedly suppressed. By 56 days of treatment withdrawal, the alterations caused in the above parameters recovered to control levels, suggesting that Mimusops treatment causes reversible suppression of spermatogenesis and fertility in Parkes mice. Further, there were no detectable signs of toxicity in treated males.

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“…The negative control slides were processed in a similar way without using TdT in TUNEL reaction solution. TUNEL‐positive apoptotic germ cells were counted in 20 tubular cross sections per testis from each animal in a group, and mean value of apoptotic cells in each group was presented (Singh & Singh, ).…”

Section: Methodsmentioning

confidence: 99%

An insight into the possible mechanisms of antispermatogenic action ofDalbergia sissooin male mice

Verma

Singh

2017

Andrologia

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This study investigated the possible mechanisms of antispermatogenic action of Dalbergia sissoo in Parkes male mice. Mice were orally administered aqueous leaf extract of Dalbergia sissoo (50 and 100 mg kg body weight day for 35 days) and various testicular indices such 3β- and 17β-hydroxysteroid dehydrogenase (HSD) activities, Western blot analyses of StAR, cytochrome P450scc and caspase-3, germ cell apoptosis by TUNEL, and lipid peroxidation and antioxidant enzymes activities were assessed. A significant increase in lipid peroxidation level and a marked decrease in activities of superoxide dismutase, catalase, 3β- and 17β-HSD were noted in the testis of Dalbergia-treated mice compared to controls. The treatment also had adverse effect on expression levels of StAR and cytochrome P450scc in the testis. There was an increase in the number of TUNEL-positive germ cells and in expression level of caspase-3 in testes of Dalbergia-treated mice, especially in those treated with 100 mg dose compared to controls. By 56 days of withdrawal therapy, the alterations induced in the above parameters recovered to control levels. Our results thus suggest that Dalbergia treatment interferes with steroidogenesis and produces oxidative stress in the testis, which may induce germ cell apoptosis leading to suppression of spermatogenesis.

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Citrus limonextract: possible inhibitory mechanisms affecting testicular functions and fertility in male mice

Cited by 7 publications

References 26 publications

Effect of Trigonella foenum‐graecum L. seed extract on the reproductive system of male mice and possible mechanism of its action on spermatogenesis

Effect of Trigonella foenum‐graecum L. seed extract on the reproductive system of male mice and possible mechanism of its action on spermatogenesis

Aqueous fruit extract ofMimusops elengicauses reversible suppression of spermatogenesis and fertility in male mice

An insight into the possible mechanisms of antispermatogenic action ofDalbergia sissooin male mice

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