Development of a capillary electrophoretic method for determination of plasma clearance of iohexol in dogs and cats

Abstract

Renal function can be monitored by estimation of the glomerular filtration rate (GFR) i.e. by measuring the clearance of iohexol. There is a lack of a validated capillary electrophoretic methodThe resolution between iohexol and the internal standard was decreased over 0.04 M, Figure 3. However, 0.06 M was chosen in the final method dueThe validation was performed in accordance with the guidelines [2] and included selectivity, accuracy, precision, There is a lack of a validated capillary electrophoretic method that covers the concentration range for full curve clearance estimate of iohexol (i.e. 18-2900 mg/L).However, 0.06 M was chosen in the final method due to the higher buffer capacity and the longer time between the first migration peak and the electro-osmotic flow. The method was found to be selective with a good accuracy and precision. The stability of clinical samplesThe mobility difference is decreased at higher ionic strengths. accuracy and precision. The stability of clinical samples was determined to be at least eight months (and calibrator and QC solutions spiked in plasma at least 18 months). A migration reversal was observed. Its pH was dependent on the ionic strength of the BGE, Figure 2 The individual iohexol concentrations are used to estimate the GFR by plotting the plasma concentration versus time and by calculating the AUC versus time. on the ionic strength of the BGE, Figure 2

Simple and fast analysis of iohexol in human serums using micro‐hydrophilic interaction liquid chromatography with monolithic column

“…Iohexol ( N , N ′‐bis(2,3‐dihydroxypropyl)‐5‐[ N ‐(2,3‐dihydroxypropyl‐acetamido‐2,4,6‐triiodo‐isophthalamide) (Fig. ) is currently a standard marker for determination of glomerular filtration rate due to its accurate estimation compared to other markers such as inulin or creatinine . Iohexol does not bind to serum proteins or metabolize in the body, it is stable and freely filtered through the glomerular membrane in the kidney without readsorption or tubular secretion .…”

“…) is currently a standard marker for determination of glomerular filtration rate due to its accurate estimation compared to other markers such as inulin or creatinine . Iohexol does not bind to serum proteins or metabolize in the body, it is stable and freely filtered through the glomerular membrane in the kidney without readsorption or tubular secretion . However high dose of iohexol might lead to adverse effects in organisms .…”

“…Reliable and accurate method is needed to control dose amount of iohexol and to determine iohexol clearance for evaluating renal function and diagnosing chronic kidney diseases (CKD) or evaluating risks of developing CKD. For analysis of iohexol in serums, several analytical methods have been developed including HPLC–UV , LC–MS/MS , LC–ICP‐MS , CE , inductively coupled plasma atomic emission spectroscopy (ICP‐AES) and X‐ray fluorescence spectroscopy (XRF) . LC was the most commonly used method for analysis of iohexol ; two chromatographic peaks of iohexol stereoisomers could be observed from common RP C 18 columns .…”

“…In serum, protein is a major part of the sample and can interfere with the analysis or causes clogging of chromatographic column. Several agents have been tested for protein precipitation in serum such as methanol , TFA , perchloric acid , zinc sulfate , and acetonitrile .…”

Simple equation for calculation of plasma clearance for evaluation of renal function without urine collection in rats

“…Many analytical techniques have been reported for the determination of iohexol in plasma, such as high performance liquid chromatography (HPLC)–UV, X‐ray fluorescence, HPLC–tandem mass spectrometry (MS/MS), and capillary electrophoresis, each of which has advantages and disadvantages . HPLC–UV methods are still the most common.…”