Simple equation for calculation of plasma clearance for evaluation of renal function without urine collection in rats

Abstract

To develop an equation for the evaluation of renal function in rats using three dilutions of plasma samples and to validate this method by comparison with a reference method. The investigation was conducted in Sprague-Dawley (SD) rats after delivery of three doses of iohexol, with blood samples collected before and after dosage using a quantitative blood collection method. Plasma iohexol concentrations were detected by high performance liquid chromatography (HPLC). The extraction recovery of iohexol from plasma was >97.30% and the calibration curve was linear (r = 0.9997) over iohexol concentrations ranging from 10 to 1000 µg/mL. The method had an RE of <9.310 and intra- and inter-day RSD of <5.137% and <3.693%, respectively. The plasma clearance values obtained from the equation correlated closely (r = 0.763) with those obtained using the reference method. The relatively correlation in the results obtained using the method under investigation and the reference method indicate that this new equation can be used for preliminary assessment of renal function in rats.

Measured GFR in murine animal models: review on methods, techniques, and procedures

“…Iohexol (Omnipaque™, GE Healthcare) is a non-radioactive, iodinated, non-ionic monomeric, and water-soluble molecule widely used as a contrast medium [ 24 , 32 ]. In comparison with the first generation of contrast agents, iohexol has lower osmolarity and toxicity and better safety.…”