Heterologous expression and solution structure of defensin from lentil Lens culinaris

Шенкарев, Захар О.; Gizatullina, Albina K.; Finkina, Ekaterina I.; Alekseeva, E. A.; Balandin, Sergey V.; Mineev, Konstantin S.; Arseniev, Alexander S.; Ovchinnikova, Tatiana V.

doi:10.1016/j.bbrc.2014.07.104

Cited by 21 publications

(9 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The role of protein dynamics in the functional activity of plant defensins is suggested by the fact that many functionally important residues are located in the loops [ 60 , 126 ]. Currently, the information on the dynamics of plant defensins comes mostly from NMR relaxation studies, which report that both the picosecond to the nanosecond and the microsecond to millisecond motions are present in plant defensins [ 47 , 48 , 50 , 51 , 60 , 108 , 126 ]. Note that in proteins the microsecond to millisecond motions are typically marked as the most functionally relevant [ 127 , 128 , 129 , 130 , 131 ].…”

Section: Dynamics Properties Of Plant Defensinsmentioning

confidence: 99%

“…Note that in proteins the microsecond to millisecond motions are typically marked as the most functionally relevant [ 127 , 128 , 129 , 130 , 131 ]. It is not surprising that in such small, disulfide-stabilized, compact molecules as plant defensins, overall, the microsecond to millisecond conformational dynamics is observed in similar regions, though the degree of mobility varies between different defensins [ 47 , 48 , 50 , 51 , 60 , 108 , 126 ]. In this regard, the defensin molecule can be visualized as consisting of two parts.…”

Section: Dynamics Properties Of Plant Defensinsmentioning

confidence: 99%

See 1 more Smart Citation

Plant Defensins from a Structural Perspective

Kovaleva

Bukhteeva

Kit

et al. 2020

IJMS

View full text Add to dashboard Cite

Plant defensins form a family of proteins with a broad spectrum of protective activities against fungi, bacteria, and insects. Furthermore, some plant defensins have revealed anticancer activity. In general, plant defensins are non-toxic to plant and mammalian cells, and interest in using them for biotechnological and medicinal purposes is growing. Recent studies provided significant insights into the mechanisms of action of plant defensins. In this review, we focus on structural and dynamics aspects and discuss structure-dynamics-function relations of plant defensins.

show abstract

Section: Dynamics Properties Of Plant Defensinsmentioning

confidence: 99%

Section: Dynamics Properties Of Plant Defensinsmentioning

confidence: 99%

Plant Defensins from a Structural Perspective

Kovaleva

Bukhteeva

Kit

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…It is demonstrated that local conditions are essential for a proper function of human antimicrobial peptides. While human HD-6, like hBD-1, is active only after disruption of disulfide bonds under reduced conditions [ 43 ], many other defensins in the folded form can directly recognize specific lipids in pathogen’s membranes [ 163 , 164 ]. During such a molecular recognition process, the flexible residues in the loop regions of these small defense proteins are found to be essential based on several structural studies [ 163 , 164 , 165 , 166 ].…”

Section: Perspectivesmentioning

confidence: 99%

Antimicrobial Peptides in 2014

et al. 2015

View full text Add to dashboard Cite

This article highlights new members, novel mechanisms of action, new functions, and interesting applications of antimicrobial peptides reported in 2014. As of December 2014, over 100 new peptides were registered into the Antimicrobial Peptide Database, increasing the total number of entries to 2493. Unique antimicrobial peptides have been identified from marine bacteria, fungi, and plants. Environmental conditions clearly influence peptide activity or function. Human α-defensin HD-6 is only antimicrobial under reduced conditions. The pH-dependent oligomerization of human cathelicidin LL-37 is linked to double-stranded RNA delivery to endosomes, where the acidic pH triggers the dissociation of the peptide aggregate to release its cargo. Proline-rich peptides, previously known to bind to heat shock proteins, are shown to inhibit protein synthesis. A model antimicrobial peptide is demonstrated to have multiple hits on bacteria, including surface protein delocalization. While cell surface modification to decrease cationic peptide binding is a recognized resistance mechanism for pathogenic bacteria, it is also used as a survival strategy for commensal bacteria. The year 2014 also witnessed continued efforts in exploiting potential applications of antimicrobial peptides. We highlight 3D structure-based design of peptide antimicrobials and vaccines, surface coating, delivery systems, and microbial detection devices involving antimicrobial peptides. The 2014 results also support that combination therapy is preferred over monotherapy in treating biofilms.

show abstract

“…They mentioned that turnip contain a high amount of glucosinolates especially gluconasturtiin which cause protection against pathogens, antimicrobial and anticancer activities in humans. Also Peptides of lentils seeds have antimicrobial potential agenist Lens culinaris and antifungal activity against Botrytis cinerea and Neurospora crassa (Shenkarev et al 2014;Pina-Pérez and Ferrús Pérez, 2018).…”

Section: Microbial Examination Of Different Chicken Luncheon Samples mentioning

confidence: 99%

“…The unsaturated essential fatty acids of lentils ranged from 77.5 % to 81.7%, total tocopherols was 37 -64 l g/g on DW and carotenoid ranged from 64 % to 78% and the2,2diphenyl-1-picrylhydrazyl (DPPH) activity of carotenoids and tocopherols were 0.4893 and 0.3259 g/g, respectively which contributed as a strong antioxidant activity Zhang, et al, (2014). Peptides of lentils seeds have antimicrobial potential agenist Lens culinaris and antifungal activity against Botrytis cinerea and Neurospora crassa (Shenkarev et al, 2014 ;Pina-Pérez and Ferrús Pérez, 2018). Turnip contain a high amount of glucosinolates especially gluconasturtiin which cause protection against pathogens, antimicrobial and anticancer activities in humans (Fahey et al, 2001;Zhang et al, 2008;Aires et al, 2009 andThiruvengadam et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Fatty and Amino Acids Profile, Sensory and Microbial Loud of Chicken Luncheon Prepared with Lentil Powder, Turnip Plant and Cauliflower

Aly

Morsy

2019

Journal of Food and Dairy Sciences

View full text Add to dashboard Cite

The aim of this study was to use some plants such as lentils (Lens culinaris), turnip plant (Brassica rapa) and cauliflower (Brassica oleracea) in processing of chicken luncheon to low cost of production and promote nutritional value. Treatments of chicken luncheon samples included: a) control luncheon (CL) Basal formula without any additional ingredients, b) Basal formula + lentils powder (T1), c) Basal formula + fresh turnip plant roots (T2), d) Basal formula + fresh cauliflower (T3) and f) (T4) Basal formula + lentils powder + fresh turnip plant roots + fresh cauliflower. Some parameter of chicken luncheon produced from different treatments included saturated and unsaturated fatty acids, amino acids, sensory attributes and microbial loud were evaluated. The total saturated fatty acids for oils extracted from CL, T1 and T2 treatments were 41.15, 40.15 and 40.18%, while the total unsaturated fatty acids amounted to 58.75, 59.83 and 59.80 %, respectively and the palmitic acid presented the predominant saturated fatty acids, while oleic acid was the highest unsaturated fatty acids. Leucine is the major essential amino acid in chicken luncheon treatments. It was 4.41 % for T2 sample and 4.49 % for T1 sample. Glutamic acid showed higher ratio of non-essential amino acid ranged between 8.97% (T2) and 9.75% (control sample). The results showed that additive lentils, turnip plant and cauliflower to chicken luncheon samples during its preparation decreased and retarded the growth of total molds & yeasts, total bacterial, psychrophilic bacteria and spore-forming bacteria of chicken luncheon samples during cold storage at 4 ± 1°C, hence T1 and T2 increase the shelf life of chicken luncheon samples to four months compared other samples (Three months).The applied additive from lentils and turnip plant also improved the appearance, color, texture, taste and odor of the chicken luncheon samples. It was concluded that the activity of lentils and turnip plant as natural antimicrobial assay to control microbial load of chicken luncheon samples, should be used as a food additive to improve the safety of chicken products.

show abstract

Heterologous expression and solution structure of defensin from lentil Lens culinaris

Cited by 21 publications

References 32 publications

Plant Defensins from a Structural Perspective

Plant Defensins from a Structural Perspective

Antimicrobial Peptides in 2014

Evaluation of Fatty and Amino Acids Profile, Sensory and Microbial Loud of Chicken Luncheon Prepared with Lentil Powder, Turnip Plant and Cauliflower

Contact Info

Product

Resources

About