Presence of osteoinductive factors in bovine colostrum

Mussano, Federico; Cusani, Alberto Bartorelli; Brossa, Alessia; Carossa, Stefano; Bussolati, Gianni; Bussolati, Benedetta

doi:10.1080/09168451.2014.896733

Cited by 8 publications

(5 citation statements)

References 37 publications

(36 reference statements)

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“…In vitro osteogenic differentiation was performed at the same conditions described above to run a series of assays aiming at revealing established bone markers, as described elsewhere [25, 26]. …”

Section: Methodsmentioning

confidence: 99%

Cytokine, Chemokine, and Growth Factor Profile Characterization of Undifferentiated and Osteoinduced Human Adipose-Derived Stem Cells

Mussano

Genova

Corsalini

et al. 2017

Stem Cells International

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Bone is the second most manipulated tissue after blood. Adipose-derived stem cells (ASCs) may become a convenient source of MSC for bone regenerative protocols. Surprisingly, little is known about the most significant biomolecules these cells produce and release after being osteoinduced. Therefore, the present study aimed at dosing 13 candidates chosen among the most representative cytokines, chemokines, and growth factors within the conditioned media of osteodifferentiated and undifferentiated ASCs. Two acknowledged osteoblastic cell models, that is, MG-63 and SaOs-2 cells, were compared. Notably, IL-6, IL-8, MCP-1, and VEGF were highly produced and detectable in ASCs. In addition, while IL-6 and IL-8 seemed to be significantly induced by the osteogenic medium, no such effect was seen for MCP-1 and VEGF. Overall SaOS-2 had a poor expression profile, which may be consistent with the more differentiated phenotype of SaOs-2 compared to ASCs and MG-63. Instead, in maintaining medium, MG-63 displayed a very rich production of IL-12, MCP-1, IP-10, and VEGF, which were significantly reduced in osteogenic conditions, with the only exception of MCP-1. The high expression of MCP-1 and VEGF, even after the osteogenic commitment, may support the usage of ASCs in bone regenerative protocols by recruiting both osteoblasts and osteoclasts of the host.

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Section: Methodsmentioning

confidence: 99%

Cytokine, Chemokine, and Growth Factor Profile Characterization of Undifferentiated and Osteoinduced Human Adipose-Derived Stem Cells

Mussano

Genova

Corsalini

et al. 2017

Stem Cells International

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“…In addition to this, bovine colostrum has a content of 40 times more intense than human colostrum (Şahal et al 2018). Various components other than Ig from colostrum can also be purified and used by using various biotechnological methods (Sacerdote et al 2013, Mussano et al 2014.…”

Section: Resultsmentioning

confidence: 99%

Holştayn İneklerde Bir Laktasyonda Üretilen Toplam Kolostral IgG’nin Araştırılması

Kara

Yağci

et al. 2023

Kocatepe Veterinary Journal

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Since dairy cows have greater milk production than beef cows, because of the dilution of IgG colostrum of dairy cows assumed to be of poorer quality compared with beef cows. The objective of the present study was to investigate quality of colostrum and total IgG produced in a lactation by Holstein cows. The average colostrum volume at the first seven milking were measured as 5.42 L, 6.73 L, 8.55 L, 9.26 L, 8.44 L, 9 L, 10.01 L respectively. The average total colostrum produced by cows in the first 3 days period was calculated as 57.41L. The average colostrum IgG concentration were calculated as 90.81 mg/ml, 68.67 mg/ml, 58.40 mg/ml, 37.33 mg/ml, 15.22 mg/ml, 10.7 0mg/ml, 5.9 mg/ml respectively for each milking. In conclusion, enough colostrum and IgG are produced in the first 3 days in Holstein cows for calf feeding. In addition to this, there is huge opportunity that excessive amount of IgG and colostrum could be processed for other by-products.

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“…The second step aimed at identifying a suitable concentration range of bovine colostrum for further assessing its potential protective action in a cell model of DEX-treated osteoblast MC3T3-E1. The concentrations selected were taken from Mussano et al [ 34 ] and were 0.1, 1.0, 2.5, and 5 mg/mL of culture media. After colostrum treatment for 24 h, cell viability was assessed using the MTT assay.…”

Section: Resultsmentioning

confidence: 99%

A Neuroprotective Bovine Colostrum Attenuates Apoptosis in Dexamethasone-Treated MC3T3-E1 Osteoblastic Cells

Martín‐Aragón

Bermejo-Bescós

Benedı́

et al. 2021

IJMS

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Glucocorticoid-induced osteoporosis (GIO) is one of the most common secondary forms of osteoporosis. GIO is partially due to the apoptosis of osteoblasts and osteocytes. In addition, high doses of dexamethasone (DEX), a synthetic glucocorticoid receptor agonist, induces neurodegeneration by initiating inflammatory processes leading to neural apoptosis. Here, a neuroprotective bovine colostrum against glucocorticoid-induced neuronal damage was investigated for its anti-apoptotic activity in glucocorticoid-treated MC3T3-E1 osteoblastic cells. A model of apoptotic osteoblastic cells was developed by exposing MC3T3-E1 cells to DEX (0–700 μM). Colostrum co-treated with DEX was executed at 0.1–5.0 mg/mL. Cell viability was measured for all treatment schedules. Caspase-3 activation was assessed to determine both osteoblast apoptosis under DEX exposure and its potential prevention by colostrum co-treatment. Glutathione reduced (GSH) was measured to determine whether DEX-mediated oxidative stress-driven apoptosis is alleviated by colostrum co-treatment. Western blot was performed to determine the levels of p-ERK1/2, Bcl-XL, Bax, and Hsp70 proteins upon DEX or DEX plus colostrum exposure. Colostrum prevented the decrease in cell viability and the increase in caspase-3 activation and oxidative stress caused by DEX exposure. Cells, upon colostrum co-treated with DEX, exhibited higher levels of p-ERK1/2 and lower levels of Bcl-XL, Bax, and Hsp70. Our data support the notion that colostrum may be able to reduce DEX-induced apoptosis possibly via the activation of the ERK pathway and modulation of the Hsp70 system. We provided preliminary evidence on how bovine colostrum, as a complex and multi-component dairy product, in addition to its neuroprotective action, may affect osteoblastic cell survival undergoing apoptosis.

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Presence of osteoinductive factors in bovine colostrum

Cited by 8 publications

References 37 publications

Cytokine, Chemokine, and Growth Factor Profile Characterization of Undifferentiated and Osteoinduced Human Adipose-Derived Stem Cells

Cytokine, Chemokine, and Growth Factor Profile Characterization of Undifferentiated and Osteoinduced Human Adipose-Derived Stem Cells

Holştayn İneklerde Bir Laktasyonda Üretilen Toplam Kolostral IgG’nin Araştırılması

A Neuroprotective Bovine Colostrum Attenuates Apoptosis in Dexamethasone-Treated MC3T3-E1 Osteoblastic Cells

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