High-resolution dual-modality photoacoustic ocular imaging

Abstract

We have developed a prototype ocular imaging system that integrates optical-resolution photoacoustic microscopy and high-frequency ultrasound imaging. The system can perform high-resolution ocular imaging from the anterior region down to the fundus. It has successfully imaged murine eyes in vivo, including iris, lens, retina, and retinal pigment epithelium. Our results demonstrate that this system shows strong potential for the diagnosis of ophthalmic diseases.

Photoacoustic Imaging in Oncology: Translational Preclinical and Early Clinical Experience

“…In this review, basics and general principles of photoacoustic imaging along with cancer-specific applications of photoacoustic imaging that can be readily translated to clinic were discussed. With the availability of commercial preclinical imaging systems along with the ability to build relatively inexpensive custom systems, an increase in photoacoustic imaging investigation rate is expected in the coming years for various clinical applications, including and beyond cancer detection and characterization (47,(116)(117)(118)(119)(120)(121)(122)(123) absorption was reported, implying that the mean optical absorption increases almost monotonically with the severity of cervical cancer.…”

Tutorial on photoacoustic tomography

“…With its multiparameter and multiscale imaging capability, PAT has been applied in many different disciplines, including cardiology, 77,124,125 dermatology, [126][127][128][129] oncology, 130-133 ophthalmology, 67,69,[134][135][136] gastroenterology, [36][37][38]40,[137][138][139][140] hematology, [141][142][143] and neurology. [144][145][146][147] In terms of imaging locations, PAT can be used for human breast imaging [148][149][150] and smallanimal imaging of the brain, 70,151,152 ear, [153][154][155] eye, [134][135][136] liver, 15 intestine, 156 and skin.…”

Three-dimensional Hessian matrix-based quantitative vascular imaging of rat iris with optical-resolution photoacoustic microscopy in vivo

“…As the laser focal depth is more than 300 μm below the sample surface to efficiently image iris vessels, and the numerical aperture is 0.1, the surface laser fluence is below 11 mJ∕cm 2 , which is within the American National Standards Institute safety limit. 24,35,36 To confirm that our imaging study caused no damage to the iris, the rat was euthanized and the imaged eyeball was removed after the completion of photoacoustic imaging to test for any damages. The 4% paraformaldehyde was used to fix the eyeball, followed by paraffin-cut sectioning and Masson's trichrome staining to prepare the sample for further optical microscopic observation (Axio Lab.A1, ZEISS, Gottingen, Germany).…”