Simultaneous detection of duck hepatitis A virus types 1 and 3, and of duck astrovirus type 1, by multiplex RT-PCR

Chen, Linlin; Ma, Mingjie; Zhang, Ruihua; Xu, Qian; XingKui, Si; Wang, Yu; Xie, Zhixun; Jiang, Shijin

doi:10.1007/s12250-014-3444-8

Cited by 14 publications

(12 citation statements)

References 14 publications

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“…In recent years, mixed infections of different DHAVs become common in eastern Asia Chen et al, 2013Chen et al, , 2014Soliman et al, 2015). Although reverse transcription-PCR (RT-PCR) has proved to be useful for differential diagnosis of the mixed infection of DHAVs Kim et al, 2008;Chen et al, 2013), a test for detecting DHAVs based on the serotype-specific mAbs would be a more convenient method for epidemiological investigation.…”

Section: Discussionmentioning

confidence: 99%

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Identification of a conserved neutralizing linear B-cell epitope in the VP1 proteins of duck hepatitis A virus type 1 and 3

Zhang

Zhou

Xin

et al. 2015

Veterinary Microbiology

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Section: Discussionmentioning

confidence: 99%

“…For a long time, DHAV-1 has been the most common and most virulent, worldwide serotype among the DHVs (Levine and Fabricant, 1950;Asplin, 1965). In recent years, the mixed infections of different DHAVs become common in eastern Asia Chen et al, 2013Chen et al, , 2014Soliman et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Identification of a conserved neutralizing linear B-cell epitope in the VP1 proteins of duck hepatitis A virus type 1 and 3

Zhang

Zhou

Xin

et al. 2015

Veterinary Microbiology

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“…Mixed infections of DHAV-1 and DHAV-3 are widespread in China's mainland [21]. In this study, after intramuscular vaccination in ducklings, the synthetic Pep1-KLH displayed the ability of eliciting strong specific antibody against DHAV-1 and DHAV-3 in sera (Figure 9a).…”

Section: Discussionmentioning

confidence: 66%

A Novel Peptide Isolated from a Phage Display Peptide Library Modeling Antigenic Epitope of DHAV-1 and DHAV-3

et al. 2020

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Duck hepatitis A virus (DHAV), the major pathogen of duck virus hepatitis (DVH), causes severe diseases that threaten the duck industry worldwide. The VP1 protein, a major structural protein of DHAV, is able to induce neutralizing antibody in ducks. The purpose of this study was to identify the antigenic mimotope of DHAV by phage display technology. A monoclonal antibody (mAb) 4E6 against DHAV-1 and DHAV-3 was prepared, and a phage library prepared with the PhD-12 Phage Display Peptide Library Kit was screened with the mAb. A novel peptide, 1GLTWKLPPSM10 was identified with high affinity to the mAb and could specifically block mAb 4E6 from binding DHAV-1 and DHAV-3. Animal tests confirmed that the immunization of ducklings with the mimotope could inhibit the virus proliferation and protect the ducklings from DVH. In summary, the neutralizing conformational mimotope 1GLTWKLPPSM10 might be a promising vaccine candidate for the prevention of DHAV infection.

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“…DAstV-1 infection causes fatal hepatitis to ducklings [13,18] and is ubiquitous in China [30,31]. As the most prevalent serotype, the overall mortality in 1-to 2-week-old ducklings infected by DAstV-1 was 50%, and livers in the affected ducklings displayed typical multiple and widespread hemorrhagic lesions [13,32].…”

Section: Discussionmentioning

confidence: 99%

Identification of a Type-Specific Epitope in the ORF2 Protein of Duck Astrovirus Type 1

Lan

Zhang

et al. 2019

Animals

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Duck astrovirus type 1 (DAstV-1) infection constitutes a cause of viral hepatitis in ducklings and little is known about the B-cell epitope of DAstV-1. In this study, a monoclonal antibody (mAb) 3D2 against open reading frame 2 (ORF2) protein of DAstV-1 was used to identify the possible epitope in the four serotypes of DAstV. The mAb 3D2 showed no neutralization activity to DAstV-1, and reacted with the conserved linear B-cell epitopes of 454STTESA459 in DAstV-1 ORF2 protein. Sequence analysis, dot blot assay, and cross-reactivity test indicated that the epitope peptide was highly conserved in DAstV-1 sequence and mAb 3D2 had no cross-reactivity with other DAstV serotypes. To the best of our knowledge, this is the first report about identification of the specific conserved linear B-cell epitope of DAstV-1, which will facilitate the serologic diagnosis of DAstV-1 infection.

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Simultaneous detection of duck hepatitis A virus types 1 and 3, and of duck astrovirus type 1, by multiplex RT-PCR

Cited by 14 publications

References 14 publications

Identification of a conserved neutralizing linear B-cell epitope in the VP1 proteins of duck hepatitis A virus type 1 and 3

Identification of a conserved neutralizing linear B-cell epitope in the VP1 proteins of duck hepatitis A virus type 1 and 3

A Novel Peptide Isolated from a Phage Display Peptide Library Modeling Antigenic Epitope of DHAV-1 and DHAV-3

Identification of a Type-Specific Epitope in the ORF2 Protein of Duck Astrovirus Type 1

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