Critical role of cellular cholesterol in bovine rotavirus infection

Cui, Jin; Fu, Xinliang; Xie, Jiexiong; Gao, Ming; Hong, Malin; Chen, Yao; Su, Shuo; Li, Shoujun

doi:10.1186/1743-422x-11-98

Cited by 16 publications

(18 citation statements)

References 42 publications

(54 reference statements)

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“…Second, the concentration of MβCD and cholesterol used in this study did not generate significant adverse effects on cell viability as shown by MTT (data not shown). Third, the drug concentrations and the protocols are similar to those described in studies involving other viruses [ 27 , 39 ]. Finally, co-localization studies showed that PRV VP7 protein and a recombinant GPI-anchor protein which has a predilection for lipid rafts, were localized to the lipid rafts in the plasma membranes of MA104 cells.…”

Section: Discussionmentioning

confidence: 99%

Cholesterol of lipid rafts is a key determinant for entry and post-entry control of porcine rotavirus infection

Dou

Han

et al. 2018

BMC Vet Res

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BackgroundLipid rafts are major structural components in plasma membranes that play critical roles in many biological processes including virus infection. However, few reports have described the relationship between lipid rafts and porcine rotavirus (PRV) infection. In this study, we investigated whether or not the locally high concentrations (3–5 fold) of cholesterol present in lipid rafts are required for PRV infection, and further examined which stages of the infection process are most affected.ResultsWhen cellular cholesterol was depleted by methyl-β-cyclodextrin (MβCD), PRV infectivity significantly declined in a dose-dependent manner. This inhibition was partially reversed upon reintroduction of cholesterol into the system. This was corroborated by the co-localization of PRV with a recombinant, GPI-anchored green fluorescent protein, which functioned as a marker for membranous regions high in cholesterol and indicative of lipid rafts. Changes in virus titer and western blot analyses indicated that depletion of cellular cholesterol with MβCD had no apparent effect on PRV adsorption; however, depletion of cholesterol significantly restricted entry and post-entry of PRV into the cell. Both points of inhibition were restored to near normal levels by the addition of exogenous cholesterol.ConclusionsWe conclude from these studies that membrane-based cholesterol and in particular that localized to lipid rafts, is an indispensable biomolecule for PRV infection, and that cholesterol-based control of the infection process takes place during entry and immediately post-entry into the cell.

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Section: Discussionmentioning

confidence: 99%

Cholesterol of lipid rafts is a key determinant for entry and post-entry control of porcine rotavirus infection

Dou

Han

et al. 2018

BMC Vet Res

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“…40 Cellular cholesterol was shown to be critical for bovine RV replication in MA104 cells at the stages of viral cell entry and virus assembly. 41 However, more work is needed to explore these interesting observations in-depth. Human intestinal enteroid cultures showed interaction of viroplasms with LDs 42 (Figure 2H, Ref.…”

Section: Further Research On Inhibition Of Rv Replication By Ld-distumentioning

confidence: 99%

Rotavirus replication and the role of cellular lipid droplets: New therapeutic targets?

Lever

Desselberger

2016

Journal of the Formosan Medical Association

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Rotaviruses (RVs) are a major cause of acute gastroenteritis in infants and young children worldwide. These viruses infect the villous epithelium of the small intestine. Part of their replication occurs in cytoplasmic inclusion bodies termed viroplasms. Viroplasms and the lipid droplets (LDs) of cellular organelles are known to interact both physically and functionally. Compounds interfering with the homoeostasis of LDs significantly decrease the production of infectious RV progeny. There is considerable scope for more detailed exploration of such compounds as potential antiviral agents for a disease for which at present no specific therapy exists.

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“…Подобные концентрационные зависимости, монотонные и немонотонные, можно заметить [39] 88.04, Внутренняя (плазменная) Фосфатидилэтаноламин [34,44] 124.01, 142.03 Внутренняя (плазменная) Холестерин [34,44] 369. 35 Приведем выводы, сделанные на основании полученных результатов.…”

Section: экспериментальная частьunclassified

“…Другое интересное использование эффекта экстракции холестерина из плазматических мембран может быть связано с регулируемым разрушением анионами полиоксометаллатов холестерин-сфингомиелиновых рафтов. Последние, как хорошо известно, играют роль преимущественных мест адсорбции и сборки вирионов [44] на поверхности клеток, в частности вирусов гриппа. Это один из множества общих, возможных, механизмов формирования противовирусной активности полиоксометаллатов.…”

Section: заключениеunclassified

Воздействие Наноразмерных Анионов Кремний-Молибденовой Кислоты На Плазматическую Мембрану Фибробластов Эмбриона Человека

Ковалевский¹,

Гулин²,

Lopatina³

et al. 2020

Rossijskie nanotehnologii

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Методом времяпролетной масс-спектрометрии вторичных ионов (ToF-SIMS) исследованы изменения состава плазматических мембран (ПМ) фибробластов эмбриона человека под воздействием наноразмерных анионов кремний-молибденовой кислоты (КМК). Измерены и интерпретированы зависимости масс-спектров основных липидов ПМ от концентрации КМК, коррелирующие с их послойными распределениями и со сродством холестерина к фосфолипидам. Обнаружен новый для биохимии клетки эффект – значительное снижение относительного содержания в ПМ холестерина и сфингомиелина под воздействием многозарядных анионов гетерополикислоты (ГПК). Для водных растворов КМК при концентрации С ~ 10мкМ/л и периода воздействия 48 ч обнаружено, что количество холестерина в ПМ снижается в 2–2.5 раза, при этом количество сфингомиелина уменьшается на 20–25%. Предложен новый механизм начального воздействия ГПК на плазматические мембраны, сводящийся к селективному травлению многозарядными анионами. Согласно предлагаемому механизму на первом этапе взаимодействия аниона полиоксометаллата с клеткой осуществляется экстракция из ПМ холестерина и сфингомиелина – основных регуляторов проницаемости и микровязкости ПМ. Как следствие возросшей проницаемости ПМ в клетках возможно ускорение жизненно важных трансмембранных и латеральных процессов.

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Critical role of cellular cholesterol in bovine rotavirus infection

Cited by 16 publications

References 42 publications

Cholesterol of lipid rafts is a key determinant for entry and post-entry control of porcine rotavirus infection

Cholesterol of lipid rafts is a key determinant for entry and post-entry control of porcine rotavirus infection

Rotavirus replication and the role of cellular lipid droplets: New therapeutic targets?

Воздействие Наноразмерных Анионов Кремний-Молибденовой Кислоты На Плазматическую Мембрану Фибробластов Эмбриона Человека

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