Rutin inhibits UVB radiation-induced expression of COX-2 and iNOS in hairless mouse skin: p38 MAP kinase and JNK as potential targets

Choi, Ki-Seok; Kundu, Joydeb Kumar; Chun, Kyung‐Soo; Na, Hye-Kyung; Surh, Young‐Joon

doi:10.1016/j.abb.2014.05.016

Cited by 87 publications

(59 citation statements)

References 56 publications

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“…Anti-inflammatory activity of P. macrocarpa pericarp extract also proven through decreasing of iNOS expression in mice colon, this results in line with Rostoka et al [22] and Choi et al [23] whom study flavonoid isolates, state that kaempferol, myricetin and rutin lowering iNOS protein expression in the livers of mice, and myricetin lowering nitric oxide production in rat liver. Besides kaempferol, myricetin and rutin, naringin also can inhibit the activation of iNOS enzyme and activation of NF-κB through barriers to degrades IκB and translocation of p65, a subunit of the protein NF-κB which undergo translocation into the cell nucleus when NF-κB regardless of IκB [24].…”

Section: Discussionsupporting

confidence: 77%

The Effect of Mahkota Dewa (Phaleria Macrocarpa) (Scheff.) Fruit Pericarp Extract on Inos in Mice Colon Intermittently-Induced by Dextran Sodium Sulfate

Kusmardi

Shavera

Estuningtyas

et al. 2017

Asian J Pharm Clin Res

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Objective: The objective of this research was to investigate the anti-inflammatory effect of Mahkota Dewa fruit pericarp extract (Phaleria macrocarpa) on inducible nitric oxide synthase (iNOS) in mice colon induced by dextran sodium sulfate (DSS). Method:The simplisia of P. macrocarpa pericarp was weighed (1000 g) and extracted by maceration process. The total yield of the ethanolic extract was 26.43%. Phytochemical screening was carried out for the detection of the phytoconstituents by simple qualitative methods. The antiinflammatory activity was performed by DSS-induced colitis model through assessment of hematoxylin-eosin staining and expression of iNOS by immunohistochemistry assay at four different doses, i.e., 650, 1250, 2500, and 5000 mg/kg. Swiss Webster male mice weighing 25-30 g were used for the study.Results: Inflammation score in dose 625, 1250, 2500, and 5000 mg/kg were 1.63, 1.43, 1.32, and 2.20, respectively. This result is significantly different (p=0.008) with DSS group that was 4.37. The results of iNOS optical density score in dose 625, 1250, 2500, and 5000 mg/kg were 1.21, 1.119, 1.22, and 1.37, respectively. This result was significantly different (p=0.000) with DSS group that was 2.24. Conclusion:Pericarp extract of P. macrocarpa fruit exhibited anti-inflammatory activity in the experimental model shown by suppressing the expression of inflammatory cell and iNOS.

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Section: Discussionsupporting

confidence: 77%

The Effect of Mahkota Dewa (Phaleria Macrocarpa) (Scheff.) Fruit Pericarp Extract on Inos in Mice Colon Intermittently-Induced by Dextran Sodium Sulfate

Kusmardi

Shavera

Estuningtyas

et al. 2017

Asian J Pharm Clin Res

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“…However, the observed decrease in the level of free PUFAs indicates that their metabolism is associated with the increase in UV-dependent ROS generation; this effect is also indicated by the enhanced activity of many other lipid metabolic enzymes, such as COXs and LOXs [65], [66], which enhance ROS-dependent as well as enzymatic lipid oxidation. Rutin significantly suppresses UVB-induced COX-2 and iNOS expression in human keratinocytes in vitro, as well as in mouse skin in vivo [67], [68]; therefore, its phospholipid protection results not only from its radical scavenging activity but also from its inhibition of enzymatic peroxidation [37], [69]. The time it took to reach the maximum increase in ROS generation, particularly after UVB irradiation, responds by enhancing lipid peroxidation and consequently generates products of PUFA fragmentation, such as the reactive aldehyde 4-HNE, which is metabolically active [45]; this corresponds with the time it took to reach the highest amount of LDH leakage from fibroblasts, which confirms damage to the fibroblast membrane structure and function.…”

Section: Discussionmentioning

confidence: 99%

Time-dependent effect of rutin on skin fibroblasts membrane disruption following UV radiation

et al. 2017

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Chronic exposure of the skin to solar UV radiation induces a number of biological alterations, including a redox imbalance; therefore, there is an urgent need for skin cells protective compounds. The aim of this study was to determine the effects of natural, previously extensively examined, polyphenol with antioxidant properties – rutin, on UV-induced skin fibroblasts membrane disruption. Accordingly, fibroblasts exposed to UVA and UVB irradiation were incubated with rutin (12 h before and/or up to 24 h after irradiation), and the structural and metabolic changes were examined. Rutin penetration through the fibroblast phospholipid bilayer was aided by UVA-induced bilitranslocase activity 2–4 h after irradiation, while UVB irradiation led to enhanced phospholipid peroxidation and higher membrane permeability to facilitate the interaction of rutin with phospholipids. Lipidomic analysis revealed that 4 h of rutin treatment also partially prevented UVA/B-induced increase in phosphatidylethanolamine and phosphatidylcholine level, as well as their membrane localization, which resulted in an enhanced zeta potential in the cells and liposomes. Moreover, rutin 2 h following irradiation, in a various degree, prevented the increased in phospholipase A2 activity and ROS generation, and partially protected against the reduction of arachidonic and linoleic acids level and the lipid peroxidation product 4-hydroxynonenal level increase. Rutin effectively prevented against decrease in glutathione peroxidase, glutathione and vitamins E and C activities/levels, particularly 2 h following UVA irradiation.In conclusion, highest skin fibroblasts membrane level of rutin occurred in 2–4 h following UVA/B-radiation results in its strongest effect on biomembrane structure and functions and cellular antioxidant system irrespective of the radiation type.

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“…Unique is the presence of rutin (flavonol quercetin-3-O-rutinoside), that was not found in any other cereals. Rutin decreases the capillary fragility, reduces the risk of atherosclerosis, and has antiinflammatory, antimutagenic, anticarcinogenic, antihemorrhagic, antioxidative, hypotensive effects (Ahmed et al, 2014;Benso et al, 2016;Choi et al, 2014). Buckwheat proteins have relatively low digestibility, what is probably the reason of positive activities against obesity, constipation, colon tumour, mammary tumour, hypercholesterolaemia.…”

Section: Introductionmentioning

confidence: 99%

Buckwheat cultivars — phenolic compounds profiles and antioxidant properties

Mikulajová

Šedivá

Hybenová

et al. 2016

Acta Chimica Slovaca

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Common buckwheat (Fagopyrum esculentum) and tartary buckwheat (Fagopyrum tataricum) cultivars originating in different world countries were investigated and compared for their quantitative and qualitative abundance of phenolics and flavonoids. Moreover, the antioxidant properties were tested using two different methods. The total phenolic and total flavonoid content ranged from 0.897 to 4.226 mg GAE g -1 dw and from 0.238 to 4.626 mg rutin g -1 dw, respectively. Flavonoids -rutin, quercetin, and hydroxybenzoic acids -gallic, protocatechuic, vanillic and syringic were identified and quantified. Rutin was the most abundant flavonoid and protocatechuic acid was the most abundant phenolic acid in evaluated cultivars. All cultivars showed significant antiradical properties, but their chelating activity was weak. The German cultivar of tartary buckwheat Lifago had significantly higher phenolic content and better antioxidant properties than other cultivars. The content of rutin was 24 times higher and free radicals scavenging activity about 70 % higher than the average value of other cultivars.

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Rutin inhibits UVB radiation-induced expression of COX-2 and iNOS in hairless mouse skin: p38 MAP kinase and JNK as potential targets

Cited by 87 publications

References 56 publications

The Effect of Mahkota Dewa (Phaleria Macrocarpa) (Scheff.) Fruit Pericarp Extract on Inos in Mice Colon Intermittently-Induced by Dextran Sodium Sulfate

The Effect of Mahkota Dewa (Phaleria Macrocarpa) (Scheff.) Fruit Pericarp Extract on Inos in Mice Colon Intermittently-Induced by Dextran Sodium Sulfate

Time-dependent effect of rutin on skin fibroblasts membrane disruption following UV radiation

Buckwheat cultivars — phenolic compounds profiles and antioxidant properties

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