Effect on interleukin‐1β and interleukin‐8 levels following use of fibrin sealant for periodontal surgery

Abstract

Background: Fibrin sealant (FS) is a biologically derived tissue adhesive for securing flaps. The aim of the present randomized controlled clinical trial was to compare early wound healing by assessing interleukin-1b (IL-1b) and interleukin-8 (IL-8) levels from gingival crevicular fluid (GCF) after using FS and suture for periodontal flap closure. Methods: Thirty selected quadrants in 15 periodontitis patients were randomly assigned to either a test (fibrining) or control group (suturing) for flap closure. IL-1b and IL-8 were assessed in GCF using enzyme-linked immunosorbent assay (ELISA) before and eight days after surgery. Patients were recalled at 7, 14, 21 days and 3 months after surgery for clinical assessment. Results: There was a statistically significant decrease in IL-1b (84.82 AE 77.18, 29.2 AE 21.97 pg/ll) and 21.82 AE 21.93 pg/ll) levels in the test side after fibrining while there was an increase in the control side (IL-1b 31.40 AE 16.82, 128.8 AE 45.14; IL-8 31.40 AE 16.82, 128.83 AE 45.14 pg/ll) (p < 0.05). The change in concentration of IL-1b and IL-8 following intervention correlated significantly in both the sites. Clinical parameters differed significantly only on the seventh day with less plaque and bleeding on the test sites. Conclusions: Fibrin sealant enhances early wound healing by reducing inflammation after periodontal flap surgery.

Efficacy of bubaline blood derived fibrin glue in silk ligature-induced acute periodontitis in Wistar rats

“…Periodontal flap closure using fibrin sealant results in a reduction of inflammatory cell accumulation but an increase in blood vessels in the wound area compared with the use of conventional sutures [ 21 ]. The application of fibrin in periodontal surgery reduces interleukin-1β (IL-1β) and IL-8 levels in gingival crevicular fluid [ 22 ]. In addition, the bleeding parameter is significantly decreased in those defects treated with fibrin sealant compared with controls [ 22 ].…”

“…The application of fibrin in periodontal surgery reduces interleukin-1β (IL-1β) and IL-8 levels in gingival crevicular fluid [ 22 ]. In addition, the bleeding parameter is significantly decreased in those defects treated with fibrin sealant compared with controls [ 22 ]. However, the supply of fibrinogen, a substrate for fibrin formation, is limited.…”

Effect of two post‐surgical cleansing protocols on early periodontal wound healing and cytokine levels following osseous resective surgery: A randomized controlled study

“…It has been demonstrated that evaluation of cytokine levels in wound fluids is a sensitive and specific assessment of inflammation . Specifically, mediators such as interleukin (IL)‐1β and IL‐8 are crucial for post‐surgical wound healing . IL‐1β induces periodontal inflammation, upregulates adhesion molecules on endothelial cells, stimulates chemokine production and induces the expression of other mediators that amplify the inflammatory response .…”

Editorial: Natural Products in Oral Health

“…Periodontal diseases which primarily include gingivitis and periodontitis are caused by the bacterial biofilm but mediated and modified by the host [ 5 ]. Studies on natural products for periodontal diseases have led to commercially available medication especially for gingivitis [ 5 , 6 ]. Natural products have many properties which can have an effect on the immune and inflammatory process during disease progression apart from inhibiting pathogenic bacteria [ 7 , 8 ].…”