Interplay of adiponectin, TNFα and insulin on gene expression, glucose uptake and PPARγ, AKT and TOR pathways in rainbow trout cultured adipocytes

Bou, Marta; Todorčević, Marijana; Rodríguez, J. Jacob; Capilla, Encarnación; Gutiérrez, Joaquím; Navarro, Isabel

doi:10.1016/j.ygcen.2014.05.005

Cited by 32 publications

(46 citation statements)

References 66 publications

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“…TNFa decreases PPARg expression in adipocytes in line with the anti-fat storage effects and anti-insulin actions of this cytokine described in relation to the lipid metabolism of mammals and fish [42,61]. The TNFa effects observed are also consistent with the previously observed inhibitory effects of this cytokine on trout adipocyte differentiation [21].…”

Section: Discussionsupporting

confidence: 87%

“…Nevertheless, fish adipose tissue has not been analyzed in these studies, whereas the presence of IGF-I receptors in trout adipocytes [14] and the specific IGF-binding previously characterized in trout adipose tissue have been demonstrated [41]. However, we have previously demonstrated that IGF-I stimulates the proliferation of trout-cultured preadipocytes and enhances glucose uptake in this primary cell culture [14,42]. In the present work, we showed that IGF-I reduces lipolysis in the same way as insulin in isolated trout adipocytes.…”

Section: Discussionmentioning

confidence: 99%

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Regulation of lipid metabolism and peroxisome proliferator-activated receptors in rainbow trout adipose tissue by lipolytic and antilipolytic endocrine factors

Cruz-García

Sánchez-Gurmaches

Monroy

et al. 2015

Domestic Animal Endocrinology

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Regulation of lipid metabolism and peroxisome proliferator-activated receptors in rainbow trout adipose tissue by lipolytic and antilipolytic endocrine factors

Cruz-García

Sánchez-Gurmaches

Monroy

et al. 2015

Domestic Animal Endocrinology

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“…Not surprisingly, these cellular signaling pathways are also activated in response to the treatment with anabolic hormones, which can be compared with a situation of abundance of nutrients. Thus, insulin treatment enhanced phosphorylation state of Akt in rainbow trout adipocytes (Bouraoui et al, 2010), adipose tissue (Polakof et al, 2011b), liver and muscle (Polakof et al, 2010b), and mTOR in adipocytes (Bou et al, 2014), whereas IGF-1 treatment enhanced the P-Akt/ Akt ratio in muscle of rainbow trout (Codina et al, 2008).…”

Section: Effects On Integrative Energy and Nutrient Sensors And Cellumentioning

confidence: 93%

Feeding rainbow trout with a lipid-enriched diet: effects on fatty acid sensing, regulation of food intake, and cellular signaling pathways

Librán-Pérez

Geurden

Dias

et al. 2015

Journal of Experimental Biology

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Using rainbow trout fed with low-fat or high-fat diets, we aimed to determine whether the response of food intake, mRNA abundance of hypothalamic neuropeptides involved in the metabolic regulation of food intake and fatty acid sensing systems in the hypothalamus and liver are similar to results previously observed when levels of specific fatty acids were raised by injection. Moreover, we also aimed to determine if the phosphorylation state of intracellular energy sensor 5′-AMP-activated protein kinase (AMPK), and proteins involved in cellular signaling such as protein kinase B (Akt) and target of rapamycin (mTOR) display changes that could be related to fatty acid sensing and the control of food intake. The increased levels of fatty acids in the hypothalamus and liver of rainbow trout fed with a high-fat diet only partially activated fatty acid sensing systems and did not elicit changes in food intake, suggesting that the fatty acid sensing response in fish is more dependent on the presence of specific fatty acids, such as oleate or octanoate, rather than to the global increase in fatty acids. We also obtained, for the first time in fish, evidence for the presence and function of energy sensors such as AMPK and proteins involved in cellular signaling, like mTOR and Akt, in the hypothalamus. These proteins in the hypothalamus and liver were generally activated in fish fed the high-fat versus low-fat diet, suggesting that cellular signaling pathways are activated in response to the increased availability of fatty acids.

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“…Protein extraction and Western blot analysis were performed as previously reported [29]. Twenty μg of protein were subjected to SDS-PAGE gel electrophoresis and Western blot analysis was performed using the appropriate antibodies for long-chain acyl-CoA synthetase (ACSL-1) (cat no 98925), proliferating cell nuclear antigen (PCNA) (cat no 7907), phosphoenolpyruvate carboxykinase (PEPCK) (cat no 32879), perilipin 2 (PLIN2) (cat no 32888), all from Cell Signaling Technology Inc (Beverly, MA) and PPARγ (PPARγ; cat no 7196; Santa Cruz Biotechnology).…”

Section: Methodsmentioning

confidence: 99%

Gene expression profile during proliferation and differentiation of rainbow trout adipocyte precursor cells

et al. 2017

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BackgroundExcessive accumulation of adipose tissue in cultured fish is an outstanding problem in aquaculture. To understand the development of adiposity, it is crucial to identify the genes which expression is associated with adipogenic differentiation. Therefore, the transcriptomic profile at different time points (days 3, 8, 15 and 21) along primary culture development of rainbow trout preadipocytes has been investigated using an Agilent trout oligo microarray.ResultsOur analysis identified 4026 genes differentially expressed (fold-change >3) that were divided into two major clusters corresponding to the main phases observed during the preadipocyte culture: proliferation and differentiation. Proliferation cluster comprised 1028 genes up-regulated from days 3 to 8 of culture meanwhile the differentiation cluster was characterized by 2140 induced genes from days 15 to 21. Proliferation was characterized by enrichment in genes involved in basic cellular and metabolic processes (transcription, ribosome biogenesis, translation and protein folding), cellular remodelling and autophagy. In addition, the implication of the eicosanoid signalling pathway was highlighted during this phase. On the other hand, the terminal differentiation phase was enriched with genes involved in energy production, lipid and carbohydrate metabolism. Moreover, during this phase an enrichment in genes involved in the formation of the lipid droplets was evidenced as well as the activation of the thyroid-receptor/retinoic X receptor (TR/RXR) and the peroxisome proliferator activated receptors (PPARs) signalling pathways. The whole adipogenic process was driven by a coordinated activation of transcription factors and epigenetic modulators.ConclusionsOverall, our study demonstrates the coordinated expression of functionally related genes during proliferation and differentiation of rainbow trout adipocyte cells. Furthermore, the information generated will allow future investigations of specific genes involved in particular stages of fish adipogenesis.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3728-0) contains supplementary material, which is available to authorized users.

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Interplay of adiponectin, TNFα and insulin on gene expression, glucose uptake and PPARγ, AKT and TOR pathways in rainbow trout cultured adipocytes

Cited by 32 publications

References 66 publications

Regulation of lipid metabolism and peroxisome proliferator-activated receptors in rainbow trout adipose tissue by lipolytic and antilipolytic endocrine factors

Regulation of lipid metabolism and peroxisome proliferator-activated receptors in rainbow trout adipose tissue by lipolytic and antilipolytic endocrine factors

Feeding rainbow trout with a lipid-enriched diet: effects on fatty acid sensing, regulation of food intake, and cellular signaling pathways

Gene expression profile during proliferation and differentiation of rainbow trout adipocyte precursor cells

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