Serum Biomarkers of Keshan Disease Assessed Using a Protein Profiling Approach Based on ClinProt Technique

Abstract

The etiology of Keshan disease (KD), an endemic myocardiopathy in regions of China, is largely unknown. To show the protein changes in serum from KD patients versus controls and idiopathic dilated cardiomyopathy (IDCM) and to search specific biological markers for differential diagnosis for KD. Serum of 65 patients with KD was compared with 29 patients with IDCM, 62 controls from KD areas and 28 controls from non-KD areas by ClinProt/MALDI-ToF technique. The genetic algorithm, quick classifier algorithm and supervised neural network algorithm methods were used to screen marker proteins and establish diagnostic model. Thirty-four differential peaks were identified in KD patients compared with the healthy controls from non-KD areas. Thirty-eight differentially peaks were identified in KD patients and controls from KD areas; and sixty-seven differentially peaks were identified in patients with KD and patients with IDCM. We believe that marker protein peaks screened in KD patients, healthy controls and IDCM patients may provide clues for the differential diagnosis and treatment of KD.

Identification of Biomarkers for Endometriosis Using Clinical Proteomics

“…The ClinProt system facilitates preparation and acquisition of complex proteomic profile patterns and their comparative analyses. [ 9 ] It can optimize searches for biomarkers. In the study, we used ClinProt technology to explore and identify biomarkers from serum of EM patients.…”

Preliminary quantitative proteomics analysis in chronic and latent Keshan disease by iTRAQ labeling approach

“…Therefore, prevention efforts could focus on the KD pathogenesis. Proteomic studies of KD have been conducted by two-dimensional gel electrophoresis, ClinProt Technique and microarrays [ 21 – 23 ]. Given meaningful proteins are masked by the high-abundant proteins (HAPs) in serum, LAPs detection is pivotal.…”

Keshan Disease: A Potentially Fatal Endemic Cardiomyopathy in Remote Mountains of China

“…Nutritionists have found that the mean Se contents in hair were <0.122 mg/kg in endemic areas and >0.200 mg/kg in non-endemic KD zones (38). In 2020, a study investigated serum Se levels in 571 individuals and found that levels in those living in KD-endemic areas were only 0.97 µmol/L, which was significantly lower than the levels in those living in non-endemic areas (1.01 µmol/L) (39,40). Further, results of urinary Se loading tests showed that the population in the affected areas was Se-poor (38).…”

“…Further, results of urinary Se loading tests showed that the population in the affected areas was Se-poor (38). The Se status of heart, liver, kidney, and muscle compared with that of individuals with KD was up to 10-fold lower (40,41). Importantly, support for the Se deficiency hypothesis came from the observation that long-term oral supplementation of an inorganic Se compound, selenite, was effective in reversing the disease in endemic areas (42).…”