Effects of alginate on frozen-thawed boar spermatozoa quality, lipid peroxidation and antioxidant enzymes activities

Hu, Jinghua; Geng, Gui; Li, Qingwang; Sun, Xiaofang; Cao, Hualin; Liu, Yawei

doi:10.1016/j.anireprosci.2014.04.007

Cited by 30 publications

(24 citation statements)

References 36 publications

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“…Moreover, alginate was found to be beneficial for freezing/thawing of boar sperm, showing increased antioxidant enzyme activities, such as superoxide dismutase and glutathione peroxidase, and decreased lipid peroxidation, with low levels of malondialdehyde in a dose-dependent response [57].…”

Section: Discussionmentioning

confidence: 99%

Transplantation of testicular tissue in alginate hydrogel loaded with VEGF nanoparticles improves spermatogonial recovery

Poels

Abou-Ghannam

Decamps

et al. 2016

Journal of Controlled Release

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Section: Discussionmentioning

confidence: 99%

Transplantation of testicular tissue in alginate hydrogel loaded with VEGF nanoparticles improves spermatogonial recovery

Poels

Abou-Ghannam

Decamps

et al. 2016

Journal of Controlled Release

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“…Microtubules are the main structure of sperm tail; the change of microtubules motor activity could influence the sperm motility, and on the other hand, the ATP binding process could provide energy for motility and survival of spermatozoa. Moreover, the oxidoreductase activity could influence the survival of spermatozoa (Hu et al., ). In addition, previous studies have also proved that the microtubules motor activity is related with spermatogenesis process, by regulating sertoli cell shape and function, mitotic and meiotic division of germ cells, and manchette and sperm flagella development and function (O'Donnell & O'Bryan, ).…”

Section: Discussionmentioning

confidence: 99%

miRNA and piRNA expression profiles of breeder cock testes detected by next‐generation sequencing

Guo

et al. 2016

Reprod Domestic Animals

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miRNAs are small non-coding regulatory RNAs that play key roles in diverse biological processes. In this study, we used the Solexa sequencing technique to profile miRNAs in breeder cock testes to illustrate their functions. A total of 663 co-expressed miRNAs and 3,180 co-expressed piRNAs were detected in three libraries. Based on Mir-X miRNA qRT-PCR, three miRNAs representing low, medium and high expression levels according to the sequencing results were selected randomly to validate the miRNAs' expression profiles. Results suggested that the miRNA expression profiles data could represent actual miRNA expression levels. Moreover, target genes prediction of the co-expressed miRNAs and further Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed, which revealed that some candidate miRNAs were involved in the regulation of the spermatogenesis process, spermatozoa function and testicular metabolism. In conclusion, we provided a useful resource for further elucidation of the miRNAs' regulatory role in spermatogenesis, contributing to a preliminary database for functional and molecular mechanistic studies in testicular metabolism, spermatogenesis and other testes functions.

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“…; Hu et al. ). Besides direct addition of antioxidants to extender, certain antioxidant supplements can be provided in feed to improve semen quality (Strzezek et al.…”

Section: Technologies For the Improvement Of Ai Dosementioning

confidence: 98%

New Approaches to Boar Semen Evaluation, Processing and Improvement

Šutovský

2015

Reprod Domestic Animals

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Contents The improvement of boar reproductive performance may be the next frontier in reproductive management of swine herd in Unites States, facilitated by better understanding of boar sperm function and by the introduction of new advanced instrumentation in the andrology field. Objective single ejaculate evaluation and individual boar fertility prediction may be possible by introducing automated flow cytometric semen analysis with vital stains (e.g. acrosomal integrity and mito‐potential), DNA fragmentation analysis and biomarkers (ubiquitin, PAWP, ALOX15, aggresome) associated with normal or defective sperm phenotypes. Measurement of sperm‐produced reactive oxygen species (ROS) is a helpful indicator of normal semen sample. Semen ROS levels could be managed by the addition of ROS‐scavenging antioxidants. Alternative energy regeneration substrates and sperm stimulants such as inorganic pyrophosphate and caffeine could increase sperm lifespan in extended semen and within the female reproductive system. Such technology could be combined with timed sperm release in the female reproductive system after artificial insemination. Sperm phenotype analysis by the image‐based flow cytometry will go hand in hand with the advancement of swine genomics, linking aberrant sperm phenotype to the fertility influencing gene polymorphisms. Finally, poor‐quality ejaculates could be rescued and acceptable ejaculates improved by semen purification methods such as the nanoparticle‐based semen purification and magnetic‐activated sperm sorting. Altogether, these scientific and technological advances could benefit swine industry, provided that the challenges of new technology adoption, dissemination and cost reduction are met.

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Effects of alginate on frozen-thawed boar spermatozoa quality, lipid peroxidation and antioxidant enzymes activities

Cited by 30 publications

References 36 publications

Transplantation of testicular tissue in alginate hydrogel loaded with VEGF nanoparticles improves spermatogonial recovery

Transplantation of testicular tissue in alginate hydrogel loaded with VEGF nanoparticles improves spermatogonial recovery

miRNA and piRNA expression profiles of breeder cock testes detected by next‐generation sequencing

New Approaches to Boar Semen Evaluation, Processing and Improvement

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