Optimization of Extraction and Enrichment of Steroidal Alkaloids from Bulbs of CultivatedFritillaria cirrhosa

Abstract

The bulbs of cultivated Fritillaria cirrhosa (BCFC) are used in China both for food and folk medicine due to its powerful biological activities. The aim of this study is to optimize the extraction and enrichment conditions of alkaloids from BCFC. Firstly, the orthogonal experimental design was used to optimize and evaluate four variables (ethanol concentration, solid-liquid ratio, extraction time, and temperature). Thereafter, resin adsorption was as a means to enrich alkaloids. Among 16 tested resins, H-103 resin presented higher adsorption capacity and desorption ratio. The equilibrium experimental data of the adsorption of total alkaloids, imperialine, and peimisine were well-fitted to the pseudo-first-order kinetics model, Langmuir and Freundlich isotherms models. Finally, in order to optimize the parameters for purifying alkaloids, dynamic adsorption and desorption tests were carried out. After one run treatment with H-103 resin, the contents of total alkaloids, imperialine, and peimisine in the product were 21.40-, 18.31-, and 22.88-fold increased with recovery yields of 94.43%, 90.57%, and 96.16%, respectively.

Plant Resource Availability of Medicinal Fritillaria Species in Traditional Producing Regions in Qinghai-Tibet Plateau

“…Because production of BFC obtained from the wild field has fallen short of consumption and the price is growing higher in recent decades, the artificial cultivation of BFC has been gradually developed by the traditional Chinese medicinal cultivation companies (Wang et al, 2014a ). In this work, we investigated six artificial cultivated bases and two individual cultivated plantations (Figure 4 ).…”

“…Previous studies indicated that chemical constituents of BFC mainly include steroidal alkaloids, saponins, terpenoids, and glycosides (Hao et al, 2013 ). It is believed that alkaloids are the major biological active constituents (Li et al, 2013 ; Wang et al, 2014a ). Recent pharmacological studies found that alkaloids from BFC exhibit remarkable antitussive, expectorant, anti-inflammatory effects (Wang et al, 2011 , 2012 ), antiasthmatic effect (Yan, 2005 ), hypotensive property (Kang et al, 2004 ), anti-bacterial activity (Li et al, 2005 ), and antitumor effect (Wang D. D. et al, 2014 ; Wang et al, 2014b , 2015 ).…”

“…At present, BFC is mainly harvested from the wild fields. Production of BFC has fallen short of consumption (Wang et al, 2014a ). The price of BFC was beyond $260 per kilogram in 2014, almost nine-folds of that in 2004.…”

Evaluation of antitumor property of extracts and steroidal alkaloids from the cultivated Bulbus Fritillariae ussuriensis and preliminary investigation of its mechanism of action

“…So, the CE was subjected to further purification to get total alkaloids of BFU (TAFU). TAFU was prepared by using previous methods [ 15 ]. Simply, the CE was dissolved and chromatographed over H-103 resin column, which was eluted with distilled water, 10% alcohol and 90% ethanol, respectively.…”

A Systematic Review on Traditional Uses, Sources, Phytochemistry, Pharmacology, Pharmacokinetics, and Toxicity of Fritillariae Cirrhosae Bulbus

“…As the fundamental components of FCB, alkaloids are traditionally believed to be responsible for the pharmacological activities of FCB [ 34 ] and have been considered as a key index for the identification and quality control of FCB [ 36 ]. Interestingly, the content of total alkaloids in FCB is extremely low, approximately ranging from 0.02% to 0.3% [ 40 ]. Previous studies showed that the alkaloids from FCB could be classified into two groups based on the carbon framework: isosteroidal alkaloids and steroidal alkaloids; the former accounts for approximately 75% of the total alkaloids [ 18 , 41 ].…”