Resistance to β-lactam antibiotic may influence nanH gene expression in Trueperella pyogenes isolated from bovine endometritis

Zhang, Dexian; Kai, Tetsuya; Han, Limei; Wang, Qiuxia; Liu, Yaochuan; Tian, Chunlian; Ming-chun, Liu

doi:10.1016/j.micpath.2014.04.006

Cited by 13 publications

(9 citation statements)

References 21 publications

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“…The prevalence of T. pyogenes isolation may differ, depending on a host species and a geographic region. The majority of published data concerns T. pyogenes infections in food animals and comes from Europe [21,33,34,35,36,37,38], China [39,40,41,42,43], Japan [44,45], Brazil [30,46], and the United States [47,48,49,50]. In livestock, the diseases caused by T. pyogenes generate significant economic losses, mainly in cattle and swine breeding, causing a reduction of meat and milk yield, as well as decreased reproductive efficiency and sometimes the necessity to cull diseased animals.…”

Section: Pathogenicitymentioning

confidence: 99%

Pathogenicity and Virulence of Trueperella pyogenes: A Review

Rzewuska

Kwiecień

Chrobak-Chmiel

et al. 2019

IJMS

122

110

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Bacteria from the species Trueperella pyogenes are a part of the biota of skin and mucous membranes of the upper respiratory, gastrointestinal, or urogenital tracts of animals, but also, opportunistic pathogens. T. pyogenes causes a variety of purulent infections, such as metritis, mastitis, pneumonia, and abscesses, which, in livestock breeding, generate significant economic losses. Although this species has been known for a long time, many questions concerning the mechanisms of infection pathogenesis, as well as reservoirs and routes of transmission of bacteria, remain poorly understood. Pyolysin is a major known virulence factor of T. pyogenes that belongs to the family of cholesterol-dependent cytolysins. Its cytolytic activity is associated with transmembrane pore formation. Other putative virulence factors, including neuraminidases, extracellular matrix-binding proteins, fimbriae, and biofilm formation ability, contribute to the adhesion and colonization of the host tissues. However, data about the pathogen–host interactions that may be involved in the development of T. pyogenes infection are still limited. The aim of this review is to present the current knowledge about the pathogenic potential and virulence of T. pyogenes.

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Section: Pathogenicitymentioning

confidence: 99%

Pathogenicity and Virulence of Trueperella pyogenes: A Review

Rzewuska

Kwiecień

Chrobak-Chmiel

et al. 2019

IJMS

122

110

View full text Add to dashboard Cite

show abstract

“…The reverse transcription of the ompF mRNA was carried out by the PrimeScript RT reagent Kit (Takara). The mRNA expression of ompF was measured by real-time PCR (RT-PCR) as previously described (Zhang et al, 2014). Briefly, standard curves for RT-PCR were prepared by serially diluting the plasmids as the template for the ompF.…”

Section: Real-time Fluorescent Quantitative Pcrmentioning

confidence: 99%

The phylogenetic group, antimicrobial susceptibility, and virulence genes of Escherichia coli from clinical bovine mastitis

Zhang¹,

Zhang²,

Huang

et al. 2018

Journal of Dairy Science

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Bovine mastitis is still a central problem on dairy farms despite control programs, and Escherichia coli is a crucial pathogen during the development of bovine mastitis. The virulence genes, antimicrobial susceptibility, and mortality of mice infected with different E. coli isolates from bovine mastitis were determined in this study. According to the presence of the specific genes chuA, yjaA, and TspE4.C2, these isolates mainly belonged to 2 different groups: group A (47/79) and group B1 (22/79). The ompC gene was detected in all the isolates, followed by fimH (89.9%), ECs3703 (88.6%), and ompF (73.4%), whereas most of the virulence genes were not detected in these isolates. The results of the antimicrobial susceptibility tests indicated that the isolates were susceptible to the fluoroquinolones and aminoglycosides. An inverse relationship was shown between the expression level of ompF and antimicrobial resistance; additionally, the isolates that were nonsusceptible to at least 4 classes of antimicrobial agents showed a lower mortality to mice in comparison with the susceptible isolates. This study indicated that antibiotic resistance had emerged in E. coli from bovine mastitis in this area, and appropriate measures should be taken to avoid potential threats to humans and other animals.

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“…Bacterial biofilms, embedded in a self-produced polymer matrix consisting of polysaccharide, protein, and DNA, show an increased tolerance to antibiotics and disinfectants as well as resistance to phagocytosis and other components of the immune system; therefore, they result in chronic infections (Høiby et al, 2010). Previous studies showed that the intramammary administration of antibiotics against T. pyogenes infections was ineffective (Jousimies-Somer et al, 1996), whereas prolonged and intensive systemic treatment of early cases with beta-lactams and rifampin was occasionally effective (Hirvonen et al, 1994;Andrews et al, 2004;Zhang et al, 2014).…”

mentioning

confidence: 99%

Properties and antimicrobial susceptibility of Trueperella pyogenes isolated from bovine mastitis in China

Alkasir

Wang

Gao

et al. 2016

Acta Veterinaria Hungarica

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Trueperella (T.)pyogenes is an opportunistic pathogen that causes suppurative diseases in domestic animals. In this work, the properties, pathogenesis and phenotypic diversity of T. pyogenes isolates from bovine mastitis were studied. Both pyolysin (plo) and collagen-binding protein (cbp) virulence factor genes were detected by PCR in all T. pyogenes isolates (n = 50). Using the tissue culture plate method, 90% of T. pyogenes isolates were able to form biofilms. The minimum inhibitory concentrations (MICs) of 13 antimicrobials against T. pyogenes isolates were determined. High susceptibility was observed to rifampin (96%), ampicillin (94%), ciprofloxacin (94%), and penicillin (92%), while low susceptibility was found to trimethoprim-sulphamethoxazole (10%) and bacitracin (2%). The intracellular assay revealed that T. pyogenes isolates had different cytopathogenic effects on cells. The high percentage (28.6%) of T. pyogenes isolates suggests that this bacterium is an important contributor to mastitis. Moreover, the high occurrence of multidrug resistance, biofilm production, intracellular survival, and the temporal dynamics of T. pyogenes interactions are key factors for a better understanding of how immunity acts on infections with these bacteria and how they evade immune surveillance, thus highlighting the need for the prudent use of antimicrobial agents in veterinary medicine.Key words: Trueperella pyogenes, bovine mastitis, biofilm, virulence factor, antimicrobial susceptibility Mastitis is an inflammation of the mammary gland caused by several varieties of bacteria such as Staphylococcus (S.) aureus, S. epidermidis, and strep- † Equal contributors

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Resistance to β-lactam antibiotic may influence nanH gene expression in Trueperella pyogenes isolated from bovine endometritis

Cited by 13 publications

References 21 publications

Pathogenicity and Virulence of Trueperella pyogenes: A Review

Pathogenicity and Virulence of Trueperella pyogenes: A Review

The phylogenetic group, antimicrobial susceptibility, and virulence genes of Escherichia coli from clinical bovine mastitis

Properties and antimicrobial susceptibility of Trueperella pyogenes isolated from bovine mastitis in China

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