Chimaphilin induces apoptosis in human breast cancer MCF-7 cells through a ROS-mediated mitochondrial pathway

Ma, Weidong; Zou, Yu; Wang, Peng; Yao, Xiaohui; Sun, Yanfang; Duan, Minghui; Fu, Yujie; Yu, Bo

doi:10.1016/j.fct.2014.04.014

Cited by 54 publications

(28 citation statements)

References 36 publications

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“…For mitochondrial apoptosis pathway, ROS could directly activate the mitochondrial permeability transition and result in the loss of mitochondrial membrane potential (MMP) and the membrane integrity [12]. Subsequently, cytochrome c is released from mitochondria into cytosol and activates the caspase-cascades system, which eventually contributes to tumor cell apoptosis [13].…”

Section: Introductionmentioning

confidence: 99%

Seleno-short-chain chitosan induces apoptosis in human breast cancer cells through mitochondrial apoptosis pathway in vitro

Zhao

et al. 2018

Cell Cycle

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Seleno-short-chain chitosan (SSCC) was a synthesized chitosan derivative with the molecular weight of 4826.986 Da. The study is aimed to investigate cytotoxicity of SSCC on human breast cancer MCF-7 and BT-20 cells and explore apoptosis-related mechanism in vitro. The MTT (3- [4,5-Dimethylthiazol-2-yl]-2, 5-diphenylterazolium bromide) assay showed that SSCC exhibited significantly cytotoxic effects on MCF-7 and BT-20 cells in a dose- and time-dependent manner, and the effective inhibitory concentration was 100 μg/ml and 200 μg/ml, respectively. Apoptosis assay of these two kinds of cells was determined by Hoechst 33,342/PI and Annexin V-FITC/PI double staining. The cell cycle assay showed that SSCC triggered S and G2/M phase cell cycle arrest in MCF-7 cells and S phase cell cycle arrest in BT-20 cells in a time-dependent manner. Further studies demonstrated that SSCC led to the generation of reactive oxygen species (ROS) and the disruption of mitochondrial membrane potential (MMP) in these two kinds of cells. N- acetyl-L cysteine (NAC), as a radical scavenger, significantly inhibited the generation of ROS and decreased the apoptosis of MCF-7 and BT-20 cells. Moreover, the expression of mitochondrial apoptosis-related proteins was detected by western blot assay. SSCC up-regulated the expression of Bax, down-regulated the expression of Bcl-2, subsequently increased the release of cytochrome c from mitochondria to cytoplasm, and activated the cleavage of caspase-9 and -3, which finally induced apoptosis in MCF-7 and BT-20 cells in vitro. Consequently, these data indicated that SSCC could induce apoptosis of MCF-7and BT-20 cells in vitro by mitochondrial pathway.

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Section: Introductionmentioning

confidence: 99%

Seleno-short-chain chitosan induces apoptosis in human breast cancer cells through mitochondrial apoptosis pathway in vitro

Zhao

et al. 2018

Cell Cycle

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“…The results were analyzed for each cell line tested using a GI% scale as follows: samples with low cytotoxic activity have GI < 50%; samples with moderate cytotoxic activity have GI between 50% and 75%, and samples with high cytotoxic activity have GI > 75% (25). The results showed that the extracts of P. cincinnata (3), P. gibertii (4), P. maliforms (5), P. mallacophyla (6), P. murchronata (7), P. morifolia (8) P. racemosa (10), P. setacea (11), P. suberosa (12), P. tenuifila (13) and P. vitifolia (14) have low or nonexistent cytotoxic activity in all human cancer cell lines tested. Extracts from P. capsularis (2) and P. quadrangulares (9) have high activity against HCT-116, low activity against SF-295, and cytotoxic activity from low ( P. quadrangulares ) to moderate ( P. capsularis ) against OVACAR-8.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the cytotoxic potential of extracts from the genus Passiflora cultived in Brazil against cancer cells

Svf

Âr

et al. 2018

Preprint

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Abstract20 This work aimed to evaluate the cytotoxic potential against cancer cells of Passiflora genus 21 plant species cultivated in Brazil and identify the mechanism of cytotoxicity induced by the 22 most promising extract. Leaf extracts from 14 Passiflora (P.) species were obtained ASE and 23 in vitro cytotoxicity evaluated against cancer cell lines using MTT assay at a single . CC-BY 4.0 International license It is made available under a (which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.

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“…Acridine orange (AO) is vital dyes that will stain both live and dead cells, and makes the nuclei appear green. Whereas, ethidium bromide (EtBr) is only taken up by those cells that have lost their cytoplasmic membrane integrity, and stains the nucleus as red [22] . Our results showed, Lupeol analogue (oxime) treated groups induced apoptosis, which is evidenced by the formation of nuclear changes precede cytoplasmic blebbing and nuclear fragmentation.…”

Section: Discussionmentioning

confidence: 99%

Chemotherapeutic potiential of Lupeol and its analogue (oxime) on human mammary cancer cell line (MCF-7)

Gunasekaran

Mirunalini

2017

Prog Biosci Bioeng

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Lupeol, a dietary triterpene, present in fruits and medicinal plants, has been reported to possess pharmacological properties including anticancer activities both in vitro and in vivo. The present study investigates the anti-cancer activity and the underlying mechanisms human mammary cancer (MCF-7). We observed increased levels of antioxidants (ABTS, DPPH, superoxide anions radical scavenging activity and hydroxyl radical scavenging activity) in MCF-7 cell line. The higher concentrations of lupeol analogues significantly increased cytotoxicity in MCF-7 cells. The IC 50 value was found to be 40 µM/mL of lupeol analogue (oxime) could greatly inhibit the cell growth. So, we have chosen at 40 and 320 µM/mL concentration of lupeol analogue (oxime) for further experiments. We observed increased levels of Lipid peroxidation and decreased levels of Antioxidants (SOD, CAT, GPx and GSH) in MCF-7 cell line because of the pro oxidant mechanism of lupeol analogue (oxime). These observations revealed the cytotoxic potential of lupeol, which could be attributed to their pro oxidant property on the MCF-7 cells. It is evident from the observation made in the present study that the lupeol analogue (oxime) has potential anticancer effect compared to Lupeol.

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Chimaphilin induces apoptosis in human breast cancer MCF-7 cells through a ROS-mediated mitochondrial pathway

Cited by 54 publications

References 36 publications

Seleno-short-chain chitosan induces apoptosis in human breast cancer cells through mitochondrial apoptosis pathway in vitro

Seleno-short-chain chitosan induces apoptosis in human breast cancer cells through mitochondrial apoptosis pathway in vitro

Evaluation of the cytotoxic potential of extracts from the genus Passiflora cultived in Brazil against cancer cells

Chemotherapeutic potiential of Lupeol and its analogue (oxime) on human mammary cancer cell line (MCF-7)

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