Assessment of endothelial function by acetylcholine iontophoresis: Impact of inter-electrode distance and electrical cutaneous resistance

Puissant, Cyril; Ábrahám, Pierre; Durand, Sylvain; Humeau-Heurtier, Anne; Faure, Sébastien; Lefthériotis, Georges

doi:10.1016/j.mvr.2014.04.001

Cited by 8 publications

(9 citation statements)

References 38 publications

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“…For example, post‐occlusive reactive hyperaemia stimulates both the endothelial and the non‐endothelial pathway . Sodium nitroprusside and acetylcholine iontophoresis investigate the non‐endothelial and the endothelial pathways, respectively . Gohin et al proposed to use CIV to investigate the vascular function of prostacyclin in rats .…”

Section: Discussionmentioning

confidence: 99%

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Evidence for a vasomotor cyclo‐oxygenase dependent mechanism of sensitization at the cutaneous level

Ábrahám

Humeau-Heurtier

Gascoin

et al. 2015

Brit J Clinical Pharma

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AIMSCurrent-induced vasodilation (CIV) is an axon-reflex response observed during monopolar current application such as iontophoresis. Cyclo-oxygenase derivates (COD) participate in CIV and act as sensitizing agents at the anodal level. Mechanisms involved during cathodal current application (CCA) are partially unknown. In a randomized double-blind crossover trial, we tested in 16 healthy subjects (i) the influence of the inter-stimulation interval (I-I) by comparing CIV following all-at-once 10 s CCA against 2 × 5 s CCA with intervals ranging from15 s-16 min and (ii) the participation of COD in CIV using 1 g aspirin or placebo intake. METHODSMeasurements were repeated 2 h and 14 days after treatment. Laser Doppler flowmetry assessed cutaneous blood flow, reported in multiples of baseline. RESULTSBefore treatment, peak vasodilation 10 min after the last current application (CVC stim2 ) increased compared with baseline whatever the I-I. Increase in CVC stim2 from baseline was greater for the 4 min (9.4 (5.3, 10.9) times; median (1 st percentile, 3 rd percentile)) and higher I-Is compared with all-at-once delivery (3.0 (2.1, 4.3) times, P < 0.05). The response was similar after placebo but aspirin abolished this vasodilation (increase by 1.2 (1.1, 1.3) times for all-at-once delivery and by 1.5 (1.3, 1.7) ± 0.3 times for 4 min interval, 2 h after aspirin intake) that recovered after 14 days. CONCLUSIONSThis confirms the participation of COD in CIV with CCA and their sensitizing action. This model can represent an attractive way to study the axon-reflex and sensitizing function of COD in humans. WHAT IS ALREADY KNOWN ABOUT THIS SUBJECT• Monopolar current applications (segmented or not) at the cutaneous level can elicit vasodilatation.• Cyclo-oxygenase derivates (probably prostaglandins) participate in this phenomenon and, during segmented current applications, can have a sensitizing effect on the nervous afferent stimulated. • This effect is well known at the anodal level but must be better described for cathodal current. WHAT THIS STUDY ADDS• Participation of cyclo-oxygenase derivates was confirmed for cathodal segmented current application.• A minimal interval must be respected between two current applications to observe the sensitizing effect of cyclooxygenase derivates.• This model is useful to study the sensitizing function of cyclo-oxygenase derivates in humans.

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Section: Discussionmentioning

confidence: 99%

“…The ‘active’ electrodes were also connected to current suppliers (Periiont micropharmacology System, PF 382 Perimed, Jarfalla, Sweden). For those electrodes, a disposable adhesive electrode (Kendall) was positioned 5 cm away from the laser probes, forming the anode and closing the current system (Figure ).…”

Section: Methodsmentioning

confidence: 99%

Evidence for a vasomotor cyclo‐oxygenase dependent mechanism of sensitization at the cutaneous level

Ábrahám

Humeau-Heurtier

Gascoin

et al. 2015

Brit J Clinical Pharma

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“…Iontophoresis of acetylcholine (ACh) and sodium nitroprusside (SNP) is a tool used to determine microvascular endothelial function. Two laser-Doppler probes specifically designed with an embedded sponge for skin iontophoresis of agonists are fixed 5 cm apart to the skin with an adhesive patch to measure the endothelium-dependent and endothelium-independent vasodilatation to determine the endothelial function 27) . The nailfold capillary video microscopy of the dorsal skin of the third finger is used to determine the endothelial function.…”

Section: Methodsmentioning

confidence: 99%

Association of Subjective and Objective Sleep Duration as well as Sleep Quality with Non-Invasive Markers of Sub-Clinical Cardiovascular Disease (CVD): A Systematic Review

Aziz

Ali

Das

et al. 2017

J Atheroscler Thromb

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Aim: Abnormal daily sleep duration and quality have been linked to hypertension, diabetes, stroke, and overall cardiovascular disease (CVD) morbidity & mortality. However, the relationship between daily sleep duration and quality with subclinical measures of CVD remain less well studied. This systematic review evaluated how daily sleep duration and quality affect burden of subclinical CVD in subjects free of symptomatic CVD.Methods: Literature search was done via MEDLINE, EMBASE, Web of Science until June 2016 and 32 studies met the inclusion criteria. Sleep duration and quality were measured either via subjective methods, as self-reported questionnaires or Pittsburg Sleep Quality Index (PSQI) or via objective methods, as actigraphy or polysomnography or by both. Among subclinical CVD measures, coronary artery calcium (CAC) was measured by electron beam computed tomography, Carotid intima-media thickness (CIMT) measured by high-resolution B-mode ultrasound on carotid arteries, endothelial/microvascular function measured by flow mediated dilation (FMD) or peripheral arterial tone (PAT) or iontophoresis or nailfold capillaroscopy, and arterial stiffness measured by pulse wave velocity (PWV) or ankle brachial index (ABI).Results: Subjective short sleep duration was associated with CAC and CIMT, but variably associated with endothelial dysfunction (ED) and arterial stiffness; however, subjective long sleep duration was associated with CAC, CIMT and arterial stiffness, but variably associated with ED. Objective short sleep duration was positively associated with CIMT and variably with CAC but not associated with ED. Objective long sleep duration was variably associated with CAC and CIMT but not associated with ED. Poor subjective sleep quality was significantly associated with ED and arterial stiffness but variably associated with CAC and CIMT. Poor objective sleep quality was significantly associated with CIMT, and ED but variably associated with CAC.Conclusions: Overall, our review provided mixed results, which is generally in line with published literature, with most of the studies showing a significant relationship with subclinical CVD, but only some studies failed to demonstrate such an association. Although such mechanistic relationship needs further evaluation in order to determine appropriate screening strategies in vulnerable populations, this review strongly suggested the existence of a relationship between abnormal sleep duration and quality with increased subclinical CVD burden.

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“…Each iontophoresis test requires one drug delivery electrode (LI 611 Drug Delivery Electrode (Perimed AB, Stockholm, Sweden)) together with one counter‐electrode (PF 384 Dispersive Electrode (Perimed AB, Stockholm, Sweden)). Acetylcholine (Miochol‐E, Bausch & Lomb Nordic AB, Bagsværd, Danmark) was used in the drug delivery electrode to study the microcirculatory response 38 . The setup was similar to the PORH setup but with the acetylcholine iontophoresis electrodes coupled on the forearm during LSCI‐measurement and whiteout occlusion from a blood pressure cuff.…”

Section: Methodsmentioning

confidence: 99%

Successful improved peripheral tissue perfusion was seen in patients with atherosclerosis after 12 months of treatment with aged garlic extract

Lindstedt

Wlosinska

Nilsson

et al. 2021

International Wound Journal

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Patients with arteriolosclerosis have impaired microvascular perfusion leading to impaired wound healing. Aged garlic extract has shown to have a positive impact on vascular elasticity. The present study aimed to assess the effect of long‐term treatment with AGE on peripheral tissue perfusion in patients with confirmed atherosclerosis. Ninety three patients with a CT‐scan confirmed coronary artery arteriolosclerosis were randomised in a double‐blind manner to placebo or 2400 mg AGE daily for 1 year. Peripheral tissue perfusion was evaluated at 0‐ and 12‐months using Laser Speckle Contrast Imaging. Measurement of post occlusive reactive hyperemia (PORH) and cutaneous vascular conductance (CVC) using acetylcholine iontophoresis (Ach) was conducted. After 12 months a significant increase of 21.6% (95% CI 3.2%‐40.0%, P < .05) was seen in the relative change of PORH in the AGE compared with the placebo group. The same response was seen for CVC and Ach with an increase of 21.4% (95% CI 3.4%‐39.4%, P < .05) in the AGE group compared with the placebo group. Aged garlic extract regenerated peripheral tissue perfusion and increase microcirculation in patients with arteriolosclerosis. Adequate peripheral tissue perfusion and tissue oxygen tension are important prerequisites for successful tissue repair. Restored microcirculation in patients could hypothetically facilitate wound healing.

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Assessment of endothelial function by acetylcholine iontophoresis: Impact of inter-electrode distance and electrical cutaneous resistance

Cited by 8 publications

References 38 publications

Evidence for a vasomotor cyclo‐oxygenase dependent mechanism of sensitization at the cutaneous level

Evidence for a vasomotor cyclo‐oxygenase dependent mechanism of sensitization at the cutaneous level

Association of Subjective and Objective Sleep Duration as well as Sleep Quality with Non-Invasive Markers of Sub-Clinical Cardiovascular Disease (CVD): A Systematic Review

Successful improved peripheral tissue perfusion was seen in patients with atherosclerosis after 12 months of treatment with aged garlic extract

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