Protective effect of flavonoid extract from Chinese bayberry (Myrica rubra Sieb. et Zucc.) fruit on alcoholic liver oxidative injury in mice

Abstract

To evaluate the beneficial effects of Chinese bayberry (Myrica rubra Sieb. et Zucc.) flavonoid extract (CBFE) on chronic alcohol-induced liver oxidative injury in mice, experimental mice were pretreated with different doses of CBFE (50-200 mg/kg) for 4 weeks by gavage feeding. Biochemical markers and enzymatic antioxidants from serum, liver tissue, mitochondria and microsomes were examined. Our results showed that the activities of TC, TG, L-DLC in serum, the activity of CYP2E1 in microsomes, and the levels of MDA in liver tissue and mitochondria, decreased significantly (P < 0.05) in the CBFE-treated group compared with the alcohol group. On the contrary, the activities of ALT, AST, and H-DLC in serum, enzymatic antioxidants GSH-Px, SOD and GST in liver tissue and mitochondria, and HO-1 in microsomes rose markedly (P < 0.05). Histopathological examination revealed that CBFE (200 mg/kg) pretreatment noticeably prevented alcohol-induced hepatocyte apoptosis and fatty degeneration. It was suggested that the hepatoprotective effects exhibited by CBFE on alcohol-induced liver oxidative injury may be due to its potent antioxidant properties.

Natural compounds in the chemoprevention of alcoholic liver disease

“…Data indicate that levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (L‐DLC), and malondialdehyde (MDA), along with activities of alanine aminotransferase (ALT), aspartate transaminase (AST), and CYP2E1, decreased remarkably in the flavonoid extract‐treated mice. Moreover, the activities of enzymatic antioxidant glutathione peroxidase (GSH‐Px), superoxide dismutase (SOD), glutathione S‐transferase (GST) in liver tissue and mitochondria and heme oxygenase‐1 (HO‐1) in microsomes increased significantly by flavonoid extract (Liu, Qi, Cao, & Li, ). Flavone isoorientin from Gypsophila elegans also behaves with a protective effect on alcoholic induced hepatic fibrosis in rats.…”

“…also plays an essential role in ethanol oxidation. Although CYP2E1 accounts for 10% ethanol metabolism under physiological conditions, it is induced by its substrates, especially by ethanol (Lu & Cederbaum, 2008 Moreover, the activities of enzymatic antioxidant glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), glutathione Stransferase (GST) in liver tissue and mitochondria and heme oxygenase-1 (HO-1) in microsomes increased significantly by flavonoid extract (Liu, Qi, Cao, & Li, 2014). Flavone isoorientin from Gypsophila elegans also behaves with a protective effect on alcoholic induced hepatic fibrosis in rats.…”

Platycodin D isolated from the aerial parts of Platycodon grandiflorum protects alcohol-induced liver injury in mice

“…2 Among the mechanisms implicated in the pathogenesis of ALD, oxidative stress and inflammatory response are known to play a pivotal role in the pathogenesis of alcohol induced liver injury. 5,6 Therefore, identifying a predominant source of antioxidants and their role in preventing ethanol-induced liver injury is an important target. 5,6 Therefore, identifying a predominant source of antioxidants and their role in preventing ethanol-induced liver injury is an important target.…”

Natural Products for Prevention and Treatment of Chemical‐Induced Liver Injuries

“…fruit, Pterocarpus santalinus , and Lysimachia christinae as well as quercetin administrated to rodents with ALI, respectively, pathological improvement of liver was observed, due to the decreased oxidative stress and inflammation (Bulle, Reddy, Padmavathi, Maturu, & Ch, ; Noorani & Kale, ; Peng et al., ; Wang et al., ). Besides, the dose‐dependent anti‐apoptotic effects and modulation of fat metabolism were particularly reported in rodent models treated with the extracts of Tamarindus indica leaf, Elephantopus scaber , Hovenia dulcis , or Chinese bayberry (Ghoneim & Eldahshan, ; Ho and others ; Liu, Qi, Cao, & Li, ; Wang et al., ). In addition, Penthorum chinense extract was found to prevent ALI in mice by reducing oxidative stress and ameliorating dysfunctional white adipose tissue‐derived fatty acid influx to the liver (Cao et al., ).…”