A systematic proteomic analysis of NaCl-stressed germinating maize seeds

Meng, Lei; Chen, Yibo; Lu, Tian-Cong; Wang, Yuefeng; Qian, Chunrong; Yu, Yang; Ge, Xuan-Liang; Li, Xiaohui; Wang, Baichen

doi:10.1007/s11033-014-3205-7

Cited by 26 publications

(13 citation statements)

References 60 publications

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“…In the majority of studies, the changes at the proteomic level under stress conditions have been analyzed after several hours or even days of exposure to stress. Thus, what happens within the initial shock phase of stress is poorly understood (Meng et al, 2014; Gong et al, 2015). It is known that biotic and abiotic stress have negative effects on photosynthesis.…”

Section: Discussionmentioning

confidence: 99%

The Physcomitrella patens Chloroplast Proteome Changes in Response to Protoplastation

et al. 2016

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Plant protoplasts are widely used for genetic manipulation and functional studies in transient expression systems. However, little is known about the molecular pathways involved in a cell response to the combined stress factors resulted from protoplast generation. Plants often face more than one type of stress at a time, and how plants respond to combined stress factors is therefore of great interest. Here, we used protoplasts of the moss Physcomitrella patens as a model to study the effects of short-term stress on the chloroplast proteome. Using label-free comparative quantitative proteomic analysis (SWATH-MS), we quantified 479 chloroplast proteins, 219 of which showed a more than 1.4-fold change in abundance in protoplasts. We additionally quantified 1451 chloroplast proteins using emPAI. We observed degradation of a significant portion of the chloroplast proteome following the first hour of stress imposed by the protoplast isolation process. Electron-transport chain (ETC) components underwent the heaviest degradation, resulting in the decline of photosynthetic activity. We also compared the proteome changes to those in the transcriptional level of nuclear-encoded chloroplast genes. Globally, the levels of the quantified proteins and their corresponding mRNAs showed limited correlation. Genes involved in the biosynthesis of chlorophyll and components of the outer chloroplast membrane showed decreases in both transcript and protein abundance. However, proteins like dehydroascorbate reductase 1 and 2-cys peroxiredoxin B responsible for ROS detoxification increased in abundance. Further, genes such as thylakoid ascorbate peroxidase were induced at the transcriptional level but down-regulated at the proteomic level. Together, our results demonstrate that the initial chloroplast reaction to stress is due changes at the proteomic level.

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Section: Discussionmentioning

confidence: 99%

The Physcomitrella patens Chloroplast Proteome Changes in Response to Protoplastation

et al. 2016

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“…This effect may delay germination since high salinity levels induce to the toxic effects on seeds (Parihar et al, 2015). Toxicity leads to changes in enzymatic activity (Gomes-Filho et al, 2008), in protein metabolism (Meng et al, 2014), hormonal imbalance (Ozhan and Hajibabaei, 2013) and reduction in seed reserves use (Othman et al, 2006).…”

Section: Germination Percentage Of Cultivar Brs Ag Under Different Namentioning

confidence: 99%

Morphophysiological analysis and expression of proline genes in rice (Oryza sativa L. cv. BRS AG) subjected to in vitro salt stress

Rossatto¹,

Maia²,

Amaral³

et al. 2018

Aust J Crop Sci

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About 20% of the cultivated land and almost half of the irrigated lands of the planet are affected by the salinization of the soil and the water. Rice cultivar BRS AG is the first grown for other purposes than human consumption and can be used in the production of ethanol and animal feed. The objective of this study was to evaluate the germination, morphological characteristics, proline accumulation, and the expression of the genes involved in the metabolism of proline in rice plants, cv. BRS AG, cultured in vitro under salt stress. The plants were cultivated in a culture MS medium with six different NaCl concentrations (0, 32, 68, 102, 136, 170 mM). The germination was evaluated at days 7 and 14, and morphological characteristics were analyzed only at day 21. For proline quantification and expression of the genes required for its metabolism (P5CS1, P5CS2, P5CR, OAT, P5CDH and PDH), two concentrations (136 mM and 0 mM) of NaCl were used, and the samples were collected at days 5, 10, 15, and 20. The results indicate that the growth of rice cv. BRS is completely impaired at 170 mM NaCl, and at 136 mM NaCl. Regarding proline content, in stressed plants, it was higher (1.24 mM g-1 MF) than in the control plants, and was directly related to a high expression observed for the P5CS2 (QR=5.50) and OAT (QR=5.44) genes at 20 days of stress, which explains the high proline concentration at this period. According to the results, rice cultivar BRS AG, is considered tolerant to salt stress.

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“…At 15 and 30 min after the onset of stresses (e.g., UV-B light, drought, and cold), the initial transcriptional changes in Arabidopsis are significant (Kilian et al, 2007 ). Currently, crop proteomic changes are often analyzed after several hours, even days after a stress onset (e.g., Meng et al, 2014 ). Thus, the initial proteome changes of crops under abiotic conditions should be further analyzed.…”

Section: Dissecting Cell or Tissue Specific Stress Responsementioning

confidence: 99%

Proteomic analysis of crop plants under abiotic stress conditions: where to focus our research?

Gong

Wang

2015

Front. Plant Sci.

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A systematic proteomic analysis of NaCl-stressed germinating maize seeds

Cited by 26 publications

References 60 publications

The Physcomitrella patens Chloroplast Proteome Changes in Response to Protoplastation

The Physcomitrella patens Chloroplast Proteome Changes in Response to Protoplastation

Morphophysiological analysis and expression of proline genes in rice (Oryza sativa L. cv. BRS AG) subjected to in vitro salt stress

Proteomic analysis of crop plants under abiotic stress conditions: where to focus our research?

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