Proteomic analysis of crop plants under abiotic stress conditions: where to focus our research?

Gong, Fangping; Hu, Xiuli; Wang, Wei

doi:10.3389/fpls.2015.00418

Cited by 40 publications

(21 citation statements)

References 44 publications

(47 reference statements)

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“…However, to date there was no in-depth study of the moss cell proteome at the initial stage of complex, multifactorial stress. Also, little is known about initial stress response in crops (Gong et al, 2015). Using a SWATH-MS approach, we performed label-free comparative quantitative proteomic analysis of chloroplasts isolated from protonemata and protoplasts of the moss P. patens .…”

Section: Discussionmentioning

confidence: 99%

“…In the majority of studies, the changes at the proteomic level under stress conditions have been analyzed after several hours or even days of exposure to stress. Thus, what happens within the initial shock phase of stress is poorly understood (Meng et al, 2014; Gong et al, 2015). It is known that biotic and abiotic stress have negative effects on photosynthesis.…”

Section: Discussionmentioning

confidence: 99%

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The Physcomitrella patens Chloroplast Proteome Changes in Response to Protoplastation

et al. 2016

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Plant protoplasts are widely used for genetic manipulation and functional studies in transient expression systems. However, little is known about the molecular pathways involved in a cell response to the combined stress factors resulted from protoplast generation. Plants often face more than one type of stress at a time, and how plants respond to combined stress factors is therefore of great interest. Here, we used protoplasts of the moss Physcomitrella patens as a model to study the effects of short-term stress on the chloroplast proteome. Using label-free comparative quantitative proteomic analysis (SWATH-MS), we quantified 479 chloroplast proteins, 219 of which showed a more than 1.4-fold change in abundance in protoplasts. We additionally quantified 1451 chloroplast proteins using emPAI. We observed degradation of a significant portion of the chloroplast proteome following the first hour of stress imposed by the protoplast isolation process. Electron-transport chain (ETC) components underwent the heaviest degradation, resulting in the decline of photosynthetic activity. We also compared the proteome changes to those in the transcriptional level of nuclear-encoded chloroplast genes. Globally, the levels of the quantified proteins and their corresponding mRNAs showed limited correlation. Genes involved in the biosynthesis of chlorophyll and components of the outer chloroplast membrane showed decreases in both transcript and protein abundance. However, proteins like dehydroascorbate reductase 1 and 2-cys peroxiredoxin B responsible for ROS detoxification increased in abundance. Further, genes such as thylakoid ascorbate peroxidase were induced at the transcriptional level but down-regulated at the proteomic level. Together, our results demonstrate that the initial chloroplast reaction to stress is due changes at the proteomic level.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Physcomitrella patens Chloroplast Proteome Changes in Response to Protoplastation

et al. 2016

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“…In addition to changes in protein abundance under stress, alterations in PTMs may occur, which can dramatically increase the complexity of the proteome without a concomitant increase in gene expression and affect properties such as protein activity, stability, and localization . In specific cases, PTMs play a more significant role than changes in protein abundance . Therefore, systematic research on PTMs at proteome level is a prerequisite for deeply understanding the mechanism behind crop stress response and tolerance.…”

Section: Introductionmentioning

confidence: 99%

Advances in crop proteomics: PTMs of proteins under abiotic stress

Gong

Cao

et al. 2016

Proteomics

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Under natural conditions, crop plants are frequently subjected to various abiotic environmental stresses such as drought and heat wave, which may become more prevalent in the coming decades. Plant acclimation and tolerance to an abiotic stress are always associated with significant changes in PTMs of specific proteins. PTMs are important for regulating protein function, subcellular localization and protein activity and stability. Studies of plant responses to abiotic stress at the PTMs level are essential to the process of plant phenotyping for crop improvement. The ability to identify and quantify PTMs on a large-scale will contribute to a detailed protein functional characterization that will improve our understanding of the processes of crop plant stress acclimation and stress tolerance acquisition. Hundreds of PTMs have been reported, but it is impossible to review all of the possible protein modifications. In this review, we briefly summarize several main types of PTMs regarding their characteristics and detection methods, review the advances in PTMs research of crop proteomics, and highlight the importance of specific PTMs in crop response to abiotic stress.

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“…The differentially expressed genes (DEGs) are transcripted and translated to proteins and, at this level, undergo other regulational, post-translational modifications (PTMs). PTMs regulate protein function, subcellular localization, correct folding, and stability (Gong et al 2015;Wu et al 2016). The studies on PTMs of stressed plants show that the protein phosphorylation and glycosylation are accelerated under stressful conditions (Hu et al 2013;Mustafa & Komatsu 2014).…”

Section: Overview Of Plant Salt-responsive Molecular Mechanismsmentioning

confidence: 99%

Molecular response of canola to salt stress: insights on tolerance mechanisms

Shokri-Gharelo

Motie-Noparvar

2018

Preprint

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Canola (Brassica napus L.) is widely cultivated around the world for the production of edible oils and biodiesel fuel. Despite many canola varieties being described as 'salt-tolerant', plant yield and growth decline drastically with increasing salinity. Although many studies have resulted in better understanding of the many important salt-response mechanisms that control salt signaling in plants, detoxification of ions, and synthesis of protective metabolites, the engineering of salt-tolerant crops has only progressed slowly. Genetic engineering has been considered as an efficient method for improving the salt tolerance of canola but there are many unknown or little-known aspects regarding canola response to salinity stress at the cellular and molecular level. In order to develop highly salt-tolerant canola, it is essential to improve knowledge of the salt-tolerance mechanisms, especially the key components of the plant salt-response network. In this review, we focus on studies of the molecular response of canola to salinity to unravel the different pieces of the salt response puzzle. The paper includes a comprehensive review of the latest studies, particularly of proteomic and transcriptomic analysis, including the most recently identified canola tolerance components under salt stress, and suggests where researchers should focus future studies.

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Proteomic analysis of crop plants under abiotic stress conditions: where to focus our research?

Cited by 40 publications

References 44 publications

The Physcomitrella patens Chloroplast Proteome Changes in Response to Protoplastation

The Physcomitrella patens Chloroplast Proteome Changes in Response to Protoplastation

Advances in crop proteomics: PTMs of proteins under abiotic stress

Molecular response of canola to salt stress: insights on tolerance mechanisms

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