Comparison of Markers and Functional Attributes of Human Adipose-Derived Stem Cells and Dedifferentiated Adipocyte Cells from Subcutaneous Fat of an Obese Diabetic Donor

Watson, James; Patel, Niketa A.; Moor, Andrea N.; Patel, Rekha; Ghansah, Tomar; Mathur, Abhishek; Murr, Michel M.; Bickford, Paula C.; Gould, Lisa; Cooper, Denise R.

doi:10.1089/wound.2013.0452

Cited by 36 publications

(36 citation statements)

References 30 publications

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“…By using gel electrophoresis it could be demonstrated that HA secreted by orbital fibroblasts is primarily comprised of large polymers (>500,000 Dalton) [29,30]. Interleukin (IL)-1b-stimulated secretion and activation of the thyroidea stimulating hormone (TSH)-receptor by autoantibodies markedly increase HA production of ADMSCs and adipocytes.…”

Section: Subcutaneous Adipose Tissuementioning

confidence: 99%

Midfacial rejuvenation by hyaluronic acid fillers and subcutaneous adipose tissue – A new concept

Wollina

2015

Medical Hypotheses

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Section: Subcutaneous Adipose Tissuementioning

confidence: 99%

Midfacial rejuvenation by hyaluronic acid fillers and subcutaneous adipose tissue – A new concept

Wollina

2015

Medical Hypotheses

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“…Their high proliferative capacity has also been reported, which is a valuable aspect of cell culture for stem cell applications 2 . Studies with human DFAT cells indicated that they may be more efficient than ASC from the same donor, based on their replicative and differentiation capacity 18 . A recent case study supports that DFAT cells were more efficient to differentiate into adipocytes and osteoblasts, and had higher telomerase levels than ASC from the same individual, a donor with obesity and diabetes 18 .…”

Section: Discussionmentioning

confidence: 99%

“…Studies with human DFAT cells indicated that they may be more efficient than ASC from the same donor, based on their replicative and differentiation capacity 18 . A recent case study supports that DFAT cells were more efficient to differentiate into adipocytes and osteoblasts, and had higher telomerase levels than ASC from the same individual, a donor with obesity and diabetes 18 . Thus, the use of ceiling culture may provide more efficient adipose stem cells than the already used ASC.…”

Section: Discussionmentioning

confidence: 99%

Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures

Lessard

Côté

Lapointe

et al. 2015

JoVE

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Mature adipocytes have been shown to reverse their phenotype into fibroblast-like cells in vitro through a technique called ceiling culture. Mature adipocytes can also be isolated from fresh adipose tissue for depot-specific characterization of their function and metabolic properties. Here, we describe a well-established protocol to isolate mature adipocytes from adipose tissues using collagenase digestion, and subsequent steps to perform ceiling cultures. Briefly, adipose tissues are incubated in a Krebs-Ringer-Henseleit buffer containing collagenase to disrupt tissue matrix. Floating mature adipocytes are collected on the top surface of the buffer. Mature cells are plated in a T25-flask completely filled with media and incubated upside down for a week. An alternative 6-well plate culture approach allows the characterization of adipocytes undergoing dedifferentiation. Adipocyte morphology drastically changes over time of culture. Immunofluorescence can be easily performed on slides cultivated in 6-well plates as demonstrated by FABP4 immunofluorescence staining. FABP4 protein is present in mature adipocytes but downregulated through dedifferentiation of fat cells. Mature adipocyte dedifferentiation may represent a new avenue for cell therapy and tissue engineering. Video LinkThe video component of this article can be found at

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“…Human ASCs are relatively heterogeneous and carry hematopoietic-associated markers such as CD11a, CD14, CD45, CD86 and HLA-DR, and low levels of the MSCs-associated markers CD13, CD29, CD34, CD44, CD63, CD73, CD90 and CD166 [73,74] . On the other hand, the DFAT cells are positive for CD13, CD29, CD44, CD90, CD105, CD9, CD166 and CD54, and negative for CD14, CD31, CD34, CD45, CD66b, CD106, CD117, CD133, CD146, CD271, CD309, HLA-DR and alpha-smooth muscle cell actin [1,50,75] . Both ASCS and DFAT cells express HLA-A, -B and -C, which suggests that both cell types have allogenic transplantation potential.…”

Section: Characterization Of Dfat Cellsmentioning

confidence: 99%

“…DFAT cells have emerged as a potential cell source for regenerative medicine because of their transdifferentiation capability and similarity to ASCs and bone marrow MSCs [75][76][77] . Multiple studies have demonstrated differentiation of DFAT cells into multiple lineages including adipogenic, osteogenic, chondrogenic, myogenic, angiogenic and neurogenic lineages (Figure 3).…”

Section: Differentiation Potential Of Dfat Cellsmentioning

confidence: 99%

Dedifferentiated fat cells: A cell source for regenerative medicine

Jumabay¹,

Boström²

2015

WJSC

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The identification of an ideal cell source for tissue regeneration remains a challenge in the stem cell field. The ability of progeny cells to differentiate into other cell types is important for the processes of tissue reconstruction and tissue engineering and has clinical, biochemical or molecular implications. The adaptation of stem cells from adipose tissue for use in regenerative medicine has created a new role for adipocytes. Mature adipocytes can easily be isolated from adipose cell suspensions and allowed to dedifferentiate into lipidfree multipotent cells, referred to as dedifferentiated fat (DFAT) cells. Compared to other adult stem cells, the DFAT cells have unique advantages in their abundance, ease of isolation and homogeneity. Under proper condition in vitro and in vivo , the DFAT cells have exhibited adipogenic, osteogenic, chondrogenic, cardiomyogenc, angiogenic, myogenic, and neurogenic potentials. In this review, we first discuss the phenomena of dedifferentiation and transdifferentiation of cells, and then dedifferentiation of adipocytes in particular. Understanding the dedifferentiation process itself may contribute to our knowledge of normal growth processes, as well as mechanisms of disease. Second, we highlight new developments in DFAT cell culture and summarize the current understanding of DFAT cell properties. The unique features of DFAT cells are promising for clinical applications such as tissue regeneration.

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Comparison of Markers and Functional Attributes of Human Adipose-Derived Stem Cells and Dedifferentiated Adipocyte Cells from Subcutaneous Fat of an Obese Diabetic Donor

Cited by 36 publications

References 30 publications

Midfacial rejuvenation by hyaluronic acid fillers and subcutaneous adipose tissue – A new concept

Midfacial rejuvenation by hyaluronic acid fillers and subcutaneous adipose tissue – A new concept

Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures

Dedifferentiated fat cells: A cell source for regenerative medicine

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