Characterization of polymorphisms in the mitochondrial DNA of twelve ethnic groups in the Guizhou province of China

Abstract

To characterize the genetic profiles and relationships between ancient ethnic populations, we analyzed polymorphisms in mitochondrial DNA (mtDNA) isolated from the blood of 753 members of 12 ethnic groups (Buyi, Dong, Gelao, Hui, Man, Miao, Menggu, Mulao, Maonan, Qiang, She and Zhuang) living in the Guizhou Province of China. The 9-bp deletion of mtDNA was detected by the polymerase chain reaction (PCR) and PCR-PAGE, and 11 SNPs by restriction fragment length polymorphism and mini-sequencing. Thereafter, these genotyping results were verified by PCR-DNA sequencing. The mtDNA of these populations exhibited considerable diversity, both with respect to the haplogroups M and N, and subgroups thereof. The differences between the major ethnic groups reflected the maternal inheritance. These ethnic groups in Guizhou demonstrated a genetic profile that differed considerably from that of other Asian populations. Our findings indicate that the matrilineal genetic profiles of Guizhou groups are relatively complex and distinct, showing relationships that reflect national history and geography.

Fine‐scale genetic structure of Tujia and central Han Chinese revealing massive genetic admixture under language borrowing

“…The genetic structure and relationship between Tujia and other related populations have mainly been investigated based on their forensics‐related genetic variations (Wei et al, 2006; Yuan et al, 2012; Shen et al, 2016; Sun et al, 2016) (STRs and InDels) or limited markers located on the mtDNA and Y chromosome (Shu et al, 2015; He et al, 2016; Liu et al, 2018; Feng et al, 2020). The population history of the Tibetan–Burman‐speaking Tujia people and the surrounding central Han Chinese has provoked intense debates over the past few decades.…”

Correlations between polymorphisms in the uridine diphosphate-glucuronosyltransferase 1A and C-C motif chemokine receptor 5 genes and infection with the hepatitis B virus in three ethnic groups in China

“…Genomic DNA was isolated from whole blood drawn from a vein in the elbow region (3 ml) using a kit based on extraction with phenol-chloroform and then redissolved in 10 mM tris-ethylenediaminetetra-acetic acid buffer (pH 8.0) as described previously (Shanghai Shenbo Chemical, Shanghai, China). 31 On the same day, the blood samples were extracted for DNA isolation at room temperature and the DNA was stored at –20°C. Before isolation, the blood samples were kept at 4°C.…”

Genomic formation of Tibeto-Burman speaking populations in Guizhou, Southwest China

“…Late Neolithic ancient DNA genomes from the Tibetan Plateau indicated the stable livelihood and genetic continuity in the past three thousand years on the Plateau [7][8][9][10][11][12]. The genetic legacy of Paleolithic huntergatherers was also found in the Y chromosome and mitochondrial DNA gene pool of the modern Tibeto-Burman populations on the Tibetan Plateau [13].…”