Biotin selective polymer nano-films

Abstract

BackgroundThe interaction between biotin and avidin is utilized in a wide range of assay and diagnostic systems. A robust material capable of binding biotin should offer scope in the development of reusable assay materials and biosensor recognition elements.ResultsBiotin-selective thin (3–5 nm) films have been fabricated on hexadecanethiol self assembled monolayer (SAM) coated Au/quartz resonators. The films were prepared based upon a molecular imprinting strategy where N,N'-methylenebisacrylamide and 2-acrylamido-2-methylpropanesulfonic acid were copolymerized and grafted to the SAM-coated surface in the presence of biotin methyl ester using photoinitiation with physisorbed benzophenone. The biotinyl moiety selectivity of the resonators efficiently differentiated biotinylated peptidic or carbohydrate structures from their native counterparts.ConclusionsMolecularly imprinted ultra thin films can be used for the selective recognition of biotinylated structures in a quartz crystal microbalance sensing platform. These films are stable for periods of at least a month. This strategy should prove of interest for use in other sensing and assay systems.

Study of the Interaction of Trastuzumab and SKOV3 Epithelial Cancer Cells Using a Quartz Crystal Microbalance Sensor

“…The first immunosensor based on piezoelectric detection was reported in 1972 by Shons and co-workers [ 18 , 19 , 20 ]. The QCM technique has since been undergone significant development, whereby today it is possible to use synthetic polymer-based antibody mimics [ 21 , 22 ] and even cells as sensor recognition elements [ 7 , 23 , 24 , 25 , 26 , 27 , 28 ].…”

A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format

“…There have been relatively few reports of the preparation of polymers imprinted with our chosen templates: fumonisin B2 MIPs for solid-phase extraction have been prepared by bulk polymerisation in acetonitrile8; glucosamine MIPs, prepared in aqueous buffer, have been reported for extraction of the monosaccharide from chicory root9; l -thyroxine-imprinted films have been prepared on electrodes for sensing applications1011; while biotin MIPs have been prepared as sol-gel magnetic core-shell particles12, as thin films1314 and in bulk format15. In contract to these diverse methods of synthesis, the imprinted polymer nanoparticles prepared in this work were all prepared in aqueous media, irrespective of the solubility or otherwise of the template molecules in water.…”

A two‐step strategy to visually identify molecularly imprinted polymers for tagged proteins

“…Biotin and its analogs are good templates that have previously been used to prepare MIPs mainly taking advantage of electrostatic interactions between the polymer and the carboxylic group besides the ureido functionality . Some successful attempts to achieve biotin binding MIPs have been described in the literature .…”