Study of the Interaction of Trastuzumab and SKOV3 Epithelial Cancer Cells Using a Quartz Crystal Microbalance Sensor

Elmlund, Louise; Käck, Camilla; Aastrup, Teodor; Nicholls, Ian A.

doi:10.3390/s150305884

Cited by 26 publications

(25 citation statements)

References 42 publications

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“…The antigen-binding fragment (Fab) mediates antigen recognition via complementaritydetermining regions and the crystallizable fragment (Fc) recruits Fc receptor on the immune cell or the other antibody recognition [86]. Trastuzumab, an antibody for FDA-approved antibody-drug conjugate Trastuzumab emtansine, has K D = 1-7 nM for the transmembrane tyrosine kinase receptor (HER2) [87][88][89]. Fab fragments can be used to reduce the bulky size of a full antibody (approximately 15 nm), alleviating immunogenicity and improving the pharmacokinetic profile of delivery vehicles [85,90].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Recent progress in development of siRNA delivery vehicles for cancer therapy

Kim

Miyata

et al. 2016

Advanced Drug Delivery Reviews

367

305

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Recent progress in RNA biology has broadened the scope of therapeutic targets of RNA drugs for cancer therapy. However, RNA drugs, typically small interfering RNAs (siRNAs), are rapidly degraded by RNases and filtrated in the kidney, thereby requiring a delivery vehicle for efficient transport to the target cells. To date, various delivery formulations have been developed from cationic lipids, polymers, and/or inorganic nanoparticles for systemic delivery of siRNA to solid tumors. This review describes the current status of clinical trials related to siRNA-based cancer therapy, as well as the remaining issues that need to be overcome to establish a successful therapy. It, then introduces various promising design strategies of delivery vehicles for stable and targeted siRNA delivery, including the prospects for future design.

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Section: Accepted Manuscriptmentioning

confidence: 99%

Recent progress in development of siRNA delivery vehicles for cancer therapy

Kim

Miyata

et al. 2016

Advanced Drug Delivery Reviews

367

305

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“…Introduction. In the modern world, biosensors have made a significant impact in many fields, such as antibody-antigen interactions, immunology, virology, and the pharmaceutical industry (Elmlund et al, 2015;Sanvicens et al, 2011). Hence, during the last few decades, there has been an accelerated technological development of biosensor instruments, e.g., surface plasmon resonance, quartz crystal microbalance, etc.…”

mentioning

confidence: 99%

Estimating the rate constant from biosensor data via an adaptive variational Bayesian approach

Zhang¹,

Yao²,

Forssén³

et al. 2019

Ann. Appl. Stat.

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The means to obtain the rate constants of a chemical reaction is a fundamental open problem in both science and the industry. Traditional techniques for finding rate constants require either chemical modifications of the reactants or indirect measurements. The rate constant map method is a modern technique to study binding equilibrium and kinetics in chemical reactions. Finding a rate constant map from biosensor data is an ill-posed inverse problem that is usually solved by regularization. In this work, rather than finding a deterministic regularized rate constant map that does not provide uncertainty quantification of the solution, we develop an adaptive variational Bayesian approach to estimate the distribution of the rate constant map, from which some intrinsic properties of a chemical reaction can be explored, including information about rate constants. Our new approach is more realistic than the existing approaches used for biosensors and allows us to estimate the dynamics of the interactions, which are usually hidden in a deterministic approximate solution. We verify the performance of the new proposed method by numerical simulations, and compare it with the Markov chain Monte Carlo algorithm. The results illustrate that the variational method can reliably capture the posterior distribution in a computationally efficient way. Finally, the developed method is also tested on the real biosensor data (parathyroid hormone), where we provide two novel analysis tools -the thresholding contour map and the high order moment map -to estimate the number of interactions as well as their rate constants.

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“…Quartz crystal microbalance (QCM) transducers are widely used mass‐sensitive sensors in the field of biosensor design, where the acoustic wave platforms serve as sensitive elements during surface binding events . The QCM has been also successfully applied to study cell adhesion, the surface spreading of living cells, the interaction of antibody with growth factor receptors of cancer cells, and cell responses when exposed to nanoparticles and nanotubes . Tymchenko et al used the QCM with dissipation monitoring (QCM‐D) biosensor setup to study the biomechanical properties of stem cell CM clusters.…”

Section: Methods Results and Discussionmentioning

confidence: 99%

Phenotypic assays for analyses of pluripotent stem cell–derived cardiomyocytes

Pešl

Přibyl

Caluori

et al. 2016

J of Molecular Recognition

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Stem cell-derived cardiomyocytes (CMs) hold great hopes for myocardium regeneration because of their ability to produce functional cardiac cells in large quantities. They also hold promise in dissecting the molecular principles involved in heart diseases and also in drug development, owing to their ability to model the diseases using patient-specific human pluripotent stem cell (hPSC)-derived CMs. The CM properties essential for the desired applications are frequently evaluated through morphologic and genotypic screenings. Even though these characterizations are necessary, they cannot in principle guarantee the CM functionality and their drug response. The CM functional characteristics can be quantified by phenotype assays, including electrophysiological, optical, and/or mechanical approaches implemented in the past decades, especially when used to investigate responses of the CMs to known stimuli (eg, adrenergic stimulation). Such methods can be used to indirectly determine the electrochemomechanics of the cardiac excitation-contraction coupling, which determines important functional properties of the hPSC-derived CMs, such as their differentiation efficacy, their maturation level, and their functionality. In this work, we aim to systematically review the techniques and methodologies implemented in the phenotype characterization of hPSC-derived CMs. Further, we introduce a novel approach combining atomic force microscopy, fluorescent microscopy, and external electrophysiology through microelectrode arrays. We demonstrate that this novel method can be used to gain unique information on the complex excitation-contraction coupling dynamics of the hPSC-derived CMs.

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Study of the Interaction of Trastuzumab and SKOV3 Epithelial Cancer Cells Using a Quartz Crystal Microbalance Sensor

Cited by 26 publications

References 42 publications

Recent progress in development of siRNA delivery vehicles for cancer therapy

Recent progress in development of siRNA delivery vehicles for cancer therapy

Estimating the rate constant from biosensor data via an adaptive variational Bayesian approach

Phenotypic assays for analyses of pluripotent stem cell–derived cardiomyocytes

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