Rolled-up Functionalized Nanomembranes as Three-Dimensional Cavities for Single Cell Studies

Xi, Wang; Schmidt, Christine K.; Sánchez, Samuel; Gracias, David H.; Carazo-Salas, Rafael E.; Jackson, Stephen; Schmidt, Oliver G.

doi:10.1021/nl4042565

Cited by 74 publications

(80 citation statements)

References 50 publications

(89 reference statements)

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“…[23] However, prior studies have focused predominantly on 2D patterns or the study of single cells in confined spaces, [11, 24, 25] and only recently have 3D patterned wells and surfaces started to be explored to investigate the collective behavior of cells. [26, 27] In our work, we utilized soft-lithographic techniques to address a fundamental question in cell biology: How does anisotropy alter the cell behavior of cell populations and their generated matrix in 3D anisotropic spaces?…”

Section: Discussionmentioning

confidence: 99%

Patterning of Fibroblast and Matrix Anisotropy within 3D Confinement is Driven by the Cytoskeleton

Serbo

Kuo

Lewis

et al. 2015

Adv Healthcare Materials

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Effects of 3D confinement on cellular growth and matrix assembly are important in tissue engineering, developmental biology, and regenerative medicine. Polydimethylsiloxane wells with varying anisotropy are microfabicated using soft‐lithography. Microcontact printing of bovine serum albumin is used to block cell adhesion to surfaces between wells. The orientations of fibroblast stress fibers, microtubules, and fibronectin fibrils are examined 1 day after cell seeding using laser scanning confocal microscopy, and anisotropy is quantified using a custom autocorrelation analysis. Actin, microtubules, and fibronectin exhibit higher anisotropy coefficients for cells grown in rectangular wells with aspect ratios of 1:4 and 1:8, as compared to those in wells with lower aspect ratios or in square wells. The effects of disabling individual cytoskeletal components on fibroblast responses to anisotropy are then tested by applying actin or microtubule polymerization inhibitors, Rho kinase inhibitor, or by siRNA‐mediated knockdown of AXL or cofilin‐1. Latrunculin A decreases cytoskeletal and matrix anisotropy, nocodazole ablates both, and Y27632 mutes cellular polarity while decreasing matrix anisotropy. AXL siRNA knockdown has little effect, as does siRNA knockdown of cofilin‐1. These data identify several specific cytoskeletal strategies as targets for the manipulation of anisotropy in 3D tissue constructs.

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Section: Discussionmentioning

confidence: 99%

Patterning of Fibroblast and Matrix Anisotropy within 3D Confinement is Driven by the Cytoskeleton

Serbo

Kuo

Lewis

et al. 2015

Adv Healthcare Materials

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“…For example, several groups have previously demonstrated a range of rolled-up and folded devices including energy storage, microfluidic, electromagnetic, and single cell devices that highlight these advantageous characteristics. 12,18,[26][27][28] Additionally, our FLG/SU-8 bilayers are optically transparent and thus relevant to metamaterial and imaging applications. Finally, it is important to note that the SU-8 self-folding mechanism is such that when the FLG/SU-8 bilayers are transferred from either acetone or water to air, they maintain their previous configuration over time.…”

Section: Indicates Simulation Parameters and (Figs 3(a)-3(d)) Shows mentioning

confidence: 99%

Self-folding graphene-polymer bilayers

Deng

Yoon

Jin

et al. 2015

Applied Physics Letters

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In order to incorporate the extraordinary intrinsic thermal, electrical, mechanical, and optical properties of graphene with three dimensional (3D) flexible substrates, we introduce a solvent-driven self-folding approach using graphene-polymer bilayers. A polymer (SU-8) film was spin coated atop chemically vapor deposited graphene films on wafer substrates and graphene-polymer bilayers were patterned with or without metal electrodes using photolithography, thin film deposition, and etching. After patterning, the bilayers were released from the substrates and they self-folded to form fully integrated, curved, and folded structures. In contrast to planar graphene sensors on rigid substrates, we assembled curved and folded sensors that are flexible and they feature smaller form factors due to their 3D geometry and large surface areas due to their multiple rolled architectures. We believe that this approach could be used to assemble a range of high performance 3D electronic and optical devices of relevance to sensing, diagnostics, wearables, and energy harvesting.

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“…[20, 30–32] These approaches require, however, integration of additional components and materials, and, therefore, significantly complicate the fabrication process. 3D structures formed via origami, [33, 34] buckling, [35–38] and 3D printing [39] have attracted significant attentions due to their wide range of applications such as microphysiological systems, [39] cell studies, [40, 41] bio-mimic actuators, [42, 43] and the control of wave propagation. [44, 45] However, their applications in MEMS resonators and energy harvesters remain to be fully explored.…”

Section: Introductionmentioning

confidence: 99%

3D Tunable, Multiscale, and Multistable Vibrational Micro‐Platforms Assembled by Compressive Buckling

Ning

Wang

et al. 2017

Adv Funct Materials

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Microelectromechanical systems remain an area of significant interest in fundamental and applied research due to their wide ranging applications. Most device designs, however, are largely two-dimensional and constrained to only a few simple geometries. Achieving tunable resonant frequencies or broad operational bandwidths requires complex components and/or fabrication processes. The work presented here reports unusual classes of three-dimensional (3D) micromechanical systems in the form of vibratory platforms assembled by controlled compressive buckling. Such 3D structures can be fabricated across a broad range of length scales and from various materials, including soft polymers, monocrystalline silicon, and their composites, resulting in a wide scope of achievable resonant frequencies and mechanical behaviors. Platforms designed with multistable mechanical responses and vibrationally de-coupled constituent elements offer improved bandwidth and frequency tunability. Furthermore, the resonant frequencies can be controlled through deformations of an underlying elastomeric substrate. Systematic experimental and computational studies include structures with diverse geometries, ranging from tables, cages, rings, ring-crosses, ring-disks, two-floor ribbons, flowers, umbrellas, triple-cantilever platforms, and asymmetric circular helices, to multilayer constructions. These ideas form the foundations for engineering designs that complement those supported by conventional, microelectromechanical systems, with capabilities that could be useful in systems for biosensing, energy harvesting and others.

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Rolled-up Functionalized Nanomembranes as Three-Dimensional Cavities for Single Cell Studies

Cited by 74 publications

References 50 publications

Patterning of Fibroblast and Matrix Anisotropy within 3D Confinement is Driven by the Cytoskeleton

Patterning of Fibroblast and Matrix Anisotropy within 3D Confinement is Driven by the Cytoskeleton

Self-folding graphene-polymer bilayers

3D Tunable, Multiscale, and Multistable Vibrational Micro‐Platforms Assembled by Compressive Buckling

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