Stress-induced endocrine response and anxiety: the effects of comfort food in rats

Ortolani, Daniela; Garcia, Márcia Teixeira; Melo-Thomas, Liana; Spadari-Bratfisch, Regina Célia

doi:10.3109/10253890.2014.898059

Cited by 32 publications

(23 citation statements)

References 52 publications

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“…To evaluate the experimental data, a two-way (restraint stress × gestational age) analysis of variance (ANOVA) was performed, followed by a Duncan posthoc test. The significant difference between control and stressed mice at a given gestational age was evaluated using Independent-samples t-test (Ortolani et al, 2014). In the data for food intake, n was the number of cages per group, while n was the number of mice per group for the other data collected.…”

Section: Discussionmentioning

confidence: 99%

“…Restraint stress during late gestation decreases body weight and food intake of the dams, and produces long-term metabolic changes (Mairesse et al, 2007). Moreover, restraint stress not only decreases food consumption (Ortolani et al, 2014), but also increases appetite for sweet food in rodents (Ely et al, 1997). Generally, maternal weight increases with increase in gestational age.…”

Section: Restraint Stress Decreased Body and Organ Weightmentioning

confidence: 98%

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Restraint stress delays endometrial adaptive remodeling during mouse embryo implantation

Liu¹,

Dong²,

Wang³

et al. 2015

Stress

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We showed previously in mice that restraint stress reduces the number of embryo implantation sites in the endometrium. Here we hypothesized that the uterine microenvironment is altered by restraint stress and consequently is sub-optimal for embryo implantation. On embryonic day 1 (E1), 60 of 154 pregnant CD1 mice underwent restraint stress (4h), repeated daily to E3, E5 or E7 (n = 10 mice per group). Restraint stress decreased food intake and suppressed body weight gain on E3, E5 and E7. Restraint stress decreased the actual and relative weight (percent body weight) of uterus and ovary on E5 (by 14.9%, P = 0.03; 16.1%, P = 0.004) and E7 (by 16.8%, P = 0.03; 20.0%, P = 0.01). Morphologically, restraint stress decreased relative endometrial area (by 8.94-18.8%, P = 0.003-0.021) and uterine gland area (by 30.6%, P < 0.01 on E3 and 44.5%, P < 0.01 on E5). Immunohistochemistry showed that restraint stress decreased microvessel density (by 12.9-70.5%, P < 0.01) and vascular endothelial growth factor expression (by 14.6-45.9%, P = 0.007-0.02). Restraint stress decreased by 32.4-39.8% (P = 0.002-0.01) the mean optical density ratio for proliferating cell nuclear antigen/terminal deoxynucleotidyl transferase dUTP nick end labeling. Methyl thiazolyl tetrazolium assay showed a dosedependent decrease in proliferative activity of endometrial stromal cells (from 52 of 154 pregnant E5 control mice) incubated with H 2 O 2 (100-1000 μM) in vitro. These findings supported the hypothesis that restraint stress negatively influences endometrial adaptive remodeling via an oxidative stress pathway, which resulted in fewer implantation sites. Stress Downloaded from informahealthcare.com by Nanyang Technological University on 08/26/15 For personal use only. Stress Downloaded from informahealthcare.com by Nanyang Technological University on 08/26/15 For personal use only. Stress Downloaded from informahealthcare.com by Nanyang Technological University on 08/26/15 For personal use only. Stress Downloaded from informahealthcare.com by Nanyang Technological University on 08/26/15 For personal use only. Stress Downloaded from informahealthcare.com by Nanyang Technological University on 08/26/15 For personal use only.

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Section: Discussionmentioning

confidence: 99%

Section: Restraint Stress Decreased Body and Organ Weightmentioning

confidence: 98%

Restraint stress delays endometrial adaptive remodeling during mouse embryo implantation

Liu¹,

Dong²,

Wang³

et al. 2015

Stress

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show abstract

“…The data were analyzed with SPSS 18.0 (SPSS Inc., Chicago, IL) as described in a previous study (Ortolani et al, 2014). To analyze the effects of restraint stress and different gestational stages on the experimental data, a two-way analysis of variance (ANOVA) was performed followed by Duncan's post hoc test.…”

Section: Discussionmentioning

confidence: 99%

Restraint stress alters immune parameters and induces oxidative stress in the mouse uterus during embryo implantation

Liu

Dong

Wang

et al. 2014

Stress

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The influence of stress on embryo implantation is not well understood. Prior studies have focused on later gestational stages and the long-term impact of stress on immune function. The objective of this study is to investigate the effects of restraint stress on the immune parameters and the oxidative states of the uterus during implantation. In this study, pregnant CD1 mice were subjected to restraint stress (4 h/d) on embryonic day 1 (E1) and sacrificed on E3, E5, and E7. Maternal plasma corticosterone (CORT) secretion and implantation sites in the uterus were examined. The uterine (excluding embryos) homogenate and uterine lymphocytes were collected to examine oxidative stress states and associated immune parameters. The results demonstrated that restraint stress increased maternal plasma CORT secretion and reduced the number of implantation sites by 15.3% on E5 and by 26.1% on E7. Moreover, restraint stress decreased the density of uterine natural killer (uNK) cells in the endometrium by 22.1-47.9% and increased the density of mast cells in the myometrium by 55.6-76.9%. Restraint stress remarkably decreased the CD3(+)CD4(+) T/CD3(+)CD8(+) T cell ratio (by 26.2-28.9%) and attenuated uterine lymphocyte proliferation and secretion of cytokines. In addition, restraint stress threatened the intracellular equilibrium between oxidants and antioxidants, resulting in decreased glutathione peroxidase (GSH-PX) (32.2% and 45.7%), superoxide dismutase (SOD) (15.5% and 26.1%), and total antioxidant capacity (T-AOC) (18.4% and 18.2%) activities and increased malondialdehyde (MDA) (34.4% and 43.0%) contents on E5 and E7. In conclusion, these findings demonstrate that restraint stress causes abnormal implantation and negatively impacts immune parameters in association with oxidative stress in mice.

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“…A number of chronic stress models including chronic mild, chronic variable, social instability, and social isolation have been used in female rats. Many of these studies report increased anxiety‐like behaviors in traditional behavioral assays including the elevated plus maze and open field (Haller et al, ; Dalla et al, ; Ortolani et al, ) and depression‐like behavior as indicated by decreased sucrose consumption and/or increased immobility in the forced swim test (Baker et al, ; Bourke and Neigh, ). In contrast, far fewer studies have investigated the impact of chronic stressors on subsequent social behavior in female rats.…”

Section: Introductionmentioning

confidence: 99%

Evaluating social defeat as a model for psychopathology in adult female rodents

Solomon

2016

J of Neuroscience Research

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Social conflict is a predominant stressor in humans and is associated with increased risk for developing psychological illnesses including depression and anxiety. Overwhelmingly, more women suffer from these disorders, which may be due to increased stress sensitivity. Like humans, rodents experience a myriad of physiological and behavioral sequelae due to prolonged stress exposure. Although the motivation for social conflict may differ between humans and rodents, female rodents may provide an opportunity to explore the underlying mechanisms by which stress confers risk for psychopathology in women. Because most female rodents do not express spontaneous aggression, the majority of basic research examines the physiological and behavioral outcomes of social conflict in male rodents. However, there are instances where female rodents exhibit territorial (California mice and Syrian hamsters) and maternal aggression (rats, mice, and hamsters) creating a venue to examine sex differences in physiology and behavior in response to stress. While many studies rely upon nonsocial behavioral assays (e.g., elevated plus maze, forced swim test) to assess the impact of stress on emotionality, here we primarily focus on behavioral outcomes in social-based assays in rodents. This is critically important given that disruptions in social relationships can be a cause and consequence of neuropsychiatric diseases. Next, we briefly discuss how sex differences in the recruitment of neural circuitry and/or neurochemistry in response to stress may underlie sex differences in neuroendocrine and behavioral stress responses. Finally, the translational value of females in rodent stress models and considerations regarding behavioral interpretations of these models are discussed. V C 2016 Wiley Periodicals, Inc.

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Stress-induced endocrine response and anxiety: the effects of comfort food in rats

Cited by 32 publications

References 52 publications

Restraint stress delays endometrial adaptive remodeling during mouse embryo implantation

Restraint stress delays endometrial adaptive remodeling during mouse embryo implantation

Restraint stress alters immune parameters and induces oxidative stress in the mouse uterus during embryo implantation

Evaluating social defeat as a model for psychopathology in adult female rodents

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